Restriction map for
pFN21AE0348
(Linear) (Six-base) MAPSORT of: KIEE0348.seq Check: 5270 from: 1 to: 1509
KIEE0348.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 246 838 846 971 1509
Size: 246 592 8 125 538
Fragments arranged by size:
592 538 246 125 8
AccI GT'mk_AC
Cuts at: 0 911 1394 1509
Size: 911 483 115
Fragments arranged by size:
911 483 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 556 1509
Size: 322 234 953
Fragments arranged by size:
953 322 234
AfeI AGC'GCT
Cuts at: 0 886 1509
Size: 886 623
AlwNI CAG_nnn'CTG
Cuts at: 0 279 300 468 543 612 783 1509
Size: 279 21 168 75 69 171 726
Fragments arranged by size:
726 279 171 168 75 69 21
ApaI G_GGCC'C
Cuts at: 0 1459 1509
Size: 1459 50
ApaLI G'TGCA_C
Cuts at: 0 1099 1509
Size: 1099 410
ApoI r'AATT_y
Cuts at: 0 1020 1509
Size: 1020 489
AvaI C'yCGr_G
Cuts at: 0 592 1509
Size: 592 917
BanI G'GyrC_C
Cuts at: 0 403 797 1012 1253 1509
Size: 403 394 215 241 256
Fragments arranged by size:
403 394 256 241 215
BanII G_rGCy'C
Cuts at: 0 161 1459 1509
Size: 161 1298 50
Fragments arranged by size:
1298 161 50
BbeI G_GCGC'C
Cuts at: 0 1257 1509
Size: 1257 252
BbvCI CC'TCA_GC
Cuts at: 0 649 1509
Size: 649 860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1153 1187 1509
Size: 1153 34 322
Fragments arranged by size:
1153 322 34
BlpI GC'TnA_GC
Cuts at: 0 461 679 1245 1509
Size: 461 218 566 264
Fragments arranged by size:
566 461 264 218
Bme1580I G_kGCm'C
Cuts at: 0 406 1103 1335 1459 1509
Size: 406 697 232 124 50
Fragments arranged by size:
697 406 232 124 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 1161 1170 1509
Size: 1161 9 339
Fragments arranged by size:
1161 339 9
BmtI G_CTAG'C
Cuts at: 0 16 1509
Size: 16 1493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 187 289 367 547 550 634 726
Size: 168 19 102 78 180 3 84 92
Cuts at: 726 1367 1509
Size: 641 142
Fragments arranged by size:
641 180 168 142 102 92 84 78
19 3
Bpu10I CC'TnA_GC
Cuts at: 0 649 1509
Size: 649 860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1509
Size: 354 1155
BsaI GGTCTCn'nnnn_
Cuts at: 0 186 499 610 1055 1509
Size: 186 313 111 445 454
Fragments arranged by size:
454 445 313 186 111
BsaHI Gr'CG_yC
Cuts at: 0 1254 1364 1391 1509
Size: 1254 110 27 118
Fragments arranged by size:
1254 118 110 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 646 1509
Size: 25 621 863
Fragments arranged by size:
863 621 25
BseYI C'CCAG_C
Cuts at: 0 8 251 399 1065 1069 1083 1459 1509
Size: 8 243 148 666 4 14 376 50
Fragments arranged by size:
666 376 243 148 50 14 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 893 1509
Size: 214 679 616
Fragments arranged by size:
679 616 214
BsiHKAI G_wGCw'C
Cuts at: 0 1079 1103 1509
Size: 1079 24 406
Fragments arranged by size:
1079 406 24
Bsp1286I G_dGCh'C
Cuts at: 0 161 406 1079 1103 1335 1459 1509
Size: 161 245 673 24 232 124 50
Fragments arranged by size:
673 245 232 161 124 50 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 342 838 846 971 1509
Size: 246 96 496 8 125 538
Fragments arranged by size:
538 496 246 125 96 8
BsrDI GCAATG_nn'
Cuts at: 0 1095 1509
Size: 1095 414
BssHII G'CGCG_C
Cuts at: 0 587 1509
Size: 587 922
BssSI C'ACGA_G
Cuts at: 0 1162 1509
Size: 1162 347
BstAPI GCAn_nnn'nTGC
Cuts at: 0 279 771 804 1509
Size: 279 492 33 705
Fragments arranged by size:
705 492 279 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 528 889 1509
Size: 60 468 361 620
Fragments arranged by size:
620 468 361 60
BstYI r'GATC_y
Cuts at: 0 434 878 1509
Size: 434 444 631
Fragments arranged by size:
631 444 434
Bsu36I CC'TnA_GG
Cuts at: 0 176 1509
Size: 176 1333
BtsI GCAGTG_nn'
Cuts at: 0 226 277 301 425 978 1206 1468 1509
Size: 226 51 24 124 553 228 262 41
Fragments arranged by size:
553 262 228 226 124 51 41 24
DraIII CAC_nnn'GTG
Cuts at: 0 296 804 1474 1509
Size: 296 508 670 35
Fragments arranged by size:
670 508 296 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 513 1509
Size: 513 996
EaeI y'GGCC_r
Cuts at: 0 120 218 638 1509
Size: 120 98 420 871
Fragments arranged by size:
871 420 120 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 1509
Size: 100 1409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 187 289 322 367 547 550 556
Size: 168 19 102 33 45 180 3 6
Cuts at: 556 634 726 1367 1509
Size: 78 92 641 142
Fragments arranged by size:
641 180 168 142 102 92 78 45
33 19 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 751 1509
Size: 751 758
EcoO109I rG'GnC_Cy
Cuts at: 0 172 609 1455 1456 1509
Size: 172 437 846 1 53
Fragments arranged by size:
846 437 172 53 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 348 1509
Size: 348 1161
HaeII r_GCGC'y
Cuts at: 0 695 888 1002 1257 1509
Size: 695 193 114 255 252
Fragments arranged by size:
695 255 252 193 114
KasI G'GCGC_C
Cuts at: 0 1253 1509
Size: 1253 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1509
Size: 218 1291
MscI TGG'CCA
Cuts at: 0 220 640 1509
Size: 220 420 869
Fragments arranged by size:
869 420 220
MslI CAynn'nnrTG
Cuts at: 0 58 922 1509
Size: 58 864 587
Fragments arranged by size:
864 587 58
MspA1I CmG'CkG
Cuts at: 0 267 540 780 1029 1069 1241 1439 1509
Size: 267 273 240 249 40 172 198 70
Fragments arranged by size:
273 267 249 240 198 172 70 40
NarI GG'CG_CC
Cuts at: 0 1254 1509
Size: 1254 255
NheI G'CTAG_C
Cuts at: 0 12 1509
Size: 12 1497
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 1509
Size: 783 726
PfoI T'CCnGG_A
Cuts at: 0 863 1509
Size: 863 646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 1509
Size: 844 32 633
Fragments arranged by size:
844 633 32
PshAI GACnn'nnGTC
Cuts at: 0 1316 1509
Size: 1316 193
PspOMI G'GGCC_C
Cuts at: 0 1455 1509
Size: 1455 54
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1198 1230 1509
Size: 1198 32 279
Fragments arranged by size:
1198 279 32
PstI C_TGCA'G
Cuts at: 0 26 284 755 968 1277 1509
Size: 26 258 471 213 309 232
Fragments arranged by size:
471 309 258 232 213 26
PvuII CAG'CTG
Cuts at: 0 267 540 780 1069 1509
Size: 267 273 240 289 440
Fragments arranged by size:
440 289 273 267 240
SbfI CC_TGCA'GG
Cuts at: 0 755 1509
Size: 755 754
SexAI A'CCwGG_T
Cuts at: 0 800 1509
Size: 800 709
SfcI C'TryA_G
Cuts at: 0 22 126 280 547 751 964 1273 1509
Size: 22 104 154 267 204 213 309 236
Fragments arranged by size:
309 267 236 213 204 154 104 22
SfoI GGC'GCC
Cuts at: 0 1255 1509
Size: 1255 254
SmlI C'TyrA_G
Cuts at: 0 333 592 1509
Size: 333 259 917
Fragments arranged by size:
917 333 259
StuI AGG'CCT
Cuts at: 0 1168 1509
Size: 1168 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 463 1509
Size: 265 198 1046
Fragments arranged by size:
1046 265 198
TatI w'GTAC_w
Cuts at: 0 1418 1509
Size: 1418 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 1509
Size: 95 32 1382
Fragments arranged by size:
1382 95 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 396 525 1342 1509
Size: 57 339 129 817 167
Fragments arranged by size:
817 339 167 129 57
XhoI C'TCGA_G
Cuts at: 0 592 1509
Size: 592 917
XmnI GAAnn'nnTTC
Cuts at: 0 952 1509
Size: 952 557
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbvCI BcgI BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtsI DraIII DrdI EaeI
EciI Eco57MI EcoNI EcoO109I FalI HaeII KasI MmeI
MscI MslI MspA1I NarI NheI PflMI PfoI PpiI
PshAI PspOMI PsrI PstI PvuII SbfI SexAI SfcI
SfoI SmlI StuI TaqII TatI TstI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BglI BglII BmgBI BplI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BstBI BstEII BstZ17I
BtgI BtgZI ClaI CspCI DraI EagI EarI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpuMI PsiI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I
XbaI XmaI ZraI