(Circular) (Six-base) MAPSORT of: pFN21AE0454.seq Check: 5223 from: 1 to: 5587 pFN21AE0454 5587 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 2391 Size: 5587 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 1937 Size: 993 4594 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3650 5034 5302 1632 Size: 2018 1384 268 1917 Fragments arranged by size: 2018 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2650 820 Size: 197 1633 3757 Fragments arranged by size: 3757 1633 197 AflIII A'CryG_T Cuts at: 1203 2616 4486 5463 1203 Size: 1413 1870 977 1327 Fragments arranged by size: 1870 1413 1327 977 AgeI A'CCGG_T Cuts at: 2127 4418 2127 Size: 2291 3296 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 3330 4902 3330 Size: 1572 4015 ApaI G_GGCC'C Cuts at: 2036 2036 Size: 5587 ApaLI G'TGCA_C Cuts at: 3644 4800 3644 Size: 1156 4431 ApoI r'AATT_y Cuts at: 1493 2532 2600 2663 2810 2896 3122 3274 5383 Size: 1039 68 63 147 86 226 152 2109 Cuts at: 5383 1493 Size: 1697 Fragments arranged by size: 2109 1697 1039 226 152 147 86 68 63 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2414 2912 1870 Size: 73 471 498 4545 Fragments arranged by size: 4545 498 471 73 BamHI G'GATC_C Cuts at: 2152 2917 3284 2152 Size: 765 367 4455 Fragments arranged by size: 4455 765 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2908 4363 618 Size: 325 217 627 1121 1455 1842 Fragments arranged by size: 1842 1455 1121 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2906 1391 Size: 645 870 4072 Fragments arranged by size: 4072 870 645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2808 2835 928 Size: 971 909 27 3680 Fragments arranged by size: 3680 971 909 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3778 3812 1903 Size: 34 50 34 1757 34 3678 Fragments arranged by size: 3678 1757 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3479 4689 3479 Size: 1210 4377 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 4198 136 Size: 107 122 71 631 605 368 2158 1525 Fragments arranged by size: 2158 1525 631 605 368 122 107 71 BglII A'GATC_T Cuts at: 5582 5582 Size: 5587 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 2036 3648 4804 1790 Size: 246 1612 1156 2573 Fragments arranged by size: 2573 1612 1156 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3318 4276 442 Size: 416 53 83 399 24 1901 958 1753 Fragments arranged by size: 1901 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 4247 1239 Size: 1088 1920 2579 Fragments arranged by size: 2579 1920 1088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3712 4577 4875 3712 Size: 865 298 4424 Fragments arranged by size: 4424 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4250 882 Size: 617 2751 2219 Fragments arranged by size: 2751 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3378 493 Size: 711 2174 2702 Fragments arranged by size: 2702 2174 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 3776 4411 275 Size: 53 83 186 936 1861 382 635 1451 Fragments arranged by size: 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2127 4020 4418 4692 4839 5250 2127 Size: 1893 398 274 147 411 2464 Fragments arranged by size: 2464 1893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2250 2280 1178 Size: 30 165 30 847 30 4485 Fragments arranged by size: 4485 847 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5587 BseYI C'CCAG_C Cuts at: 4790 5286 4790 Size: 496 5091 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5014 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3648 3733 4804 3648 Size: 85 1071 4431 Fragments arranged by size: 4431 1071 85 BsmI GAATG_Cn' Cuts at: 2879 3098 3191 2879 Size: 219 93 5275 Fragments arranged by size: 5275 219 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2906 3648 3733 4804 1391 Size: 399 246 870 742 85 1071 2174 Fragments arranged by size: 2174 1071 870 742 399 246 85 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 2550 3476 5206 2550 Size: 926 1730 2931 Fragments arranged by size: 2931 1730 926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2942 844 Size: 1547 551 3489 Fragments arranged by size: 3489 1547 551 BsrBI CCG'CTC Cuts at: 1394 1823 3474 1394 Size: 429 1651 3507 Fragments arranged by size: 3507 1651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2127 4231 4418 1700 Size: 110 317 2104 187 2869 Fragments arranged by size: 2869 2104 317 187 110 BsrGI T'GTAC_A Cuts at: 96 2373 96 Size: 2277 3310 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5587 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5587 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstYI r'GATC_y Cuts at: 1972 2058 2152 2563 2608 2917 3284 3669 3686 Size: 86 94 411 45 309 367 385 17 Cuts at: 3686 5138 5224 5236 5582 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 411 385 367 346 309 94 86 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2100 513 Size: 552 972 63 4000 Fragments arranged by size: 4000 972 552 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 2426 3103 3897 3917 2426 Size: 677 794 20 4096 Fragments arranged by size: 4096 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5552 DraI TTT'AAA Cuts at: 2815 2891 2815 Size: 76 5511 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4594 809 Size: 507 3278 1802 Fragments arranged by size: 3278 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 3034 3923 Size: 54 1425 50 275 18 490 714 889 Cuts at: 3923 5552 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 714 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3517 1353 Size: 2164 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4174 4548 4694 387 Size: 1064 138 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 3650 4247 5034 5302 1239 Size: 393 695 1323 597 787 268 1524 Fragments arranged by size: 1524 1323 787 695 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2033 2033 Size: 5587 EcoRI G'AATT_C Cuts at: 2896 3274 2896 Size: 378 5209 FspI TGC'GCA Cuts at: 2019 4093 2019 Size: 2074 3513 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4228 4260 4302 4334 1373 Size: 32 135 32 2656 32 42 32 2626 Fragments arranged by size: 2656 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2545 2931 3177 669 Size: 988 281 607 386 246 3079 Fragments arranged by size: 3079 988 607 386 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4701 4885 1305 Size: 3396 184 2007 Fragments arranged by size: 3396 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2322 10 Size: 54 1425 343 490 3275 Fragments arranged by size: 3275 1425 490 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3545 3904 4063 518 Size: 648 2379 359 159 2042 Fragments arranged by size: 2379 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2039 2046 2401 3003 3680 4828 5073 Size: 417 16 7 355 602 677 1148 245 Cuts at: 5073 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 602 417 355 245 16 7 NcoI C'CATG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4000 Fragments arranged by size: 4000 1035 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2620 2945 4490 1132 Size: 97 1391 325 1545 2229 Fragments arranged by size: 2229 1545 1391 325 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5587 PciI A'CATG_T Cuts at: 2616 4486 2616 Size: 1870 3717 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5587 PfoI T'CCnGG_A Cuts at: 2196 2196 Size: 5587 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3656 3688 5169 5201 3656 Size: 32 1481 32 4042 Fragments arranged by size: 4042 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PspOMI G'GGCC_C Cuts at: 2032 2032 Size: 5587 PspXI vC'TCGA_Gb Cuts at: 2414 2414 Size: 5587 PstI C_TGCA'G Cuts at: 830 2939 830 Size: 2109 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 PvuII CAG'CTG Cuts at: 2023 2401 2023 Size: 378 5209 SacII CC_GC'GG Cuts at: 2040 2040 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2929 1936 Size: 993 4594 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 2282 3835 5547 2282 Size: 1553 1712 2322 Fragments arranged by size: 2322 1712 1553 SfcI C'TryA_G Cuts at: 826 1045 2707 2935 4070 4751 4942 826 Size: 219 1662 228 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 681 228 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2914 1872 Size: 1042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 2650 3691 4592 4854 820 Size: 197 926 471 236 1041 901 262 1553 Fragments arranged by size: 1553 1041 926 901 471 262 236 197 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 2864 3511 5 Size: 47 2812 647 2081 Fragments arranged by size: 2812 2081 647 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5587 StyI C'CwwG_G Cuts at: 513 1065 2100 3026 513 Size: 552 1035 926 3074 Fragments arranged by size: 3074 1035 926 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 3614 3631 3784 3969 4414 325 Size: 1255 657 1377 17 153 185 445 1498 Fragments arranged by size: 1498 1377 1255 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 2373 3833 Size: 274 80 33 51 1443 303 93 1460 Cuts at: 3833 5545 96 Size: 1712 138 Fragments arranged by size: 1712 1460 1443 303 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5547 5579 5547 Size: 32 5555 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2527 2923 2527 Size: 396 5191 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2260 Size: 5587 XhoI C'TCGA_G Cuts at: 1943 2414 1943 Size: 471 5116 XmaI C'CCGG_G Cuts at: 1870 2912 1870 Size: 1042 4545 XmnI GAAnn'nnTTC Cuts at: 2865 3716 2865 Size: 851 4736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI