################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstF5I BstNI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI ItaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI TseI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 274 GTAGAC GT^[AC][GT]AC AluI 344 AGCT AG^CT AvaI 44 CTCGGG C^[CT]CG[GA]G AvaII 447 GGACC G^G[AT]CC BanI 487 GGCACC G^G[CT][GA]CC BpmI 231 CTGGAG CTGGAG(16/14) BsaI 290 GAGACC GGTCTC(1/5) BsmBI 160 GAGACG CGTCTC(1/5) BsoBI 44 CTCGGG C^[CT]CG[GA]G Bsp1407I 101 TGTACA T^GTACA BspMI 435 ACCTGC ACCTGC(4/8) BsrDI 505 GCAATG GCAATG(2/0) BsrFI 490 ACCGGC [GA]^CCGG[CT] BsrGI 101 TGTACA T^GTACA BstEII 431 GGTTACC G^GT[ACGT]ACC BstPI 431 GGTTACC G^GT[ACGT]ACC BstXI 356 CCATTTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 490 ACCGGC [GA]^CCGG[CT] Cfr13I 447 GGACC G^G[ACGT]CC EarI 340 GAAGAG CTCTTC(1/4) EcoNI 495 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 431 GGTTACC G^GT[ACGT]ACC HapII 491 CCGG C^CGG HpaII 491 CCGG C^CGG HphI 165 GGTGA GGTGA(8/7) Ksp632I 340 GAAGAG CTCTTC(1/4) MslI 357 CATTTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 491 CCGG C^CGG NlaIV 487 GGCACC GG[ACGT]^[ACGT]CC SapI 340 GAAGAGC GCTCTTC(1/4) Sau96I 447 GGACC G^G[ACGT]CC SfaNI 256 GATGC GCATC(5/9) SmlI 15 CTCAAG C^T[CT][GA]AG SspBI 101 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 102 GTAC GT^AC 171 GTAC BsmAI 160 GAGAC GTCTC(1/5) 290 GAGAC BsrI 230 ACTGG ACTGG(1/-1) 29 CCAGT DdeI 346 CTGAG C^T[ACGT]AG 408 CTCAG MaeII 169 ACGT A^CGT 472 ACGT MboII 337 GAAGA GAAGA(8/7) 340 GAAGA RsaI 102 GTAC GT^AC 171 GTAC Tsp45I 166 GTGAC ^GT[GC]AC 284 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AcsI ApoI BsiYI BslI HaeIII MaeIII MnlI MseI NlaIII Tru9I Tsp509I