(Circular) (Six-base) MAPSORT of: pFN21AB6345.seq Check: 8044 from: 1 to: 5500 pFN21AB6345 5500 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3310 278 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2843 1937 Size: 906 4594 Acc65I G'GTAC_C Cuts at: 2821 2821 Size: 5500 AclI AA'CG_TT Cuts at: 1563 3627 4000 1563 Size: 2064 373 3063 Fragments arranged by size: 3063 2064 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3563 4947 5215 1632 Size: 1931 1384 268 1917 Fragments arranged by size: 1931 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5303 AflIII A'CryG_T Cuts at: 1203 4399 5376 1203 Size: 3196 977 1327 Fragments arranged by size: 3196 1327 977 AgeI A'CCGG_T Cuts at: 4331 4331 Size: 5500 AhdI GACnn_n'nnGTC Cuts at: 4229 4229 Size: 5500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5500 AlwNI CAG_nnn'CTG Cuts at: 2182 3243 4815 2182 Size: 1061 1572 2867 Fragments arranged by size: 2867 1572 1061 ApaI G_GGCC'C Cuts at: 2634 2634 Size: 5500 ApaLI G'TGCA_C Cuts at: 3557 4713 3557 Size: 1156 4344 ApoI r'AATT_y Cuts at: 1493 2809 3035 3187 5296 1493 Size: 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 AseI AT'TA_AT Cuts at: 160 1032 4054 160 Size: 872 3022 1606 Fragments arranged by size: 3022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 AvaI C'yCGr_G Cuts at: 1870 1943 2343 2825 1870 Size: 73 400 482 4545 Fragments arranged by size: 4545 482 400 73 BamHI G'GATC_C Cuts at: 2830 3197 2830 Size: 367 5133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2294 2329 2821 4276 618 Size: 325 217 627 507 35 492 1455 1842 Fragments arranged by size: 1842 1455 627 507 492 325 217 35 BanII G_rGCy'C Cuts at: 1391 2096 2483 2634 2819 1391 Size: 705 387 151 185 4072 Fragments arranged by size: 4072 705 387 185 151 BbeI G_GCGC'C Cuts at: 2298 2298 Size: 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 2783 928 Size: 971 172 712 3645 Fragments arranged by size: 3645 971 712 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3691 3725 1903 Size: 34 1754 34 3678 Fragments arranged by size: 3678 1754 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3392 4602 3392 Size: 1210 4290 BclI T'GATC_A Cuts at: 1548 1548 Size: 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2478 4111 136 Size: 107 122 71 631 605 806 1633 1525 Fragments arranged by size: 1633 1525 806 631 605 122 107 71 BglII A'GATC_T Cuts at: 2247 5495 2247 Size: 3248 2252 BlpI GC'TnA_GC Cuts at: 2917 2917 Size: 5500 Bme1580I G_kGCm'C Cuts at: 1790 2634 3561 4717 1790 Size: 844 927 1156 2573 Fragments arranged by size: 2573 1156 927 844 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 2758 3231 Size: 416 53 83 399 24 1206 135 473 Cuts at: 3231 4189 442 Size: 958 1753 Fragments arranged by size: 1753 1206 958 473 416 399 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2038 2038 Size: 5500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4160 1239 Size: 2921 2579 Bpu10I CC'TnA_GC Cuts at: 2693 2693 Size: 5500 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2021 2087 3625 4490 4788 2021 Size: 66 1538 865 298 2733 Fragments arranged by size: 2733 1538 865 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4163 882 Size: 617 2664 2219 Fragments arranged by size: 2664 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2275 2730 3291 493 Size: 711 1071 455 561 2702 Fragments arranged by size: 2702 1071 711 561 455 BsaBI GATnn'nnATC Cuts at: 5494 5494 Size: 5500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2295 3307 3689 4324 Size: 53 83 186 936 762 1012 382 635 Cuts at: 4324 275 Size: 1451 Fragments arranged by size: 1451 1012 936 762 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3933 4331 4605 4752 5163 3933 Size: 398 274 147 411 4270 Fragments arranged by size: 4270 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5275 Fragments arranged by size: 5275 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2768 1186 Size: 1582 3918 BseYI C'CCAG_C Cuts at: 2283 2485 2560 4703 5199 2283 Size: 202 75 2143 496 2584 Fragments arranged by size: 2584 2143 496 202 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4927 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2950 3711 4739 1540 Size: 275 186 949 761 1028 2301 Fragments arranged by size: 2301 1028 949 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 3561 3646 4717 2024 Size: 1537 85 1071 2807 Fragments arranged by size: 2807 1537 1071 85 BsmI GAATG_Cn' Cuts at: 3011 3104 3011 Size: 93 5407 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2096 2483 2634 2819 3561 3646 Size: 399 234 72 387 151 185 742 85 Cuts at: 3646 4717 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 234 185 151 85 72 BspEI T'CCGG_A Cuts at: 5163 5163 Size: 5500 BspHI T'CATG_A Cuts at: 2589 3389 5119 2589 Size: 800 1730 2970 Fragments arranged by size: 2970 1730 800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2682 2855 844 Size: 1838 173 3489 Fragments arranged by size: 3489 1838 173 BsrBI CCG'CTC Cuts at: 1394 1823 2196 2335 2568 2609 2770 3387 1394 Size: 429 373 139 233 41 161 617 3507 Fragments arranged by size: 3507 617 429 373 233 161 139 41 BsrDI GCAATG_nn' Cuts at: 65 1248 2298 3995 4169 65 Size: 1183 1050 1697 174 1396 Fragments arranged by size: 1697 1396 1183 1050 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4144 4331 1700 Size: 110 2334 187 2869 Fragments arranged by size: 2869 2334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5500 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5500 BssSI C'ACGA_G Cuts at: 3560 4572 3560 Size: 1012 4488 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5500 BstBI TT'CG_AA Cuts at: 4304 4304 Size: 5500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5500 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2404 2476 2224 Size: 180 72 5248 Fragments arranged by size: 5248 180 72 BstYI r'GATC_y Cuts at: 1972 2247 2830 3197 3582 3599 5051 5137 5149 Size: 275 583 367 385 17 1452 86 12 Cuts at: 5149 5495 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 275 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2397 2781 513 Size: 552 960 372 384 3232 Fragments arranged by size: 3232 960 552 384 372 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4761 Fragments arranged by size: 4761 683 56 BtsI GCAGTG_nn' Cuts at: 3016 3810 3830 3016 Size: 794 20 4686 Fragments arranged by size: 4686 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5465 DraI TTT'AAA Cuts at: 2804 2804 Size: 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4507 809 Size: 507 3191 1802 Fragments arranged by size: 3191 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2108 2157 Size: 54 1425 50 275 18 231 47 49 Cuts at: 2157 2200 2355 2712 2947 3836 5465 8 Size: 43 155 357 235 889 1629 43 Fragments arranged by size: 1629 1425 889 357 275 235 231 155 54 50 49 47 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2947 1537 Size: 275 1135 4090 Fragments arranged by size: 4090 1135 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2684 2760 3430 1353 Size: 1331 76 670 3423 Fragments arranged by size: 3423 1331 670 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4087 4461 4607 387 Size: 1064 138 2498 374 146 1280 Fragments arranged by size: 2498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3563 4160 4947 5215 1239 Size: 393 1931 597 787 268 1524 Fragments arranged by size: 1931 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2179 2186 2630 2179 Size: 7 444 5049 Fragments arranged by size: 5049 444 7 EcoRI G'AATT_C Cuts at: 2809 3187 2809 Size: 378 5122 FspI TGC'GCA Cuts at: 4006 4006 Size: 5500 HaeII r_GCGC'y Cuts at: 1678 2120 2298 4647 1678 Size: 442 178 2349 2531 Fragments arranged by size: 2531 2349 442 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4141 4173 4215 4247 1373 Size: 32 135 32 2569 32 42 32 2626 Fragments arranged by size: 2626 2569 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 2844 3090 669 Size: 988 281 424 482 246 3079 Fragments arranged by size: 3079 988 482 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5500 HpaI GTT'AAC Cuts at: 3090 3090 Size: 5500 KasI G'GCGC_C Cuts at: 2294 2294 Size: 5500 KpnI G_GTAC'C Cuts at: 2825 2825 Size: 5500 MfeI C'AATT_G Cuts at: 3099 3099 Size: 5500 MluI A'CGCG_T Cuts at: 5376 5376 Size: 5500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2509 4614 4798 1305 Size: 1204 2105 184 2007 Fragments arranged by size: 2105 2007 1204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2063 2159 2714 10 Size: 54 1425 343 231 96 555 2796 Fragments arranged by size: 2796 1425 555 343 231 96 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 3458 3817 3976 518 Size: 648 1236 1056 359 159 2042 Fragments arranged by size: 2042 1236 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2287 2428 2761 2916 3593 4741 4986 1606 Size: 681 141 333 155 677 1148 245 2120 Fragments arranged by size: 2120 1148 681 677 333 245 155 141 NarI GG'CG_CC Cuts at: 2295 2295 Size: 5500 NcoI C'CATG_G Cuts at: 513 1065 2397 2781 513 Size: 552 1332 384 3232 Fragments arranged by size: 3232 1332 552 384 NdeI CA'TA_TG Cuts at: 387 387 Size: 5500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5500 NotI GC'GGCC_GC Cuts at: 2947 2947 Size: 5500 NspI r_CATG'y Cuts at: 1132 1229 2858 4403 1132 Size: 97 1629 1545 2229 Fragments arranged by size: 2229 1629 1545 97 PasI CC'CwG_GG Cuts at: 1348 2182 2515 1348 Size: 834 333 4333 Fragments arranged by size: 4333 834 333 PciI A'CATG_T Cuts at: 4399 4399 Size: 5500 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 2290 2398 1108 Size: 1074 108 108 4210 Fragments arranged by size: 4210 1074 108 108 PmeI GTTT'AAAC Cuts at: 2804 2804 Size: 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 5082 5114 3569 Size: 32 1481 32 3955 Fragments arranged by size: 3955 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2186 Size: 5500 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5500 PsiI TTA'TAA Cuts at: 3070 3070 Size: 5500 PspOMI G'GGCC_C Cuts at: 2630 2630 Size: 5500 PstI C_TGCA'G Cuts at: 830 2136 2852 830 Size: 1306 716 3478 Fragments arranged by size: 3478 1306 716 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5500 PvuII CAG'CTG Cuts at: 2287 2428 2287 Size: 141 5359 SalI G'TCGA_C Cuts at: 1936 2842 1936 Size: 906 4594 SanDI GG'GwC_CC Cuts at: 2186 2186 Size: 5500 SapI GCTCTTCn'nnn_ Cuts at: 2684 2760 2684 Size: 76 5424 SbfI CC_TGCA'GG Cuts at: 2852 2852 Size: 5500 ScaI AGT'ACT Cuts at: 3748 5460 3748 Size: 1712 3788 SfcI C'TryA_G Cuts at: 826 1045 2132 2848 3983 4664 4855 826 Size: 219 1087 716 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 716 681 219 191 SfoI GGC'GCC Cuts at: 2296 2296 Size: 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5500 SmaI CCC'GGG Cuts at: 1872 2345 2827 1872 Size: 473 482 4545 Fragments arranged by size: 4545 482 473 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2102 3604 4505 4767 820 Size: 197 926 93 66 1502 901 262 1553 Fragments arranged by size: 1553 1502 926 901 262 197 93 66 SnaBI TAC'GTA Cuts at: 493 3291 493 Size: 2798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5500 SphI G_CATG'C Cuts at: 1132 2858 1132 Size: 1726 3774 SspI AAT'ATT Cuts at: 5 52 3424 5 Size: 47 3372 2081 Fragments arranged by size: 3372 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5500 StyI C'CwwG_G Cuts at: 513 1065 2397 2781 2939 513 Size: 552 1332 384 158 3074 Fragments arranged by size: 3074 1332 552 384 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3527 3544 3697 3882 4327 325 Size: 1255 1947 17 153 185 445 1498 Fragments arranged by size: 1947 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3746 5458 96 Size: 274 80 33 51 1443 1769 1712 138 Fragments arranged by size: 1769 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 5468 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5500 XbaI T'CTAG_A Cuts at: 2836 2836 Size: 5500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2167 Size: 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5500 XmaI C'CCGG_G Cuts at: 1870 2343 2825 1870 Size: 473 482 4545 Fragments arranged by size: 4545 482 473 XmnI GAAnn'nnTTC Cuts at: 2597 3629 2597 Size: 1032 4468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3308 276 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI