(Circular) (Six-base) MAPSORT of: pFN21AE0327.seq Check: 7578 from: 1 to: 6559 pFN21AE0327 6559 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4369 278 Size: 53 83 186 936 2833 2468 Fragments arranged by size: 2833 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 3902 1937 Size: 1851 114 4594 Fragments arranged by size: 4594 1851 114 Acc65I G'GTAC_C Cuts at: 3880 3880 Size: 6559 AclI AA'CG_TT Cuts at: 1563 4686 5059 1563 Size: 3123 373 3063 Fragments arranged by size: 3123 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 2193 3158 3470 4622 6006 6274 1632 Size: 422 139 965 312 1152 1384 268 1917 Fragments arranged by size: 1917 1384 1152 965 422 312 268 139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6362 AflIII A'CryG_T Cuts at: 1203 5458 6435 1203 Size: 4255 977 1327 Fragments arranged by size: 4255 1327 977 AgeI A'CCGG_T Cuts at: 5390 5390 Size: 6559 AhdI GACnn_n'nnGTC Cuts at: 5288 5288 Size: 6559 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6559 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6559 AlwNI CAG_nnn'CTG Cuts at: 2032 4302 5874 2032 Size: 2270 1572 2717 Fragments arranged by size: 2717 2270 1572 ApaI G_GGCC'C Cuts at: 3342 3342 Size: 6559 ApaLI G'TGCA_C Cuts at: 4616 5772 4616 Size: 1156 5403 ApoI r'AATT_y Cuts at: 1493 2391 2941 3028 3359 3868 4094 4246 6355 Size: 898 550 87 331 509 226 152 2109 Cuts at: 6355 1493 Size: 1697 Fragments arranged by size: 2109 1697 898 550 509 331 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 2928 5113 160 Size: 872 1896 2185 1606 Fragments arranged by size: 2185 1896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 AvaI C'yCGr_G Cuts at: 1870 1943 3663 3884 1870 Size: 73 1720 221 4545 Fragments arranged by size: 4545 1720 221 73 BamHI G'GATC_C Cuts at: 3889 4256 3889 Size: 367 6192 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 2794 3880 5335 618 Size: 325 217 627 380 627 1086 1455 1842 Fragments arranged by size: 1842 1455 1086 627 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 2023 3294 3342 3878 1391 Size: 632 1271 48 536 4072 Fragments arranged by size: 4072 1271 632 536 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2429 3271 928 Size: 971 530 842 4216 Fragments arranged by size: 4216 971 842 530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2495 2529 4750 4784 1903 Size: 34 558 34 2221 34 3678 Fragments arranged by size: 3678 2221 558 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2053 3141 3363 4451 5661 2053 Size: 1088 222 1088 1210 2951 Fragments arranged by size: 2951 1210 1088 1088 222 BclI T'GATC_A Cuts at: 1548 1548 Size: 6559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 5170 136 Size: 107 122 71 631 605 365 3133 1525 Fragments arranged by size: 3133 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 3737 6554 3737 Size: 2817 3742 BlpI GC'TnA_GC Cuts at: 3976 3976 Size: 6559 Bme1580I G_kGCm'C Cuts at: 1790 2170 3342 4620 5776 1790 Size: 380 1172 1278 1156 2573 Fragments arranged by size: 2573 1278 1172 1156 380 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6559 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 4290 5248 Size: 416 53 83 399 24 858 2015 958 Cuts at: 5248 442 Size: 1753 Fragments arranged by size: 2015 1753 958 858 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2528 1056 Size: 1472 5087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3254 5219 1239 Size: 2015 1965 2579 Fragments arranged by size: 2579 2015 1965 Bpu10I CC'TnA_GC Cuts at: 2228 2228 Size: 6559 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4684 5549 5847 4684 Size: 865 298 5396 Fragments arranged by size: 5396 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5222 882 Size: 617 3723 2219 Fragments arranged by size: 3723 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2509 3799 4350 493 Size: 711 1305 1290 551 2702 Fragments arranged by size: 2702 1305 1290 711 551 BsaBI GATnn'nnATC Cuts at: 3351 6553 3351 Size: 3202 3357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4366 4748 5383 275 Size: 53 83 186 936 2833 382 635 1451 Fragments arranged by size: 2833 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2127 4992 5390 5664 5811 6222 2127 Size: 2865 398 274 147 411 2464 Fragments arranged by size: 2865 2464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3043 3073 1178 Size: 30 165 30 1640 30 4664 Fragments arranged by size: 4664 1640 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2868 3122 1186 Size: 1682 254 4623 Fragments arranged by size: 4623 1682 254 BseYI C'CCAG_C Cuts at: 2095 2323 2653 2666 3452 5762 6258 2095 Size: 228 330 13 786 2310 496 2396 Fragments arranged by size: 2396 2310 786 496 330 228 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2715 3153 1163 Size: 573 979 438 4569 Fragments arranged by size: 4569 979 573 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4009 4770 5798 1540 Size: 275 186 2008 761 1028 2301 Fragments arranged by size: 2301 2008 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 3240 4620 4705 5776 2127 Size: 1113 1380 85 1071 2910 Fragments arranged by size: 2910 1380 1113 1071 85 BsmI GAATG_Cn' Cuts at: 2570 3758 4070 4163 2570 Size: 1188 312 93 4966 Fragments arranged by size: 4966 1188 312 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2127 2170 3240 3294 3342 3878 Size: 399 233 104 43 1070 54 48 536 Cuts at: 3878 4620 4705 5776 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1070 742 536 399 233 104 85 54 48 43 BspEI T'CCGG_A Cuts at: 6222 6222 Size: 6559 BspHI T'CATG_A Cuts at: 2733 4448 6178 2733 Size: 1715 1730 3114 Fragments arranged by size: 3114 1730 1715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3012 3914 844 Size: 2168 902 3489 Fragments arranged by size: 3489 2168 902 BsrBI CCG'CTC Cuts at: 1394 1823 4446 1394 Size: 429 2623 3507 Fragments arranged by size: 3507 2623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2712 5054 5228 65 Size: 1183 1395 69 2342 174 1396 Fragments arranged by size: 2342 1396 1395 1183 174 69 BsrFI r'CCGG_y Cuts at: 1700 1810 5203 5390 1700 Size: 110 3393 187 2869 Fragments arranged by size: 3393 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2595 96 Size: 2499 4060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6559 BssSI C'ACGA_G Cuts at: 4619 5631 4619 Size: 1012 5547 BstAPI GCAn_nnn'nTGC Cuts at: 794 2384 2556 794 Size: 1590 172 4797 Fragments arranged by size: 4797 1590 172 BstBI TT'CG_AA Cuts at: 5363 5363 Size: 6559 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6559 BstYI r'GATC_y Cuts at: 1972 2177 3737 3889 4256 4641 4658 6110 6196 Size: 205 1560 152 367 385 17 1452 86 Cuts at: 6196 6208 6554 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1560 1452 385 367 346 205 152 86 17 12 BstZ17I GTA'TAC Cuts at: 3789 3789 Size: 6559 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5068 Fragments arranged by size: 5068 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2446 487 Size: 683 56 1220 4600 Fragments arranged by size: 4600 1220 683 56 BtsI GCAGTG_nn' Cuts at: 2059 4075 4869 4889 2059 Size: 2016 794 20 3729 Fragments arranged by size: 3729 2016 794 20 ClaI AT'CG_AT Cuts at: 1554 3445 1554 Size: 1891 4668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3026 3061 3538 3573 529 Size: 35 2462 35 477 35 3515 Fragments arranged by size: 3515 2462 477 35 35 35 DraI TTT'AAA Cuts at: 3863 3863 Size: 6559 DraIII CAC_nnn'GTG Cuts at: 3214 3214 Size: 6559 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5566 809 Size: 507 4250 1802 Fragments arranged by size: 4250 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2369 2684 3546 Size: 54 1425 50 275 18 539 315 862 Cuts at: 3546 4006 4895 6524 8 Size: 460 889 1629 43 Fragments arranged by size: 1629 1425 889 862 539 460 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4006 1537 Size: 275 2194 4090 Fragments arranged by size: 4090 2194 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2744 3585 4489 1353 Size: 1391 841 904 3423 Fragments arranged by size: 3423 1391 904 841 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5146 5520 5666 387 Size: 1064 138 3557 374 146 1280 Fragments arranged by size: 3557 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2193 3158 3254 3470 4622 5219 Size: 393 422 139 965 96 216 1152 597 Cuts at: 5219 6006 6274 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1152 965 787 597 422 393 268 216 139 96 EcoO109I rG'GnC_Cy Cuts at: 3339 3539 3339 Size: 200 6359 EcoRI G'AATT_C Cuts at: 2391 3868 4246 2391 Size: 1477 378 4704 Fragments arranged by size: 4704 1477 378 EcoRV GAT'ATC Cuts at: 3227 3332 3613 3227 Size: 105 281 6173 Fragments arranged by size: 6173 281 105 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2773 2773 Size: 6559 FspI TGC'GCA Cuts at: 5065 5065 Size: 6559 HaeII r_GCGC'y Cuts at: 1678 2032 3156 5706 1678 Size: 354 1124 2550 2531 Fragments arranged by size: 2550 2531 1124 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5200 5232 5274 5306 1373 Size: 32 135 32 3628 32 42 32 2626 Fragments arranged by size: 3628 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3903 4149 669 Size: 988 281 1965 246 3079 Fragments arranged by size: 3079 1965 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6559 HpaI GTT'AAC Cuts at: 4149 4149 Size: 6559 KpnI G_GTAC'C Cuts at: 3884 3884 Size: 6559 MfeI C'AATT_G Cuts at: 3475 4158 3475 Size: 683 5876 MluI A'CGCG_T Cuts at: 6435 6435 Size: 6559 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5673 5857 1305 Size: 4368 184 2007 Fragments arranged by size: 4368 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2371 2686 3548 10 Size: 54 1425 343 539 315 862 3021 Fragments arranged by size: 3021 1425 862 539 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 2648 3139 4517 4876 5035 518 Size: 648 1383 99 491 1378 359 159 2042 Fragments arranged by size: 2042 1383 1378 648 491 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2043 2375 2666 3975 4652 5800 6045 1606 Size: 437 332 291 1309 677 1148 245 2120 Fragments arranged by size: 2120 1309 1148 677 437 332 291 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5068 Fragments arranged by size: 5068 939 552 NdeI CA'TA_TG Cuts at: 387 2329 387 Size: 1942 4617 NheI G'CTAG_C Cuts at: 1052 2524 1052 Size: 1472 5087 NotI GC'GGCC_GC Cuts at: 4006 4006 Size: 6559 NspI r_CATG'y Cuts at: 1132 1229 2554 3917 5462 1132 Size: 97 1325 1363 1545 2229 Fragments arranged by size: 2229 1545 1363 1325 97 PacI TTA_AT'TAA Cuts at: 2928 2928 Size: 6559 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6559 PciI A'CATG_T Cuts at: 5458 5458 Size: 6559 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6559 PmeI GTTT'AAAC Cuts at: 3863 3863 Size: 6559 PmlI CAC'GTG Cuts at: 2509 2509 Size: 6559 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4628 4660 6141 6173 4628 Size: 32 1481 32 5014 Fragments arranged by size: 5014 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3539 3539 Size: 6559 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6559 PsiI TTA'TAA Cuts at: 3080 4129 3080 Size: 1049 5510 PspOMI G'GGCC_C Cuts at: 3338 3338 Size: 6559 PstI C_TGCA'G Cuts at: 830 3012 3911 830 Size: 2182 899 3478 Fragments arranged by size: 3478 2182 899 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6559 PvuII CAG'CTG Cuts at: 2375 2666 2375 Size: 291 6268 RsrII CG'GwC_CG Cuts at: 2130 2130 Size: 6559 SalI G'TCGA_C Cuts at: 1936 3901 1936 Size: 1965 4594 SapI GCTCTTCn'nnn_ Cuts at: 3585 3585 Size: 6559 SbfI CC_TGCA'GG Cuts at: 3911 3911 Size: 6559 ScaI AGT'ACT Cuts at: 4807 6519 4807 Size: 1712 4847 SfcI C'TryA_G Cuts at: 826 1045 3008 3375 3712 3907 5042 5723 5914 Size: 219 1963 367 337 195 1135 681 191 Cuts at: 5914 826 Size: 1471 Fragments arranged by size: 1963 1471 1135 681 367 337 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6559 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6559 SmaI CCC'GGG Cuts at: 1872 3665 3886 1872 Size: 1793 221 4545 Fragments arranged by size: 4545 1793 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 4663 5564 5826 820 Size: 197 926 2720 901 262 1553 Fragments arranged by size: 2720 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3799 4350 493 Size: 3306 551 2702 Fragments arranged by size: 3306 2702 551 SpeI A'CTAG_T Cuts at: 152 152 Size: 6559 SphI G_CATG'C Cuts at: 1132 3917 1132 Size: 2785 3774 SspI AAT'ATT Cuts at: 5 52 2318 3677 4483 5 Size: 47 2266 1359 806 2081 Fragments arranged by size: 2266 2081 1359 806 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6559 StyI C'CwwG_G Cuts at: 513 1065 2004 3054 3998 513 Size: 552 939 1050 944 3074 Fragments arranged by size: 3074 1050 944 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 4586 4603 4756 4941 5386 325 Size: 1255 567 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 567 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3189 4805 Size: 274 80 33 51 1443 618 594 1616 Cuts at: 4805 6517 96 Size: 1712 138 Fragments arranged by size: 1712 1616 1443 618 594 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3835 3867 6519 6551 3835 Size: 32 2652 32 3843 Fragments arranged by size: 3843 2652 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6559 XbaI T'CTAG_A Cuts at: 3586 3734 3895 3586 Size: 148 161 6250 Fragments arranged by size: 6250 161 148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2650 3069 2650 Size: 419 6140 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6559 XmaI C'CCGG_G Cuts at: 1870 3663 3884 1870 Size: 1793 221 4545 Fragments arranged by size: 4545 1793 221 XmnI GAAnn'nnTTC Cuts at: 2301 2753 4688 2301 Size: 452 1935 4172 Fragments arranged by size: 4172 1935 452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4367 276 Size: 53 83 186 936 2833 2468 Fragments arranged by size: 2833 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI