(Circular) (Six-base) MAPSORT of: pFN21AB7288.seq Check: 8961 from: 1 to: 8338 pFN21AB7288 8338 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2376 3809 4263 4801 2376 Size: 1433 454 538 5913 Fragments arranged by size: 5913 1433 538 454 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6148 278 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4272 4533 4569 5681 1937 Size: 2335 261 36 1112 4594 Fragments arranged by size: 4594 2335 1112 261 36 Acc65I G'GTAC_C Cuts at: 5659 5659 Size: 8338 AclI AA'CG_TT Cuts at: 1563 6465 6838 1563 Size: 4902 373 3063 Fragments arranged by size: 4902 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4045 4604 6401 7785 8053 1632 Size: 2413 559 1797 1384 268 1917 Fragments arranged by size: 2413 1917 1797 1384 559 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8141 AflIII A'CryG_T Cuts at: 1203 5355 7237 8214 1203 Size: 4152 1882 977 1327 Fragments arranged by size: 4152 1882 1327 977 AgeI A'CCGG_T Cuts at: 7169 7169 Size: 8338 AhdI GACnn_n'nnGTC Cuts at: 7067 7067 Size: 8338 AleI CACnn'nnGTG Cuts at: 1166 2444 3743 5306 1166 Size: 1278 1299 1563 4198 Fragments arranged by size: 4198 1563 1299 1278 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3156 3969 4829 1184 Size: 1972 813 860 4693 Fragments arranged by size: 4693 1972 860 813 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2552 Size: 32 8306 AlwNI CAG_nnn'CTG Cuts at: 2526 2683 3019 3370 4068 4167 5137 6081 7653 Size: 157 336 351 698 99 970 944 1572 Cuts at: 7653 2526 Size: 3211 Fragments arranged by size: 3211 1572 970 944 698 351 336 157 99 ApaI G_GGCC'C Cuts at: 2151 3600 4436 4582 4953 2151 Size: 1449 836 146 371 5536 Fragments arranged by size: 5536 1449 836 371 146 ApaLI G'TGCA_C Cuts at: 3511 3621 4865 6395 7551 3511 Size: 110 1244 1530 1156 4298 Fragments arranged by size: 4298 1530 1244 1156 110 ApoI r'AATT_y Cuts at: 1493 5647 5873 6025 8134 1493 Size: 4154 226 152 2109 1697 Fragments arranged by size: 4154 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6892 160 Size: 872 5860 1606 Fragments arranged by size: 5860 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 AvaI C'yCGr_G Cuts at: 1870 1943 2287 2402 2459 2666 2761 3448 3805 Size: 73 344 115 57 207 95 687 357 Cuts at: 3805 4482 4895 5663 1870 Size: 677 413 768 4545 Fragments arranged by size: 4545 768 687 677 413 357 344 207 115 95 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2855 2888 2855 Size: 33 8305 BamHI G'GATC_C Cuts at: 2008 5668 6035 2008 Size: 3660 367 4311 Fragments arranged by size: 4311 3660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2182 2398 2435 2927 3310 Size: 325 217 627 395 216 37 492 383 Cuts at: 3310 4445 4985 5659 7114 618 Size: 1135 540 674 1455 1842 Fragments arranged by size: 1842 1455 1135 674 627 540 492 395 383 325 217 216 37 BanII G_rGCy'C Cuts at: 1391 2151 2263 2454 3600 4436 4582 4926 4953 Size: 760 112 191 1146 836 146 344 27 Cuts at: 4953 5561 5598 5657 1391 Size: 608 37 59 4072 Fragments arranged by size: 4072 1146 836 760 608 344 191 146 112 59 37 27 BbeI G_GCGC'C Cuts at: 2402 3314 2402 Size: 912 7426 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4137 928 Size: 971 2238 5129 Fragments arranged by size: 5129 2238 971 BbvCI CC'TCA_GC Cuts at: 2720 3208 2720 Size: 488 7850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6529 6563 1903 Size: 34 4592 34 3678 Fragments arranged by size: 4592 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6230 7440 6230 Size: 1210 7128 BclI T'GATC_A Cuts at: 1548 1548 Size: 8338 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2155 2267 6949 Size: 107 122 71 631 605 483 112 4682 Cuts at: 6949 136 Size: 1525 Fragments arranged by size: 4682 1525 631 605 483 122 112 107 71 BglII A'GATC_T Cuts at: 5172 8333 5172 Size: 3161 5177 BlpI GC'TnA_GC Cuts at: 2519 2613 3371 5755 2519 Size: 94 758 2384 5102 Fragments arranged by size: 5102 2384 758 94 Bme1580I G_kGCm'C Cuts at: 1790 2151 2160 2187 2438 2776 2932 3515 3600 Size: 361 9 27 251 338 156 583 85 Cuts at: 3600 3625 4187 4436 4582 4869 4953 5382 6399 Size: 25 562 249 146 287 84 429 1017 Cuts at: 6399 7555 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1017 583 562 429 361 338 287 251 249 156 146 85 84 27 25 9 BmgBI CAC'GTC Cuts at: 1318 2485 2655 4345 5356 1318 Size: 1167 170 1690 1011 4300 Fragments arranged by size: 4300 1690 1167 1011 170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4621 4954 5369 Size: 416 53 83 399 24 3204 333 415 Cuts at: 5369 6069 7027 442 Size: 700 958 1753 Fragments arranged by size: 3204 1753 958 700 416 415 399 333 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3032 3041 4102 4328 5428 5444 6998 1239 Size: 1793 9 1061 226 1100 16 1554 2579 Fragments arranged by size: 2579 1793 1554 1100 1061 226 16 9 Bpu10I CC'TnA_GC Cuts at: 2720 2966 3208 2720 Size: 246 242 7850 Fragments arranged by size: 7850 246 242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6463 7328 7626 6463 Size: 865 298 7175 Fragments arranged by size: 7175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 3986 4492 4599 7001 882 Size: 617 853 1634 506 107 2402 2219 Fragments arranged by size: 2402 2219 1634 853 617 506 107 BsaAI yAC'GTr Cuts at: 493 1204 2224 2863 3241 3728 5125 6129 493 Size: 711 1020 639 378 487 1397 1004 2702 Fragments arranged by size: 2702 1397 1020 1004 711 639 487 378 BsaBI GATnn'nnATC Cuts at: 8332 8332 Size: 8338 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2199 2399 2736 3311 Size: 53 83 186 936 666 200 337 575 Cuts at: 3311 3918 4056 4275 4887 6145 6527 7162 275 Size: 607 138 219 612 1258 382 635 1451 Fragments arranged by size: 1451 1258 936 666 635 612 607 575 382 337 219 200 186 138 83 53 BsaWI w'CCGG_w Cuts at: 2280 2550 3166 3522 5166 6771 7169 7443 7590 Size: 270 616 356 1644 1605 398 274 147 Cuts at: 7590 8001 2280 Size: 411 2617 Fragments arranged by size: 2617 1644 1605 616 411 398 356 274 270 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 2644 2674 3188 Size: 30 165 30 1149 30 62 30 514 Cuts at: 3188 3218 3371 3401 3992 4022 4376 4406 1178 Size: 30 153 30 591 30 354 30 5110 Fragments arranged by size: 5110 1149 591 514 354 165 153 62 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2324 2327 2497 2573 2576 2579 2652 2759 Size: 1138 3 170 76 3 3 73 107 Cuts at: 2759 3196 3890 5060 1186 Size: 437 694 1170 4464 Fragments arranged by size: 4464 1170 1138 694 437 170 107 76 73 3 3 3 BseYI C'CCAG_C Cuts at: 2818 3036 3244 3295 3441 3693 3921 4746 7541 Size: 218 208 51 146 252 228 825 2795 Cuts at: 7541 8037 2818 Size: 496 3119 Fragments arranged by size: 3119 2795 825 496 252 228 218 208 146 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2348 2861 3163 3341 3368 3440 3574 Size: 573 612 513 302 178 27 72 134 Cuts at: 3574 1163 Size: 5927 Fragments arranged by size: 5927 612 573 513 302 178 134 72 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4411 5555 5788 6549 7577 1540 Size: 275 186 2410 1144 233 761 1028 2301 Fragments arranged by size: 2410 2301 1144 1028 761 275 233 186 BsiHKAI G_wGCw'C Cuts at: 2751 2862 3258 3515 3625 3912 3948 4604 4869 Size: 111 396 257 110 287 36 656 265 Cuts at: 4869 4926 4967 5007 5598 6399 6484 7555 2751 Size: 57 41 40 591 801 85 1071 3534 Fragments arranged by size: 3534 1071 801 656 591 396 287 265 257 111 110 85 57 41 40 36 BsmI GAATG_Cn' Cuts at: 4745 4830 5849 5942 4745 Size: 85 1019 93 7141 Fragments arranged by size: 7141 1019 93 85 BsmBI CGTCTCn'nnnn_ Cuts at: 2475 4392 4409 2475 Size: 1917 17 6404 Fragments arranged by size: 6404 1917 17 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2160 2187 2263 2438 2454 2751 Size: 399 361 9 27 76 175 16 297 Cuts at: 2751 2776 2862 2932 3258 3515 3600 3625 3912 Size: 25 86 70 326 257 85 25 287 Cuts at: 3912 3948 4187 4436 4582 4604 4869 4926 4953 Size: 36 239 249 146 22 265 57 27 Cuts at: 4953 4967 5007 5382 5561 5598 5657 6399 6484 Size: 14 40 375 179 37 59 742 85 Cuts at: 6484 7555 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 375 361 326 297 287 265 257 249 239 179 175 146 86 85 85 76 70 59 57 40 37 36 27 27 25 25 22 16 14 9 BspEI T'CCGG_A Cuts at: 2280 2550 3522 5166 8001 2280 Size: 270 972 1644 2835 2617 Fragments arranged by size: 2835 2617 1644 972 270 BspHI T'CATG_A Cuts at: 2886 6227 7957 2886 Size: 3341 1730 3267 Fragments arranged by size: 3341 3267 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2370 2376 2883 3390 3809 4263 4801 5078 Size: 1526 6 507 507 419 454 538 277 Cuts at: 5078 5693 844 Size: 615 3489 Fragments arranged by size: 3489 1526 615 538 507 507 454 419 277 6 BsrBI CCG'CTC Cuts at: 1394 1823 2027 2083 2165 4860 4996 6225 1394 Size: 429 204 56 82 2695 136 1229 3507 Fragments arranged by size: 3507 2695 1229 429 204 136 82 56 BsrDI GCAATG_nn' Cuts at: 65 1248 3482 4368 4888 6833 7007 65 Size: 1183 2234 886 520 1945 174 1396 Fragments arranged by size: 2234 1945 1396 1183 886 520 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2489 2643 3152 3430 6982 7169 Size: 110 204 475 154 509 278 3552 187 Cuts at: 7169 1700 Size: 2869 Fragments arranged by size: 3552 2869 509 475 278 204 187 154 110 BsrGI T'GTAC_A Cuts at: 96 5289 96 Size: 5193 3145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8338 BssSI C'ACGA_G Cuts at: 2393 3941 6398 7410 2393 Size: 1548 2457 1012 3321 Fragments arranged by size: 3321 2457 1548 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4725 794 Size: 3931 4407 BstBI TT'CG_AA Cuts at: 7142 7142 Size: 8338 BstEII G'GTnAC_C Cuts at: 1184 4197 1184 Size: 3013 5325 BstXI CCAn_nnnn'nTGG Cuts at: 2446 3233 4381 4793 2446 Size: 787 1148 412 5991 Fragments arranged by size: 5991 1148 787 412 BstYI r'GATC_y Cuts at: 1972 2008 4233 5104 5172 5668 6035 6420 6437 Size: 36 2225 871 68 496 367 385 17 Cuts at: 6437 7889 7975 7987 8333 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2225 1977 1452 871 496 385 367 346 86 68 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2848 4374 4428 4441 4678 5016 Size: 552 939 844 1526 54 13 237 338 Cuts at: 5016 5391 5506 513 Size: 375 115 3345 Fragments arranged by size: 3345 1526 939 844 552 375 338 237 115 54 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2544 487 Size: 683 56 1318 6281 Fragments arranged by size: 6281 1318 683 56 BtsI GCAGTG_nn' Cuts at: 3649 3887 4165 4876 5188 5854 6648 6668 3649 Size: 238 278 711 312 666 794 20 5319 Fragments arranged by size: 5319 794 711 666 312 278 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8338 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3259 3294 4594 4629 529 Size: 35 2695 35 1300 35 4238 Fragments arranged by size: 4238 2695 1300 35 35 35 DraI TTT'AAA Cuts at: 3643 5642 3643 Size: 1999 6339 DraIII CAC_nnn'GTG Cuts at: 2227 2227 Size: 8338 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4484 4544 4565 7345 809 Size: 507 3168 60 21 2780 1802 Fragments arranged by size: 3168 2780 1802 507 60 21 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2353 2487 2645 Size: 54 1425 50 275 18 523 134 158 Cuts at: 2645 3235 3428 3783 4293 4408 4997 5402 5785 Size: 590 193 355 510 115 589 405 383 Cuts at: 5785 6674 8303 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 590 589 523 510 405 383 355 275 193 158 134 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4408 5785 1537 Size: 275 2596 1377 4090 Fragments arranged by size: 4090 2596 1377 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 4329 4548 5111 6268 1353 Size: 983 1993 219 563 1157 3423 Fragments arranged by size: 3423 1993 1157 983 563 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2001 2782 4632 4813 6925 7299 Size: 1064 138 412 781 1850 181 2112 374 Cuts at: 7299 7445 387 Size: 146 1280 Fragments arranged by size: 2112 1850 1280 1064 781 412 374 181 146 138 EcoICRI GAG'CTC Cuts at: 4924 5596 4924 Size: 672 7666 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3032 3041 4045 4102 4328 4604 5428 Size: 393 1400 9 1004 57 226 276 824 Cuts at: 5428 5444 6401 6998 7785 8053 1239 Size: 16 957 597 787 268 1524 Fragments arranged by size: 1524 1400 1004 957 824 787 597 393 276 268 226 57 16 9 EcoNI CCTnn'n_nnAGG Cuts at: 3320 4553 3320 Size: 1233 7105 EcoO109I rG'GnC_Cy Cuts at: 2148 2680 2694 2812 3596 3597 3756 3895 4433 Size: 532 14 118 784 1 159 139 538 Cuts at: 4433 4579 4623 4950 5067 5428 2148 Size: 146 44 327 117 361 5058 Fragments arranged by size: 5058 784 538 532 361 327 159 146 139 118 117 44 14 1 EcoRI G'AATT_C Cuts at: 5647 6025 5647 Size: 378 7960 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4075 4342 4075 Size: 267 8071 FseI GG_CCGG'CC Cuts at: 2493 2493 Size: 8338 FspI TGC'GCA Cuts at: 2192 6844 2192 Size: 4652 3686 FspAI rTGC'GCAy Cuts at: 2192 2192 Size: 8338 HaeII r_GCGC'y Cuts at: 1678 2402 2519 3314 3437 3702 7485 1678 Size: 724 117 795 123 265 3783 2531 Fragments arranged by size: 3783 2531 795 724 265 123 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3188 3220 3639 3671 3992 Size: 32 135 32 1616 32 419 32 321 Cuts at: 3992 4024 4242 4274 5141 5173 6979 7011 7053 Size: 32 218 32 867 32 1806 32 42 Cuts at: 7053 7085 1373 Size: 32 2626 Fragments arranged by size: 2626 1806 1616 867 419 321 218 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4534 5682 5928 669 Size: 988 281 2596 1148 246 3079 Fragments arranged by size: 3079 2596 1148 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8338 HpaI GTT'AAC Cuts at: 5928 5928 Size: 8338 KasI G'GCGC_C Cuts at: 2398 3310 2398 Size: 912 7426 KpnI G_GTAC'C Cuts at: 5663 5663 Size: 8338 MfeI C'AATT_G Cuts at: 5937 5937 Size: 8338 MluI A'CGCG_T Cuts at: 8214 8214 Size: 8338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2458 7452 7636 1305 Size: 1153 4994 184 2007 Fragments arranged by size: 4994 2007 1153 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3237 10 Size: 54 1425 343 1405 5111 Fragments arranged by size: 5111 1425 1405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 2939 3155 3390 3743 3951 4223 Size: 648 1278 495 216 235 353 208 272 Cuts at: 4223 4373 4379 4469 4738 4811 4881 5306 5354 Size: 150 6 90 269 73 70 425 48 Cuts at: 5354 5396 6296 6655 6814 518 Size: 42 900 359 159 2042 Fragments arranged by size: 2042 1278 900 648 495 425 359 353 272 269 235 216 208 159 150 90 73 70 48 42 6 MspA1I CmG'CkG Cuts at: 1606 3036 3177 3276 3354 3547 3693 5018 5754 Size: 1430 141 99 78 193 146 1325 736 Cuts at: 5754 6431 7579 7824 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1430 1325 1148 736 677 245 193 146 141 99 78 NaeI GCC'GGC Cuts at: 2016 2491 2645 3432 2016 Size: 475 154 787 6922 Fragments arranged by size: 6922 787 475 154 NarI GG'CG_CC Cuts at: 2399 3311 2399 Size: 912 7426 NcoI C'CATG_G Cuts at: 513 1065 2004 4374 4428 5391 5506 513 Size: 552 939 2370 54 963 115 3345 Fragments arranged by size: 3345 2370 963 939 552 115 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 8338 NgoMIV G'CCGG_C Cuts at: 2014 2489 2643 3430 2014 Size: 475 154 787 6922 Fragments arranged by size: 6922 787 475 154 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8338 NotI GC'GGCC_GC Cuts at: 5785 5785 Size: 8338 NspI r_CATG'y Cuts at: 1132 1229 2633 2786 3133 3160 4810 5696 7241 Size: 97 1404 153 347 27 1650 886 1545 Cuts at: 7241 1132 Size: 2229 Fragments arranged by size: 2229 1650 1545 1404 886 347 153 97 27 PasI CC'CwG_GG Cuts at: 1348 2152 2203 2731 1348 Size: 804 51 528 6955 Fragments arranged by size: 6955 804 528 51 PciI A'CATG_T Cuts at: 7237 7237 Size: 8338 PflMI CCAn_nnn'nTGG Cuts at: 1108 3244 4291 4669 5333 5512 1108 Size: 2136 1047 378 664 179 3934 Fragments arranged by size: 3934 2136 1047 664 378 179 PfoI T'CCnGG_A Cuts at: 4548 4548 Size: 8338 PmeI GTTT'AAAC Cuts at: 5642 5642 Size: 8338 PmlI CAC'GTG Cuts at: 2224 3241 2224 Size: 1017 7321 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2584 6407 6439 7920 7952 2552 Size: 32 3823 32 1481 32 2938 Fragments arranged by size: 3823 2938 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2812 3756 3895 4623 5067 5428 2812 Size: 944 139 728 444 361 5722 Fragments arranged by size: 5722 944 728 444 361 139 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8338 PsiI TTA'TAA Cuts at: 5908 5908 Size: 8338 PspOMI G'GGCC_C Cuts at: 2147 3596 4432 4578 4949 2147 Size: 1449 836 146 371 5536 Fragments arranged by size: 5536 1449 836 371 146 PspXI vC'TCGA_Gb Cuts at: 2761 2761 Size: 8338 PstI C_TGCA'G Cuts at: 830 3359 3836 4088 4243 4425 4916 5075 5690 Size: 2529 477 252 155 182 491 159 615 Cuts at: 5690 830 Size: 3478 Fragments arranged by size: 3478 2529 615 491 477 252 182 159 155 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8338 PvuII CAG'CTG Cuts at: 3177 3547 3693 3177 Size: 370 146 7822 Fragments arranged by size: 7822 370 146 RsrII CG'GwC_CG Cuts at: 2278 3169 2278 Size: 891 7447 SacI G_AGCT'C Cuts at: 4926 5598 4926 Size: 672 7666 SacII CC_GC'GG Cuts at: 5019 5019 Size: 8338 SalI G'TCGA_C Cuts at: 1936 4532 5680 1936 Size: 2596 1148 4594 Fragments arranged by size: 4594 2596 1148 SanDI GG'GwC_CC Cuts at: 4623 4623 Size: 8338 SapI GCTCTTCn'nnn_ Cuts at: 5111 5111 Size: 8338 SbfI CC_TGCA'GG Cuts at: 4243 5075 5690 4243 Size: 832 615 6891 Fragments arranged by size: 6891 832 615 ScaI AGT'ACT Cuts at: 6586 8298 6586 Size: 1712 6626 SfcI C'TryA_G Cuts at: 826 1045 3355 3832 4084 4239 4421 4912 5071 Size: 219 2310 477 252 155 182 491 159 Cuts at: 5071 5215 5385 5686 6821 7502 7693 826 Size: 144 170 301 1135 681 191 1471 Fragments arranged by size: 2310 1471 1135 681 491 477 301 252 219 191 182 170 159 155 144 SfoI GGC'GCC Cuts at: 2400 3312 2400 Size: 912 7426 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8338 SgrAI Cr'CCGG_yG Cuts at: 1700 2014 1700 Size: 314 8024 SmaI CCC'GGG Cuts at: 1872 2404 3450 4484 5665 1872 Size: 532 1046 1034 1181 4545 Fragments arranged by size: 4545 1181 1046 1034 532 SmlI C'TyrA_G Cuts at: 820 1017 1943 2761 6442 7343 7605 820 Size: 197 926 818 3681 901 262 1553 Fragments arranged by size: 3681 1553 926 901 818 262 197 SnaBI TAC'GTA Cuts at: 493 5125 6129 493 Size: 4632 1004 2702 Fragments arranged by size: 4632 2702 1004 SpeI A'CTAG_T Cuts at: 152 152 Size: 8338 SphI G_CATG'C Cuts at: 1132 2786 3133 4810 5696 1132 Size: 1654 347 1677 886 3774 Fragments arranged by size: 3774 1677 1654 886 347 SspI AAT'ATT Cuts at: 5 52 5211 6262 5 Size: 47 5159 1051 2081 Fragments arranged by size: 5159 2081 1051 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8338 StyI C'CwwG_G Cuts at: 513 1065 2004 2583 2922 3603 3957 4374 4428 Size: 552 939 579 339 681 354 417 54 Cuts at: 4428 5194 5391 5506 5777 513 Size: 766 197 115 271 3074 Fragments arranged by size: 3074 939 766 681 579 552 417 354 339 271 197 115 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2665 2915 4216 5003 5484 5548 5569 Size: 1255 1085 250 1301 787 481 64 21 Cuts at: 5569 6365 6382 6535 6720 7165 325 Size: 796 17 153 185 445 1498 Fragments arranged by size: 1498 1301 1255 1085 796 787 481 445 250 185 153 64 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3900 3975 4392 Size: 274 80 33 51 1443 1923 75 417 Cuts at: 4392 5289 6584 8296 96 Size: 897 1295 1712 138 Fragments arranged by size: 1923 1712 1443 1295 897 417 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2644 2676 3604 3636 3657 3689 4121 4153 8298 Size: 32 928 32 21 32 432 32 4145 Cuts at: 8298 8330 2644 Size: 32 2652 Fragments arranged by size: 4145 2652 928 432 32 32 32 32 32 21 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8338 XbaI T'CTAG_A Cuts at: 5674 5674 Size: 8338 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4018 4288 4456 5389 4018 Size: 270 168 933 6967 Fragments arranged by size: 6967 933 270 168 XhoI C'TCGA_G Cuts at: 1943 2761 1943 Size: 818 7520 XmaI C'CCGG_G Cuts at: 1870 2402 3448 4482 5663 1870 Size: 532 1046 1034 1181 4545 Fragments arranged by size: 4545 1181 1046 1034 532 XmnI GAAnn'nnTTC Cuts at: 6467 6467 Size: 8338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6146 276 Size: 53 83 186 936 4612 2468 Fragments arranged by size: 4612 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV NruI NsiI PacI PsrI SexAI SfiI SrfI SwaI