Restriction map for pFN21AB9540
 (Linear) (Six-base) MAPSORT of: KIBB9540.seq  Check: 7198  from: 1  to: 1053



KIBB9540.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

Acc65I G'GTAC_C

Cuts at:      0     881    1053 
   Size:        881     172
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     587     626    1053 
   Size:        101     486      39     427

  Fragments arranged by size: 

                 486     427     101      39
 
AhdI GACnn_n'nnGTC

Cuts at:      0      48    1053 
   Size:         48    1005
 
AlwNI CAG_nnn'CTG

Cuts at:      0     999    1053 
   Size:        999      54
 
AvrII C'CTAG_G

Cuts at:      0      90    1053 
   Size:         90     963
 
BanI G'GyrC_C

Cuts at:      0     881    1053 
   Size:        881     172
 
BanII G_rGCy'C

Cuts at:      0     172     673     721    1053 
   Size:        172     501      48     332

  Fragments arranged by size: 

                 501     332     172      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     638    1053 
   Size:        638     415
 
BbvCI CC'TCA_GC

Cuts at:      0     695    1053 
   Size:        695     358
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     271     305     442     476    1053 
   Size:        271      34     137      34     577

  Fragments arranged by size: 

                 577     271     137      34      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     133     503     728     812    1053 
   Size:        132       1     370     225      84     241

  Fragments arranged by size: 

                 370     241     225     132      84       1
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     161     695    1053 
   Size:         19     142     534     358

  Fragments arranged by size: 

                 534     358     142      19
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     100     695    1053 
   Size:        100     595     358

  Fragments arranged by size: 

                 595     358     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     979    1009    1053 
   Size:        979      30      44

  Fragments arranged by size: 

                 979      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     162     683    1053 
   Size:        162     521     370

  Fragments arranged by size: 

                 521     370     162
 
BseYI C'CCAG_C

Cuts at:      0     543    1053 
   Size:        543     510
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     673     721    1053 
   Size:        172     501      48     332

  Fragments arranged by size: 

                 501     332     172      48
 
BstYI r'GATC_y

Cuts at:      0     989    1053 
   Size:        989      64
 
BtsI GCAGTG_nn'

Cuts at:      0     555     590    1053 
   Size:        555      35     463

  Fragments arranged by size: 

                 555     463      35
 
EaeI y'GGCC_r

Cuts at:      0     119     423     980    1053 
   Size:        119     304     557      73

  Fragments arranged by size: 

                 557     304     119      73
 
EarI CTCTTCn'nnn_

Cuts at:      0     491     889    1010    1053 
   Size:        491     398     121      43

  Fragments arranged by size: 

                 491     398     121      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     916     985    1053 
   Size:        916      69      68

  Fragments arranged by size: 

                 916      69      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     132     133     503     587     626     728     812 
   Size:        101      31       1     370      84      39     102      84

Cuts at:    812    1053 
   Size:        241

  Fragments arranged by size: 

                 370     241     102     101      84      84      39      31
                   1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     379     711     982    1053 
   Size:        379     332     271      71

  Fragments arranged by size: 

                 379     332     271      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     504    1047    1053 
   Size:        504     543       6

  Fragments arranged by size: 

                 543     504       6
 
EcoRV GAT'ATC

Cuts at:      0     561    1053 
   Size:        561     492
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     220     252    1053 
   Size:         34      32     154      32     801

  Fragments arranged by size: 

                 801     154      34      32      32
 
KpnI G_GTAC'C

Cuts at:      0     885    1053 
   Size:        885     168
 
MfeI C'AATT_G

Cuts at:      0     419    1053 
   Size:        419     634
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     326    1053 
   Size:        236      90     727

  Fragments arranged by size: 

                 727     236      90
 
MscI TGG'CCA

Cuts at:      0     121     425     982    1053 
   Size:        121     304     557      71

  Fragments arranged by size: 

                 557     304     121      71
 
MslI CAynn'nnrTG

Cuts at:      0     664    1053 
   Size:        664     389
 
PasI CC'CwG_GG

Cuts at:      0     499    1053 
   Size:        499     554
 
PfoI T'CCnGG_A

Cuts at:      0     238     380     789    1053 
   Size:        238     142     409     264

  Fragments arranged by size: 

                 409     264     238     142
 
PpuMI rG'GwC_Cy

Cuts at:      0     504    1047    1053 
   Size:        504     543       6

  Fragments arranged by size: 

                 543     504       6
 
SanDI GG'GwC_CC

Cuts at:      0    1047    1053 
   Size:       1047       6
 
SexAI A'CCwGG_T

Cuts at:      0     455    1053 
   Size:        455     598
 
StyI C'CwwG_G

Cuts at:      0      90     983    1053 
   Size:         90     893      70

  Fragments arranged by size: 

                 893      90      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19    1053 
   Size:         19    1034
 
XmnI GAAnn'nnTTC

Cuts at:      0     277    1053 
   Size:        277     776
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI    AlwNI    AvrII     BanI    BanII     BbsI
    BbvCI     BcgI     BpmI   Bpu10I     BsaI    BsaXI    BseRI    BseYI
 Bsp1286I    BstYI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRV    Hin4I     KpnI     MfeI     MmeI     MscI     MslI
     PasI     PfoI    PpuMI    SanDI    SexAI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MluI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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