(Circular) (Six-base) MAPSORT of: pFN21AE0143.seq Check: 7164 from: 1 to: 5635 pFN21AE0143 5635 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2167 2956 2167 Size: 789 4846 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 3378 4950 3378 Size: 1572 4063 ApaI G_GGCC'C Cuts at: 2144 2158 2144 Size: 14 5621 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2305 2944 3170 3322 5431 1493 Size: 812 639 226 152 2109 1697 Fragments arranged by size: 2109 1697 812 639 226 152 AseI AT'TA_AT Cuts at: 160 1032 2796 4189 160 Size: 872 1764 1393 1606 Fragments arranged by size: 1764 1606 1393 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2960 1870 Size: 73 155 862 4545 Fragments arranged by size: 4545 862 155 73 BamHI G'GATC_C Cuts at: 2042 2965 3332 2042 Size: 923 367 4345 Fragments arranged by size: 4345 923 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2026 2060 2167 2244 2956 Size: 325 217 627 239 34 107 77 712 Cuts at: 2956 4411 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 712 627 325 239 217 107 77 34 BanII G_rGCy'C Cuts at: 1391 2144 2158 2625 2954 1391 Size: 753 14 467 329 4072 Fragments arranged by size: 4072 753 467 329 14 BbeI G_GCGC'C Cuts at: 2064 2064 Size: 5635 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 2882 928 Size: 971 326 657 3681 Fragments arranged by size: 3681 971 657 326 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 4246 Size: 107 122 71 631 605 337 9 2228 Cuts at: 4246 136 Size: 1525 Fragments arranged by size: 2228 1525 631 605 337 122 107 71 9 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 2144 2158 3696 4852 1790 Size: 354 14 1538 1156 2573 Fragments arranged by size: 2573 1538 1156 354 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3366 4324 442 Size: 416 53 83 399 24 1949 958 1753 Fragments arranged by size: 1949 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 4295 1239 Size: 1031 2025 2579 Fragments arranged by size: 2579 2025 1031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4298 882 Size: 617 2799 2219 Fragments arranged by size: 2799 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 3442 3824 4459 Size: 53 83 186 936 528 1381 382 635 Cuts at: 4459 275 Size: 1451 Fragments arranged by size: 1451 1381 936 635 528 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5410 Fragments arranged by size: 5410 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2270 1186 Size: 1081 3 4551 Fragments arranged by size: 4551 1081 3 BseYI C'CCAG_C Cuts at: 2163 4838 5334 2163 Size: 2675 496 2464 Fragments arranged by size: 2675 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5062 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2178 3085 3846 4874 1540 Size: 275 186 12 165 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 165 12 BsiHKAI G_wGCw'C Cuts at: 2625 3696 3781 4852 2625 Size: 1071 85 1071 3408 Fragments arranged by size: 3408 1071 1071 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 BsmBI CGTCTCn'nnnn_ Cuts at: 2703 2703 Size: 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2158 2625 2954 3696 3781 4852 Size: 399 354 14 467 329 742 85 1071 Cuts at: 4852 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 467 399 354 329 85 14 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 2990 844 Size: 1335 811 3489 Fragments arranged by size: 3489 1335 811 BsrBI CCG'CTC Cuts at: 1394 1823 2138 3522 1394 Size: 429 315 1384 3507 Fragments arranged by size: 3507 1384 429 315 BsrDI GCAATG_nn' Cuts at: 65 1248 2778 4130 4304 65 Size: 1183 1530 1352 174 1396 Fragments arranged by size: 1530 1396 1352 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2130 4279 4466 1700 Size: 110 320 2149 187 2869 Fragments arranged by size: 2869 2149 320 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstXI CCAn_nnnn'nTGG Cuts at: 2668 2668 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2042 2965 3332 3717 3734 5186 5272 5284 Size: 70 923 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 923 385 367 346 86 70 17 12 Bsu36I CC'TnA_GG Cuts at: 2757 2757 Size: 5635 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4135 Fragments arranged by size: 4135 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 2089 2673 3151 3945 3965 2089 Size: 584 478 794 20 3759 Fragments arranged by size: 3759 794 584 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2328 2363 2760 2795 529 Size: 35 1764 35 397 35 3369 Fragments arranged by size: 3369 1764 397 35 35 35 DraI TTT'AAA Cuts at: 2902 2939 2902 Size: 37 5598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2886 4642 809 Size: 507 1570 1756 1802 Fragments arranged by size: 1802 1756 1570 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2073 2175 Size: 54 1425 50 275 18 180 63 102 Cuts at: 2175 2613 2832 3082 3971 5600 8 Size: 438 219 250 889 1629 43 Fragments arranged by size: 1629 1425 889 438 275 250 219 180 102 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 2175 3082 1537 Size: 275 198 165 907 4090 Fragments arranged by size: 4090 907 275 198 165 EarI CTCTTCn'nnn_ Cuts at: 1353 3565 1353 Size: 2212 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2623 2623 Size: 5635 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 3698 4295 5082 5350 1239 Size: 393 638 1428 597 787 268 1524 Fragments arranged by size: 1524 1428 787 638 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2568 2615 2252 Size: 316 47 5272 Fragments arranged by size: 5272 316 47 EcoO109I rG'GnC_Cy Cuts at: 2034 2183 2762 2913 2034 Size: 149 579 151 4756 Fragments arranged by size: 4756 579 151 149 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2897 2909 2897 Size: 12 5623 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 2064 4782 1678 Size: 386 2718 2531 Fragments arranged by size: 2718 2531 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4276 4308 4350 4382 1373 Size: 32 135 32 2704 32 42 32 2626 Fragments arranged by size: 2704 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2979 3225 669 Size: 988 281 1041 246 3079 Fragments arranged by size: 3079 1041 988 281 246 HindIII A'AGCT_T Cuts at: 748 2844 748 Size: 2096 3539 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KasI G'GCGC_C Cuts at: 2060 2060 Size: 5635 KpnI G_GTAC'C Cuts at: 2171 2960 2171 Size: 789 4846 MfeI C'AATT_G Cuts at: 2442 3234 2442 Size: 792 4843 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2891 4749 4933 1305 Size: 1586 1858 184 2007 Fragments arranged by size: 2007 1858 1586 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 2834 10 Size: 54 1425 343 783 219 2811 Fragments arranged by size: 2811 1425 783 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 3593 3952 4111 518 Size: 648 1437 990 359 159 2042 Fragments arranged by size: 2042 1437 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 3051 3728 4876 5121 1606 Size: 409 1036 677 1148 245 2120 Fragments arranged by size: 2120 1148 1036 677 409 245 NaeI GCC'GGC Cuts at: 2132 2132 Size: 5635 NarI GG'CG_CC Cuts at: 2061 2061 Size: 5635 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NgoMIV G'CCGG_C Cuts at: 2130 2130 Size: 5635 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 2010 3082 2010 Size: 1072 4563 NspI r_CATG'y Cuts at: 1132 1229 2054 2993 4538 1132 Size: 97 825 939 1545 2229 Fragments arranged by size: 2229 1545 939 825 97 PasI CC'CwG_GG Cuts at: 1348 2158 2405 1348 Size: 810 247 4578 Fragments arranged by size: 4578 810 247 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2689 1108 Size: 1581 4054 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2183 2762 2183 Size: 579 5056 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 2327 3205 2327 Size: 878 4757 PspOMI G'GGCC_C Cuts at: 2140 2154 2140 Size: 14 5621 PstI C_TGCA'G Cuts at: 830 2987 830 Size: 2157 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 SacI G_AGCT'C Cuts at: 2625 2625 Size: 5635 SacII CC_GC'GG Cuts at: 2016 2016 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SanDI GG'GwC_CC Cuts at: 2183 2183 Size: 5635 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2203 2719 2983 4118 4799 4990 826 Size: 219 1158 516 264 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 681 516 264 219 191 SfoI GGC'GCC Cuts at: 2062 2062 Size: 5635 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2100 2962 1872 Size: 228 862 4545 Fragments arranged by size: 4545 862 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 3739 4640 4902 820 Size: 197 926 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2054 2993 1132 Size: 922 939 3774 Fragments arranged by size: 3774 939 922 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 2729 1517 Size: 1212 4423 StyI C'CwwG_G Cuts at: 513 1065 2004 2037 2616 2730 3074 513 Size: 552 939 33 579 114 344 3074 Fragments arranged by size: 3074 939 579 552 344 114 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3662 3679 3832 4017 4462 325 Size: 1255 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 2401 2433 5595 5627 2259 Size: 32 110 32 3162 32 2267 Fragments arranged by size: 3162 2267 110 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2098 2960 1870 Size: 228 862 4545 Fragments arranged by size: 4545 862 228 XmnI GAAnn'nnTTC Cuts at: 2889 3764 2889 Size: 875 4760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI SrfI SwaI XcmI