(Circular) (Six-base) MAPSORT of: pFN21AB6453.seq Check: 4791 from: 1 to: 5659 pFN21AB6453 5659 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 2612 3722 5106 5374 1632 Size: 680 300 1110 1384 268 1917 Fragments arranged by size: 1917 1384 1110 680 300 268 AfeI AGC'GCT Cuts at: 2564 2564 Size: 5659 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2335 3402 4974 2335 Size: 1067 1572 3020 Fragments arranged by size: 3020 1572 1067 ApaI G_GGCC'C Cuts at: 2129 2129 Size: 5659 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2486 2968 3194 3346 5455 1493 Size: 993 482 226 152 2109 1697 Fragments arranged by size: 2109 1697 993 482 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2537 2570 2537 Size: 33 5626 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2766 2844 2980 4435 618 Size: 325 217 627 979 78 136 1455 1842 Fragments arranged by size: 1842 1455 979 627 325 217 136 78 BanII G_rGCy'C Cuts at: 1391 2055 2129 2978 1391 Size: 664 74 849 4072 Fragments arranged by size: 4072 849 664 74 BbeI G_GCGC'C Cuts at: 2848 2848 Size: 5659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 928 Size: 971 147 4541 Fragments arranged by size: 4541 971 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3850 3884 1903 Size: 34 50 34 1829 34 3678 Fragments arranged by size: 3678 1829 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2458 2926 1548 Size: 910 468 4281 Fragments arranged by size: 4281 910 468 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2193 5654 2193 Size: 3461 2198 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2129 2420 3720 4876 1790 Size: 339 291 1300 1156 2573 Fragments arranged by size: 2573 1300 1156 339 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2163 3390 4348 Size: 416 53 83 399 24 746 1227 958 Cuts at: 4348 442 Size: 1753 Fragments arranged by size: 1753 1227 958 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 4319 1239 Size: 1331 1749 2579 Fragments arranged by size: 2579 1749 1331 Bpu10I CC'TnA_GC Cuts at: 2054 2054 Size: 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 3784 4649 4947 2174 Size: 1610 865 298 2886 Fragments arranged by size: 2886 1610 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2285 2625 4322 882 Size: 617 786 340 1697 2219 Fragments arranged by size: 2219 1697 786 617 340 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2845 3466 3848 4483 Size: 53 83 186 936 1312 621 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1312 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 2465 1186 Size: 1276 3 4380 Fragments arranged by size: 4380 1276 3 BseYI C'CCAG_C Cuts at: 2128 2565 4862 5358 2128 Size: 437 2297 496 2429 Fragments arranged by size: 2429 2297 496 437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5086 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2091 3109 3870 4898 1540 Size: 275 186 90 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 90 BsiHKAI G_wGCw'C Cuts at: 2055 3720 3805 4876 2055 Size: 1665 85 1071 2838 Fragments arranged by size: 2838 1665 1071 85 BsmI GAATG_Cn' Cuts at: 2695 2824 3170 3263 2695 Size: 129 346 93 5091 Fragments arranged by size: 5091 346 129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2129 2420 2978 3720 3805 4876 Size: 399 265 74 291 558 742 85 1071 Cuts at: 4876 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 558 399 291 265 85 74 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2188 2890 3014 844 Size: 1344 702 124 3489 Fragments arranged by size: 3489 1344 702 124 BsrBI CCG'CTC Cuts at: 1394 1823 2030 3546 1394 Size: 429 207 1516 3507 Fragments arranged by size: 3507 1516 429 207 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 4303 4490 1700 Size: 110 746 1747 187 2869 Fragments arranged by size: 2869 1747 746 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 2743 794 Size: 1949 3710 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2193 2901 2989 3356 3741 3758 5210 5296 Size: 221 708 88 367 385 17 1452 86 Cuts at: 5296 5308 5654 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 708 385 367 346 221 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3175 3969 3989 2929 Size: 246 794 20 4599 Fragments arranged by size: 4599 794 246 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DraIII CAC_nnn'GTG Cuts at: 2171 2171 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 3106 3995 Size: 54 1425 50 275 18 258 1018 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1018 889 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2088 3106 1537 Size: 275 276 1018 4090 Fragments arranged by size: 4090 1018 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3589 1353 Size: 719 1517 3423 Fragments arranged by size: 3423 1517 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 4246 4620 4766 387 Size: 1064 138 462 2195 374 146 1280 Fragments arranged by size: 2195 1280 1064 462 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2053 Size: 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2570 2612 3722 4319 5106 5374 Size: 393 680 258 42 1110 597 787 268 Cuts at: 5374 1239 Size: 1524 Fragments arranged by size: 1524 1110 787 680 597 393 268 258 42 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2526 2138 Size: 388 5271 EcoO109I rG'GnC_Cy Cuts at: 2125 2143 2596 2914 2125 Size: 18 453 318 4870 Fragments arranged by size: 4870 453 318 18 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2566 2716 2848 4806 1678 Size: 888 150 132 1958 2531 Fragments arranged by size: 2531 1958 888 150 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2806 2838 4300 4332 4374 Size: 32 135 32 1234 32 1462 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 1462 1234 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KasI G'GCGC_C Cuts at: 2844 2844 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 3976 4135 518 Size: 648 2451 359 159 2042 Fragments arranged by size: 2451 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 3075 3752 4900 5145 1606 Size: 715 754 677 1148 245 2120 Fragments arranged by size: 2120 1148 754 715 677 245 NarI GG'CG_CC Cuts at: 2845 2845 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5107 NdeI CA'TA_TG Cuts at: 387 2515 387 Size: 2128 3531 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2143 Size: 5659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2125 2125 Size: 5659 PstI C_TGCA'G Cuts at: 830 2739 3011 830 Size: 1909 272 3478 Fragments arranged by size: 3478 1909 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2321 2321 Size: 5659 SacI G_AGCT'C Cuts at: 2055 2055 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2735 3007 4142 4823 5014 826 Size: 219 1690 272 1135 681 191 1471 Fragments arranged by size: 1690 1471 1135 681 272 219 191 SfoI GGC'GCC Cuts at: 2846 2846 Size: 5659 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3763 4664 4926 820 Size: 197 926 246 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2673 2716 2910 3098 513 Size: 552 1608 43 194 188 3074 Fragments arranged by size: 3074 1608 552 194 188 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2182 3686 3703 3856 4041 4486 325 Size: 1255 602 1504 17 153 185 445 1498 Fragments arranged by size: 1504 1498 1255 602 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 2577 3788 2577 Size: 1211 4448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI