################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AccII AccIII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BcnI BlnI BsaWI BseAI BseRI BsiEI BsiWI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FbaI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 2798 GACGTC GACGT^C AclI 3574 AACGTT AA^CGTT AcyI 2798 GACGTC G[GA]^CG[CT]C Alw44I 950 GTGCAC G^TGCAC AocI 821 CCTTAGG CC^T[ACGT]AGG ApaLI 950 GTGCAC G^TGCAC AspI 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1439 TGGCCA TGG^CCA BanI 1149 GGTGCC G^G[CT][GA]CC BanII 136 GAGCCC G[GA]GC[CT]^C BbrPI 3637 CACGTG CAC^GTG BglI 3341 GCCACAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1705 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1705 GCTAAGC GC^T[ACGT]AGC BsaBI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2798 GACGTC G[GA]^CG[CT]C BsgI 1580 GTGCAG GTGCAG(16/14) BsmBI 643 CGTCTC CGTCTC(1/5) Bsp1407I 1656 TGTACA T^GTACA BspMI 1987 ACCTGC ACCTGC(4/8) BsrDI 2892 CATTGC GCAATG(2/0) BsrGI 1656 TGTACA T^GTACA BstAPI 2530 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 414 GGTCACC G^GT[ACGT]ACC BstPI 414 GGTCACC G^GT[ACGT]ACC Bsu36I 821 CCTTAGG CC^T[ACGT]AGG CelII 1705 GCTAAGC GC^T[ACGT]AGC CfoI 3357 GCGC GCG^C DraI 1614 TTTAAA TTT^AAA DsaI 3492 CCATGG C^C[GA][CT]GG EaeI 1439 TGGCCA [CT]^GGCC[GA] Eco81I 821 CCTTAGG CC^T[ACGT]AGG EcoNI 1131 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 414 GGTCACC G^GT[ACGT]ACC EcoRI 2019 GAATTC G^AATTC EcoRV 1364 GATATC GAT^ATC FauI 1787 CCCGC CCCGC(4/6) HapII 1678 CCGG C^CGG HhaI 3357 GCGC GCG^C Hin1I 2798 GACGTC G[GA]^CG[CT]C HinP1I 3357 GCGC G^CGC HincII 2785 GTTGAC GT[CT]^[GA]AC HindII 2785 GTTGAC GT[CT]^[GA]AC HpaII 1678 CCGG C^CGG MamI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1439 TGGCCA TGG^CCA MscI 1439 TGGCCA TGG^CCA MspA1I 2252 CAGCTG C[AC]G^C[GT]G MspI 1678 CCGG C^CGG NcoI 3492 CCATGG C^CATGG NdeI 3659 CATATG CA^TATG PflFI 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3637 CACGTG CAC^GTG PmlI 3637 CACGTG CAC^GTG Psp1406I 3574 AACGTT AA^CGTT PvuII 2252 CAGCTG CAG^CTG SnaBI 202 TACGTA TAC^GTA SspBI 1656 TGTACA T^GTACA Tth111I 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 419 CCATACATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflII 2871 CTTAAG C^TTAAG 3625 CTTAAG AflIII 3636 ACACGT A^C[GA][CT]GT 3638 ACGTGT AseI 2308 ATTAAT AT^TAAT 2812 ATTAAT AsnI 2308 ATTAAT AT^TAAT 2812 ATTAAT Asp700I 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2316 GAAACAGTTC BfrI 2871 CTTAAG C^TTAAG 3625 CTTAAG BsaI 677 GGTCTC GGTCTC(1/5) 1666 GGTCTC EcoT14I 673 CCAAGG C^C[AT][AT]GG 3492 CCATGG MfeI 2073 CAATTG C^AATTG 3064 CAATTG MunI 2073 CAATTG C^AATTG 3064 CAATTG PpuMI 789 GGGTCCT [GA]G^G[AT]CC[CT] 2339 AGGACCC PshBI 2308 ATTAAT AT^TAAT 2812 ATTAAT SpeI 2037 ACTAGT A^CTAGT 2449 ACTAGT StyI 673 CCAAGG C^C[AT][AT]GG 3492 CCATGG XbaI 1056 TCTAGA T^CTAGA 1917 TCTAGA XmnI 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2316 GAAACAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII