Restriction map for
pFN21AB6880
(Linear) (Six-base) MAPSORT of: KIBB6880.seq Check: 513 from: 1 to: 603
KIBB6880.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AflIII A'CryG_T
Cuts at: 0 24 603
Size: 24 579
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 445 477 603
Size: 445 32 126
Fragments arranged by size:
445 126 32
ApoI r'AATT_y
Cuts at: 0 329 603
Size: 329 274
AvaI C'yCGr_G
Cuts at: 0 237 603
Size: 237 366
BbvCI CC'TCA_GC
Cuts at: 0 559 603
Size: 559 44
BglII A'GATC_T
Cuts at: 0 267 603
Size: 267 336
Bme1580I G_kGCm'C
Cuts at: 0 237 603
Size: 237 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 404 603
Size: 404 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 455 603
Size: 284 171 148
Fragments arranged by size:
284 171 148
Bpu10I CC'TnA_GC
Cuts at: 0 559 603
Size: 559 44
BsaAI yAC'GTr
Cuts at: 0 512 603
Size: 512 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 603
Size: 445 30 128
Fragments arranged by size:
445 128 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 466 493 603
Size: 46 420 27 110
Fragments arranged by size:
420 110 46 27
BseYI C'CCAG_C
Cuts at: 0 156 563 603
Size: 156 407 40
Fragments arranged by size:
407 156 40
Bsp1286I G_dGCh'C
Cuts at: 0 237 603
Size: 237 366
BstYI r'GATC_y
Cuts at: 0 180 267 603
Size: 180 87 336
Fragments arranged by size:
336 180 87
Bsu36I CC'TnA_GG
Cuts at: 0 449 603
Size: 449 154
BtgI C'CryG_G
Cuts at: 0 251 603
Size: 251 352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 455 603
Size: 284 171 148
Fragments arranged by size:
284 171 148
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 603
Size: 283 320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 546 603
Size: 546 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 603
Size: 343 32 228
Fragments arranged by size:
343 228 32
MspA1I CmG'CkG
Cuts at: 0 563 603
Size: 563 40
NcoI C'CATG_G
Cuts at: 0 251 603
Size: 251 352
NspI r_CATG'y
Cuts at: 0 28 498 603
Size: 28 470 105
Fragments arranged by size:
470 105 28
PciI A'CATG_T
Cuts at: 0 24 603
Size: 24 579
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 603
Size: 445 32 126
Fragments arranged by size:
445 126 32
PsiI TTA'TAA
Cuts at: 0 71 603
Size: 71 532
PstI C_TGCA'G
Cuts at: 0 107 603
Size: 107 496
PvuII CAG'CTG
Cuts at: 0 563 603
Size: 563 40
SfcI C'TryA_G
Cuts at: 0 103 387 603
Size: 103 284 216
Fragments arranged by size:
284 216 103
SmaI CCC'GGG
Cuts at: 0 239 603
Size: 239 364
SphI G_CATG'C
Cuts at: 0 498 603
Size: 498 105
StuI AGG'CCT
Cuts at: 0 365 603
Size: 365 238
StyI C'CwwG_G
Cuts at: 0 251 488 603
Size: 251 237 115
Fragments arranged by size:
251 237 115
TatI w'GTAC_w
Cuts at: 0 140 603
Size: 140 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 603
Size: 508 32 63
Fragments arranged by size:
508 63 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 603
Size: 495 108
XmaI C'CCGG_G
Cuts at: 0 237 603
Size: 237 366
Enzymes that do cut:
AflIII AloI ApoI AvaI BbvCI BglII Bme1580I BmrI
BpmI Bpu10I BsaAI BsaXI BseRI BseYI Bsp1286I BstYI
Bsu36I BtgI Eco57MI EcoNI FalI Hin4I MspA1I NcoI
NspI PciI PpiI PsiI PstI PvuII SfcI SmaI
SphI StuI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI XmnI ZraI