Restriction map for pFN21AB6453
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsgI        BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        DsaI        Eam1105I    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MunI        MvnI        NaeI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          481  GAATTT               [GA]^AATT[CT]
AcyI          839  GGCGCC               G[GA]^CG[CT]C
AlwNI         325  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       557  AGCGCT               AGC^GCT
ApaI          120  GGGCCC               GGGCC^C
ApoI          481  GAATTT               [GA]^AATT[CT]
Asp700I       568  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          46  GAGCTC               G[AT]GC[AT]^C
AvaII         138  GGACC                G^G[AT]CC
BbeI          839  GGCGCC               GGCGC^C
BbsI           34  GAAGAC               GAAGAC(2/6)
BcnI          465  CCGGG                CC^[GC]GG
BfaI          946  CTAG                 C^TAG
BglII         188  AGATCT               A^GATCT
BpmI          544  CTGGAG               CTGGAG(16/14)
BpuAI          34  GAAGAC               GAAGAC(2/6)
BsaHI         839  GGCGCC               G[GA]^CG[CT]C
BsiEI          83  CGGCCG               CG[GA][CT]^CG
BsiHKAI        46  GAGCTC               G[AT]GC[AT]^C
BsmFI         861  GTCCC                GGGAC(10/14)
BsrBI          23  GAGCGG               CCGCTC(-3/-3)
BsrFI         551  ACCGGC               [GA]^CCGG[CT]
BstAPI        732  GCAGGCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        551  ACCGGC               [GA]^CCGG[CT]
DdeI           49  CTCAG                C^T[ACGT]AG
DraIII        161  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           83  CGGCCG               [CT]^GGCC[GA]
EagI           83  CGGCCG               C^GGCCG
EarI           72  GAAGAG               CTCTTC(1/4)
EclXI          83  CGGCCG               C^GGCCG
Eco47III      557  AGCGCT               AGC^GCT
Eco52I         83  CGGCCG               C^GGCCG
FauI          404  CCCGC                CCCGC(4/6)
Hin1I         839  GGCGCC               G[GA]^CG[CT]C
KasI          839  GGCGCC               G^GCGCC
Ksp632I        72  GAAGAG               CTCTTC(1/4)
MaeI          946  CTAG                 C^TAG
MseI          227  TTAA                 T^TAA
MspA1I        314  CAGCTG               C[AC]G^C[GT]G
NarI          839  GGCGCC               GG^CGCC
NciI          465  CCGGG                CC^[GC]GG
NdeI          509  CATATG               CA^TATG
PpuMI         137  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          730  CTGCAG               CTGCA^G
PvuII         314  CAGCTG               CAG^CTG
SacI           46  GAGCTC               GAGCT^C
SfcI          730  CTGCAG               C^T[GA][CT]AG
SfoI          839  GGCGCC               GGC^GCC
SmlI          184  CTCAAG               C^T[CT][GA]AG
Tru9I         227  TTAA                 T^TAA
Tsp45I        887  GTGAC                ^GT[GC]AC
XmnI          568  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          821  GATCC                GGATC(4/5)
              897  GATCC               
BanI          761  GGCACC               G^G[CT][GA]CC
              839  GGCGCC              
BanII          46  GAGCTC               G[GA]GC[CT]^C
              120  GGGCCC              
BclI          453  TGATCA               T^GATCA
              921  TGATCA              
BsaI          286  GAGACC               GGTCTC(1/5)
              626  GAGACC              
BseRI         442  GAGGAG               GAGGAG(10/8)
              445  GAGGAG              
BsmAI         286  GAGAC                GTCTC(1/5)
              626  GAGAC               
BsmI          684  GAATGC               GAATGC(1/-1)
              813  GAATGC              
BspMI         174  ACCTGC               ACCTGC(4/8)
              876  ACCTGC              
BstYI         188  AGATCT               [GA]^GATC[CT]
              896  AGATCC              
EcoNI         129  CCTGTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              517  CCTAATGCAGG         
FbaI          453  TGATCA               T^GATCA
              921  TGATCA              
HapII         465  CCGG                 C^CGG
              552  CCGG                
HinfI         338  GACTC                G^A[ACGT]TC
              487  GACTC               
HpaII         465  CCGG                 C^CGG
              552  CCGG                
MflI          188  AGATCT               [GA]^GATC[CT]
              896  AGATCC              
MspI          465  CCGG                 C^CGG
              552  CCGG                
NlaIII        451  CATG                 CATG^
              643  CATG                
PleI          338  GACTC                GAGTC(4/5)
              487  GACTC               
TaqI          216  TCGA                 T^CGA
              819  TCGA                
TthHB8I       216  TCGA                 T^CGA
              819  TCGA                
XhoII         188  AGATCT               [GA]^GATC[CT]
              896  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HhaI        HinP1I      HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       StyI        TseI        Tsp509I     TspRI       
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