(Circular) (Six-base) MAPSORT of: pFN21AE0488.seq Check: 7840 from: 1 to: 5812 pFN21AE0488 5812 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 2466 3133 2466 Size: 667 5145 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3875 5259 5527 1632 Size: 2243 1384 268 1917 Fragments arranged by size: 2243 1917 1384 268 AfeI AGC'GCT Cuts at: 2411 2411 Size: 5812 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2319 2808 4711 5688 1203 Size: 1116 489 1903 977 1327 Fragments arranged by size: 1903 1327 1116 977 489 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 2040 4541 2040 Size: 2501 3311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2837 1184 Size: 1653 4159 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2571 2603 2571 Size: 32 5780 AlwNI CAG_nnn'CTG Cuts at: 2380 2733 3555 5127 2380 Size: 353 822 1572 3065 Fragments arranged by size: 3065 1572 822 353 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2090 2498 2544 3137 1870 Size: 73 86 61 408 46 593 4545 Fragments arranged by size: 4545 593 408 86 73 61 46 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2738 2771 2738 Size: 33 5779 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2466 3133 4588 618 Size: 325 217 627 227 452 667 1455 1842 Fragments arranged by size: 1842 1455 667 627 452 325 227 217 BanII G_rGCy'C Cuts at: 1391 2831 2937 3131 1391 Size: 1440 106 194 4072 Fragments arranged by size: 4072 1440 194 106 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 5812 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2497 928 Size: 971 598 4243 Fragments arranged by size: 4243 971 598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 2825 3229 2825 Size: 404 5408 Bme1580I G_kGCm'C Cuts at: 1790 2747 3873 5029 1790 Size: 957 1126 1156 2573 Fragments arranged by size: 2573 1156 1126 957 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2058 2932 3543 Size: 416 53 83 399 24 641 874 611 Cuts at: 3543 4501 442 Size: 958 1753 Fragments arranged by size: 1753 958 874 641 611 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2128 4472 1239 Size: 889 2344 2579 Fragments arranged by size: 2579 2344 889 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2606 4475 882 Size: 617 1107 1869 2219 Fragments arranged by size: 2219 1869 1107 617 BsaAI yAC'GTr Cuts at: 493 1204 2236 2320 3603 493 Size: 711 1032 84 1283 2702 Fragments arranged by size: 2702 1283 1032 711 84 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 3619 4001 4636 Size: 53 83 186 936 482 1604 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1604 1451 936 635 482 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4245 4643 4917 5064 5475 4245 Size: 398 274 147 411 4582 Fragments arranged by size: 4582 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5812 BseYI C'CCAG_C Cuts at: 2675 2943 5015 5511 2675 Size: 268 2072 496 2976 Fragments arranged by size: 2976 2072 496 268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 1163 Size: 573 599 4640 Fragments arranged by size: 4640 599 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3072 3262 4023 5051 1540 Size: 275 186 1071 190 761 1028 2301 Fragments arranged by size: 2301 1071 1028 761 275 190 186 BsiHKAI G_wGCw'C Cuts at: 2853 3873 3958 5029 2853 Size: 1020 85 1071 3636 Fragments arranged by size: 3636 1071 1020 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 BsmBI CGTCTCn'nnnn_ Cuts at: 2269 2269 Size: 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2747 2831 2853 2937 3131 3873 3958 Size: 399 957 84 22 84 194 742 85 Cuts at: 3958 5029 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 957 742 399 194 85 84 84 22 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2400 2529 3167 844 Size: 1556 129 638 3489 Fragments arranged by size: 3489 1556 638 129 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 2463 2478 4456 4643 1700 Size: 110 337 316 15 1978 187 2869 Fragments arranged by size: 2869 1978 337 316 187 110 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2880 794 Size: 2086 3726 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstYI r'GATC_y Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807 Size: 1170 367 385 17 1452 86 12 346 Cuts at: 5807 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2129 2129 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 2004 2747 3023 513 Size: 552 939 743 276 3302 Fragments arranged by size: 3302 939 743 552 276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2125 487 Size: 683 56 899 4174 Fragments arranged by size: 4174 899 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3069 3259 4148 Size: 54 1425 50 275 18 1239 190 889 Cuts at: 4148 5777 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1239 889 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3069 3259 1537 Size: 275 1257 190 4090 Fragments arranged by size: 4090 1257 275 190 EarI CTCTTCn'nnn_ Cuts at: 1353 2219 3742 1353 Size: 866 1523 3423 Fragments arranged by size: 3423 1523 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2128 3875 4472 5259 5527 1239 Size: 393 496 1747 597 787 268 1524 Fragments arranged by size: 1747 1524 787 597 496 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2282 2282 Size: 5812 EcoO109I rG'GnC_Cy Cuts at: 2024 2365 2024 Size: 341 5471 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 2018 2413 2959 4959 1678 Size: 340 395 546 2000 2531 Fragments arranged by size: 2531 2000 546 395 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 4453 4485 4527 Size: 32 135 32 454 32 2395 32 42 Cuts at: 4527 4559 1373 Size: 32 2626 Fragments arranged by size: 2626 2395 454 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2272 3156 3402 669 Size: 988 281 334 884 246 3079 Fragments arranged by size: 3079 988 884 334 281 246 HindIII A'AGCT_T Cuts at: 748 2625 748 Size: 1877 3935 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KasI G'GCGC_C Cuts at: 2014 2014 Size: 5812 KpnI G_GTAC'C Cuts at: 2470 3137 2470 Size: 667 5145 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 2808 5688 2808 Size: 2880 2932 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2819 3770 4129 4288 518 Size: 648 1026 627 951 359 159 2042 Fragments arranged by size: 2042 1026 951 648 627 359 159 MspA1I CmG'CkG Cuts at: 1606 2183 2679 3068 3228 3905 5053 5298 1606 Size: 577 496 389 160 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 577 496 389 245 160 NaeI GCC'GGC Cuts at: 2480 2480 Size: 5812 NarI GG'CG_CC Cuts at: 2015 2015 Size: 5812 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4321 Fragments arranged by size: 4321 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NgoMIV G'CCGG_C Cuts at: 2478 2478 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 2990 3170 4715 1132 Size: 97 1761 180 1545 2229 Fragments arranged by size: 2229 1761 1545 180 97 PasI CC'CwG_GG Cuts at: 1348 2831 1348 Size: 1483 4329 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4910 PfoI T'CCnGG_A Cuts at: 3103 3103 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PmlI CAC'GTG Cuts at: 2236 2236 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5394 5426 3881 Size: 32 1481 32 4267 Fragments arranged by size: 4267 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2024 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PspXI vC'TCGA_Gb Cuts at: 2090 2090 Size: 5812 PstI C_TGCA'G Cuts at: 830 2310 2397 2642 3164 830 Size: 1480 87 245 522 3478 Fragments arranged by size: 3478 1480 522 245 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SanDI GG'GwC_CC Cuts at: 2024 2024 Size: 5812 SbfI CC_TGCA'GG Cuts at: 2397 3164 2397 Size: 767 5045 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2306 2393 2638 2734 3160 4295 4976 Size: 219 1261 87 245 96 426 1135 681 Cuts at: 4976 5167 826 Size: 191 1471 Fragments arranged by size: 1471 1261 1135 681 426 245 219 191 96 87 SfoI GGC'GCC Cuts at: 2016 2016 Size: 5812 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 2546 3139 1872 Size: 674 593 4545 Fragments arranged by size: 4545 674 593 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 3916 4817 5079 820 Size: 197 926 147 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 147 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 2990 3170 1132 Size: 1858 180 3774 Fragments arranged by size: 3774 1858 180 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2004 2019 3251 513 Size: 552 939 15 1232 3074 Fragments arranged by size: 3074 1232 939 552 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 3839 3856 4009 4194 4639 325 Size: 1255 789 1470 17 153 185 445 1498 Fragments arranged by size: 1498 1470 1255 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 4058 5770 Size: 274 80 33 51 1443 345 1736 1712 Cuts at: 5770 96 Size: 138 Fragments arranged by size: 1736 1712 1443 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 2090 1943 Size: 147 5665 XmaI C'CCGG_G Cuts at: 1870 2544 3137 1870 Size: 674 593 4545 Fragments arranged by size: 4545 674 593 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI