Restriction map for
pFN21AE0348
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DsaI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StyI SwaI Tru9I Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 1020 GAATTT [GA]^AATT[CT]
Alw44I 1099 GTGCAC G^TGCAC
AocI 175 CCTGAGG CC^T[ACGT]AGG
Aor51HI 884 AGCGCT AGC^GCT
ApaI 1455 GGGCCC GGGCC^C
ApaLI 1099 GTGCAC G^TGCAC
ApoI 1020 GAATTT [GA]^AATT[CT]
Asp700I 948 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 592 CTCGAG C^[CT]CG[GA]G
BbeI 1253 GGCGCC GGCGC^C
BbvCI 648 CCTCAGC CCTCAGC(-5/-2)
BcgI 1164 CGAGGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmFI 188 GGGAC GGGAC(10/14)
BsoBI 592 CTCGAG C^[CT]CG[GA]G
BsrDI 1088 GCAATG GCAATG(2/0)
BssHII 587 GCGCGC G^CGCGC
BssSI 1162 CACGAG CACGAG(-5/-1)
Bsu36I 175 CCTGAGG CC^T[ACGT]AGG
DrdI 507 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 884 AGCGCT AGC^GCT
Eco81I 175 CCTGAGG CC^T[ACGT]AGG
EcoNI 747 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1253 GGCGCC G^GCGCC
MaeIII 959 GTGAC ^GT[ACGT]AC
MboII 303 GAAGA GAAGA(8/7)
NarI 1253 GGCGCC GG^CGCC
NheI 12 GCTAGC G^CTAGC
PaeR7I 592 CTCGAG C^TCGAG
PflMI 777 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1312 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 750 CCTGCAGG CCTGCA^GG
SexAI 800 ACCTGGT A^CC[AT]GGT
SfoI 1253 GGCGCC GGC^GCC
Sse8387I 750 CCTGCAGG CCTGCA^GG
StuI 1166 AGGCCT AGG^CCT
Tsp45I 959 GTGAC ^GT[GC]AC
Tsp509I 1021 AATT ^AATT
Van91I 777 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 592 CTCGAG C^TCGAG
XmnI 948 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 910 GTCTAC GT^[AC][GT]AC
1393 GTCTAC
AlwI 435 GATCC GGATC(4/5)
879 GATCC
AspHI 1075 GAGCAC G[AT]GC[AT]^C
1099 GTGCAC
BalI 218 TGGCCA TGG^CCA
638 TGGCCA
BanII 157 GAGCCC G[GA]GC[CT]^C
1455 GGGCCC
BcnI 169 CCGGG CC^[GC]GG
831 CCCGG
BseRI 34 CTCCTC GAGGAG(10/8)
631 GAGGAG
BsgI 229 CTGCAC GTGCAG(16/14)
872 GTGCAG
BsiHKAI 1075 GAGCAC G[AT]GC[AT]^C
1099 GTGCAC
BstYI 434 AGATCC [GA]^GATC[CT]
878 AGATCC
HapII 169 CCGG C^CGG
832 CCGG
HgaI 1363 GACGC GACGC(5/10)
1391 GCGTC
HpaII 169 CCGG C^CGG
832 CCGG
MflI 434 AGATCC [GA]^GATC[CT]
878 AGATCC
MluNI 218 TGGCCA TGG^CCA
638 TGGCCA
MscI 218 TGGCCA TGG^CCA
638 TGGCCA
MslI 54 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
918 CATCCAGGTG
MspI 169 CCGG C^CGG
832 CCGG
NciI 169 CCGGG CC^[GC]GG
831 CCCGG
PleI 190 GACTC GAGTC(4/5)
1300 GACTC
SmlI 333 CTTGAG C^T[CT][GA]AG
592 CTCGAG
TfiI 211 GAATC G^A[AT]TC
375 GAATC
XhoII 434 AGATCC [GA]^GATC[CT]
878 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
Hin1I HinP1I HinfI HphI ItaI
MaeI MboI MnlI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I XcmI