(Circular) (Six-base) MAPSORT of: pFN21AB6382.seq Check: 4596 from: 1 to: 5605 pFN21AB6382 5605 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 2482 3668 5052 5320 1632 Size: 557 293 1186 1384 268 1917 Fragments arranged by size: 1917 1384 1186 557 293 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2739 2795 4504 5481 1203 Size: 1536 56 1709 977 1327 Fragments arranged by size: 1709 1536 1327 977 56 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 3348 4920 3348 Size: 1572 4033 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2189 2914 3140 3292 5401 1493 Size: 696 725 226 152 2109 1697 Fragments arranged by size: 2109 1697 725 696 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2712 2930 1870 Size: 73 769 218 4545 Fragments arranged by size: 4545 769 218 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2924 1391 Size: 1533 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2488 2894 928 Size: 971 589 406 3639 Fragments arranged by size: 3639 971 589 406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2267 3497 4707 2267 Size: 1230 1210 3165 Fragments arranged by size: 3165 1230 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 3666 4822 1790 Size: 1876 1156 2573 Fragments arranged by size: 2573 1876 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2645 4265 1239 Size: 797 609 1620 2579 Fragments arranged by size: 2579 1620 797 609 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 2810 3730 4595 4893 2039 Size: 771 920 865 298 2751 Fragments arranged by size: 2751 920 865 771 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2447 4268 882 Size: 617 948 1821 2219 Fragments arranged by size: 2219 1821 948 617 BsaAI yAC'GTr Cuts at: 493 1204 2798 3396 493 Size: 711 1594 598 2702 Fragments arranged by size: 2702 1594 711 598 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 3412 3794 4429 Size: 53 83 186 936 1110 769 382 635 Cuts at: 4429 275 Size: 1451 Fragments arranged by size: 1451 1110 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 4038 4436 4710 4857 5268 2086 Size: 1952 398 274 147 411 2423 Fragments arranged by size: 2423 1952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2610 1186 Size: 868 556 4181 Fragments arranged by size: 4181 868 556 BseYI C'CCAG_C Cuts at: 4808 5304 4808 Size: 496 5109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2741 1163 Size: 573 1005 4027 Fragments arranged by size: 4027 1005 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3055 3816 4844 1540 Size: 275 186 72 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 2713 2777 3666 3751 4822 2713 Size: 64 889 85 1071 3496 Fragments arranged by size: 3496 1071 889 85 64 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 2036 Size: 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2713 2777 2924 3666 3751 4822 1391 Size: 399 923 64 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 923 742 399 147 85 64 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2763 2960 844 Size: 1919 197 3489 Fragments arranged by size: 3489 1919 197 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 2216 794 Size: 1422 4183 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600 Size: 963 367 385 17 1452 86 12 346 Cuts at: 5600 1972 Size: 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5053 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2216 2216 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2528 4612 809 Size: 507 1212 2084 1802 Fragments arranged by size: 2084 1802 1212 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 3052 3941 Size: 54 1425 50 275 18 240 982 889 Cuts at: 3941 5570 8 Size: 1629 43 Fragments arranged by size: 1629 1425 982 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2070 3052 1537 Size: 275 258 982 4090 Fragments arranged by size: 4090 982 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 3535 1353 Size: 1044 1138 3423 Fragments arranged by size: 3423 1138 1044 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2189 2482 2645 3668 4265 5052 Size: 393 404 153 293 163 1023 597 787 Cuts at: 5052 5320 1239 Size: 268 1524 Fragments arranged by size: 1524 1023 787 597 404 393 293 268 163 153 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 5605 EcoO109I rG'GnC_Cy Cuts at: 2125 2596 2125 Size: 471 5134 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 4246 4278 4320 Size: 32 135 32 693 32 1949 32 42 Cuts at: 4320 4352 1373 Size: 32 2626 Fragments arranged by size: 2626 1949 693 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 4903 1305 Size: 3414 184 2007 Fragments arranged by size: 3414 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3783 Fragments arranged by size: 3783 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 2798 3563 3922 4081 518 Size: 648 1534 98 765 359 159 2042 Fragments arranged by size: 2042 1534 765 648 359 159 98 MspA1I CmG'CkG Cuts at: 1606 2648 3021 3698 4846 5091 1606 Size: 1042 373 677 1148 245 2120 Fragments arranged by size: 2120 1148 1042 677 373 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5053 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 2070 3052 2070 Size: 982 4623 NspI r_CATG'y Cuts at: 1132 1229 2350 2963 4508 1132 Size: 97 1121 613 1545 2229 Fragments arranged by size: 2229 1545 1121 613 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2745 2745 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2596 2125 Size: 471 5134 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2957 830 Size: 2127 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2953 4088 4769 4960 826 Size: 219 1908 1135 681 191 1471 Fragments arranged by size: 1908 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 2712 2825 3709 4610 4872 Size: 197 926 111 658 113 884 901 262 Cuts at: 4872 820 Size: 1553 Fragments arranged by size: 1553 926 901 884 658 262 197 113 111 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 2783 1517 Size: 1266 4339 StyI C'CwwG_G Cuts at: 513 1065 2319 3044 513 Size: 552 1254 725 3074 Fragments arranged by size: 3074 1254 725 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 3632 3649 3802 3987 4432 325 Size: 1255 1062 990 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1062 990 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 2483 1316 Size: 1167 4438 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 2712 1943 Size: 769 4836 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 2307 3734 2307 Size: 1427 4178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI