Restriction map for
pFN21AB6880
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MwoI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI TspRI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 503 CGCG CG^CG
AcsI 329 AAATTC [GA]^AATT[CT]
AflIII 24 ACATGT A^C[GA][CT]GT
AlwI 181 GATCC GGATC(4/5)
AocI 448 CCTGAGG CC^T[ACGT]AGG
ApoI 329 AAATTC [GA]^AATT[CT]
AvaI 237 CCCGGG C^[CT]CG[GA]G
BbvCI 558 CCTCAGC CCTCAGC(-5/-2)
BglII 267 AGATCT A^GATCT
BmyI 233 GTGCCC G[AGT]GC[ACT]^C
BsaAI 510 CACGTA [CT]AC^GT[GA]
BsoBI 237 CCCGGG C^[CT]CG[GA]G
Bsp1286I 233 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 24 ACATGT A^CATGT
BstF5I 36 GGATG GGATG(2/0)
BstNI 219 CCTGG CC^[AT]GG
BstUI 503 CGCG CG^CG
Bsu36I 448 CCTGAGG CC^T[ACGT]AGG
CfoI 502 GCGC GCG^C
DsaI 251 CCATGG C^C[GA][CT]GG
Eco81I 448 CCTGAGG CC^T[ACGT]AGG
EcoNI 279 CCTCAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 219 CCTGG ^CC[AT]GG
FokI 36 GGATG GGATG(9/13)
HhaI 502 GCGC GCG^C
HinP1I 502 GCGC G^CGC
MboII 530 TCTTC GAAGA(8/7)
MspA1I 561 CAGCTG C[AC]G^C[GT]G
MvaI 219 CCTGG CC^[AT]GG
MvnI 503 CGCG CG^CG
NcoI 251 CCATGG C^CATGG
PstI 103 CTGCAG CTGCA^G
PvuII 561 CAGCTG CAG^CTG
SmaI 237 CCCGGG CCC^GGG
SphI 494 GCATGC GCATG^C
StuI 363 AGGCCT AGG^CCT
TaqI 580 TCGA T^CGA
TthHB8I 580 TCGA T^CGA
XcmI 488 CCAAGGGCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 237 CCCGGG C^CCGGG
XmaI 237 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 141 GTAC GT^AC
513 GTAC
BpmI 470 CTCCAG CTGGAG(16/14)
263 CTGGAG
BsiYI 279 CCTCAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTCAGCTGGG
BslI 279 CCTCAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTCAGCTGGG
BsmFI 240 GGGAC GGGAC(10/14)
90 GTCCC
BstYI 180 AGATCC [GA]^GATC[CT]
267 AGATCT
DdeI 449 CTGAG C^T[ACGT]AG
559 CTCAG
DpnII 181 GATC ^GATC
268 GATC
EcoT14I 251 CCATGG C^C[AT][AT]GG
488 CCAAGG
HaeIII 364 GGCC GG^CC
446 GGCC
HapII 210 CCGG C^CGG
238 CCGG
HinfI 129 GAGTC G^A[ACGT]TC
323 GAGTC
HpaII 210 CCGG C^CGG
238 CCGG
MaeII 374 ACGT A^CGT
511 ACGT
MaeIII 175 GTGAC ^GT[ACGT]AC
205 GTCAC
MboI 181 GATC ^GATC
268 GATC
MflI 180 AGATCC [GA]^GATC[CT]
267 AGATCT
MspI 210 CCGG C^CGG
238 CCGG
NdeII 181 GATC ^GATC
268 GATC
NspI 24 ACATGT [GA]CATG^[CT]
494 GCATGC
PleI 129 GAGTC GAGTC(4/5)
323 GAGTC
RsaI 141 GTAC GT^AC
513 GTAC
Sau3AI 181 GATC ^GATC
268 GATC
SfcI 103 CTGCAG C^T[GA][CT]AG
387 CTACAG
StyI 251 CCATGG C^C[AT][AT]GG
488 CCAAGG
Tsp45I 175 GTGAC ^GT[GC]AC
205 GTCAC
XhoII 180 AGATCC [GA]^GATC[CT]
267 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AvaII BbvI BcnI BsaJI
BseRI BsrI BssKI Cac8I Cfr13I
Fnu4HI HphI ItaI MnlI MseI
NciI NlaIII NlaIV Sau96I ScrFI
Tru9I TseI Tsp509I