Restriction map for
pFN21AA1996
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI Alw44I
AlwI AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 1170 AACGTT AA^CGTT
AfaI 712 GTAC GT^AC
AhdI 958 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 944 AGCGCT AGC^GCT
Asp700I 700 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 958 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 968 CCCGAG C^[CT]CG[GA]G
BanII 951 GGGCTC G[GA]GC[CT]^C
BbvCI 1111 CCTCAGC CCTCAGC(-5/-2)
BclI 513 TGATCA T^GATCA
BcnI 822 CCGGG CC^[GC]GG
BglII 162 AGATCT A^GATCT
BmyI 951 GGGCTC G[AGT]GC[ACT]^C
BsaI 1128 GAGACC GGTCTC(1/5)
BsmFI 966 GTCCC GGGAC(10/14)
BsmI 491 GCATTC GAATGC(1/-1)
BsoBI 968 CCCGAG C^[CT]CG[GA]G
Bsp1286I 951 GGGCTC G[AGT]GC[ACT]^C
BsrDI 1097 CATTGC GCAATG(2/0)
BsrFI 914 GCCGGC [GA]^CCGG[CT]
BssSI 997 CTCGTG CACGAG(-5/-1)
BstF5I 884 GGATG GGATG(2/0)
BstXI 1132 CCATCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 162 AGATCT [GA]^GATC[CT]
Cfr10I 914 GCCGGC [GA]^CCGG[CT]
DraII 964 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 677 CCATGG C^C[GA][CT]GG
Eam1105I 958 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 944 AGCGCT AGC^GCT
EcoO109I 964 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 513 TGATCA T^GATCA
FokI 884 GGATG GGATG(9/13)
HincII 671 GTCAAC GT[CT]^[GA]AC
HindII 671 GTCAAC GT[CT]^[GA]AC
MflI 162 AGATCT [GA]^GATC[CT]
MseI 206 TTAA T^TAA
MslI 1133 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 807 CAGCGG C[AC]G^C[GT]G
NaeI 914 GCCGGC GCC^GGC
NciI 822 CCGGG CC^[GC]GG
NcoI 677 CCATGG C^CATGG
NgoMIV 914 GCCGGC G^CCGGC
NspI 1022 ACATGC [GA]CATG^[CT]
PflMI 1178 CCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 964 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1170 AACGTT AA^CGTT
RsaI 712 GTAC GT^AC
SfaNI 885 GATGC GCATC(5/9)
SmlI 894 CTTGAG C^T[CT][GA]AG
Tru9I 206 TTAA T^TAA
Van91I 1178 CCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 668 CCAGTCAACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 162 AGATCT [GA]^GATC[CT]
XmnI 700 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 809 GCGG CCGC(-3/-1)
836 GCGG
AluI 216 AGCT AG^CT
619 AGCT
AlwNI 1039 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1048 CAGAAACTG
BbvI 974 GCAGC GCAGC(8/12)
90 GCTGC
BseRI 1109 CTCCTC GAGGAG(10/8)
785 GAGGAG
BspMI 744 GCAGGT ACCTGC(4/8)
918 GCAGGT
BstNI 718 CCTGG CC^[AT]GG
1159 CCTGG
Cac8I 914 GCCGGC GC[ACGT]^[ACGT]GC
1074 GCAGGC
CfoI 814 GCGC GCG^C
945 GCGC
EcoRI 536 GAATTC G^AATTC
850 GAATTC
EcoRII 718 CCTGG ^CC[AT]GG
1159 CCTGG
EcoT14I 677 CCATGG C^C[AT][AT]GG
908 CCAAGG
Fnu4HI 90 GCTGC GC^[ACGT]GC
974 GCAGC
HaeII 813 AGCGCC [GA]GCGC^[CT]
944 AGCGCT
HapII 822 CCGG C^CGG
915 CCGG
HhaI 814 GCGC GCG^C
945 GCGC
HinP1I 814 GCGC G^CGC
945 GCGC
HpaII 822 CCGG C^CGG
915 CCGG
ItaI 90 GCTGC GC^[ACGT]GC
974 GCAGC
MaeII 625 ACGT A^CGT
1171 ACGT
MspI 822 CCGG C^CGG
915 CCGG
MvaI 718 CCTGG CC^[AT]GG
1159 CCTGG
MwoI 16 GCCATGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1069 GCTTTGCAGGC
NdeI 26 CATATG CA^TATG
1182 CATATG
PleI 241 GAGTC GAGTC(4/5)
607 GAGTC
PstI 1012 CTGCAG CTGCA^G
1045 CTGCAG
SfcI 1012 CTGCAG C^T[GA][CT]AG
1045 CTGCAG
StyI 677 CCATGG C^C[AT][AT]GG
908 CCAAGG
TaqI 122 TCGA T^CGA
1148 TCGA
TfiI 556 GATTC G^A[AT]TC
580 GATTC
TseI 90 GCTGC G^C[AT]GC
974 GCAGC
Tsp45I 227 GTGAC ^GT[GC]AC
349 GTCAC
TthHB8I 122 TCGA T^CGA
1148 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI ApoI AvaII BsaJI BsiYI
BslI BsmAI BsrI BssKI Cfr13I
DdeI DpnII EarI HaeIII HinfI
HphI Ksp632I MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tsp509I TspRI