(Circular) (Six-base) MAPSORT of: pFN21AA1996.seq Check: 851 from: 1 to: 5953 pFN21AA1996 5953 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2740 2914 2740 Size: 174 5779 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 3176 3252 4080 4453 1563 Size: 1613 76 828 373 3063 Fragments arranged by size: 3063 1613 828 373 76 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2460 3026 4016 5400 5668 1632 Size: 828 566 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 828 566 268 AfeI AGC'GCT Cuts at: 2951 2951 Size: 5953 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 4852 5829 1203 Size: 3649 977 1327 Fragments arranged by size: 3649 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 2968 4682 2968 Size: 1714 4239 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2772 1184 Size: 1588 4365 AlwNI CAG_nnn'CTG Cuts at: 3049 3058 3696 5268 3049 Size: 9 638 1572 3734 Fragments arranged by size: 3734 1572 638 9 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 2338 2541 2689 2855 3262 3488 3640 5749 Size: 845 203 148 166 407 226 152 2109 Cuts at: 5749 1493 Size: 1697 Fragments arranged by size: 2109 1697 845 407 226 203 166 152 148 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 2973 3278 1870 Size: 73 1030 305 4545 Fragments arranged by size: 4545 1030 305 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3274 4729 618 Size: 325 217 627 1487 1455 1842 Fragments arranged by size: 1842 1487 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2960 3272 1391 Size: 1569 312 4072 Fragments arranged by size: 4072 1569 312 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4982 BbvCI CC'TCA_GC Cuts at: 3117 3117 Size: 5953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 2518 1548 Size: 970 4983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4564 136 Size: 107 122 71 631 605 2892 1525 Fragments arranged by size: 2892 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2167 5948 2167 Size: 3781 2172 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5953 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2999 2999 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4613 1239 Size: 3374 2579 Bpu10I CC'TnA_GC Cuts at: 3117 3117 Size: 5953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2920 4078 4943 5241 2920 Size: 1158 865 298 3632 Fragments arranged by size: 3632 1158 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3127 4616 882 Size: 617 1628 1489 2219 Fragments arranged by size: 2219 1628 1489 617 BsaAI yAC'GTr Cuts at: 493 1204 3744 493 Size: 711 2540 2702 Fragments arranged by size: 2702 2540 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3760 4142 4777 275 Size: 53 83 186 936 2227 382 635 1451 Fragments arranged by size: 2227 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2176 2206 1178 Size: 30 165 30 773 30 4925 Fragments arranged by size: 4925 773 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2805 3105 1186 Size: 1619 300 4034 Fragments arranged by size: 4034 1619 300 BseYI C'CCAG_C Cuts at: 2952 5156 5652 2952 Size: 2204 496 3253 Fragments arranged by size: 3253 2204 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3403 4164 5192 1540 Size: 275 186 1402 761 1028 2301 Fragments arranged by size: 2301 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4014 4099 5170 4014 Size: 85 1071 4797 Fragments arranged by size: 4797 1071 85 BsmI GAATG_Cn' Cuts at: 2496 3464 3557 2496 Size: 968 93 4892 Fragments arranged by size: 4892 968 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2960 3272 4014 4099 5170 1391 Size: 399 1170 312 742 85 1071 2174 Fragments arranged by size: 2174 1170 1071 742 399 312 85 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2740 2914 3308 844 Size: 1896 174 394 3489 Fragments arranged by size: 3489 1896 394 174 BsrBI CCG'CTC Cuts at: 1394 1823 3840 1394 Size: 429 2017 3507 Fragments arranged by size: 3507 2017 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3101 4448 4622 65 Size: 1183 1853 1347 174 1396 Fragments arranged by size: 1853 1396 1347 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2919 4597 4784 1700 Size: 110 1109 1678 187 2869 Fragments arranged by size: 2869 1678 1109 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 3002 4013 5025 3002 Size: 1011 1012 3930 Fragments arranged by size: 3930 1012 1011 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstXI CCAn_nnnn'nTGG Cuts at: 3144 3144 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2167 3283 3650 4035 4052 5504 5590 5602 Size: 195 1116 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3784 Fragments arranged by size: 3784 1617 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2236 3469 4263 4283 2236 Size: 1233 794 20 3906 Fragments arranged by size: 3906 1233 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2122 2157 529 Size: 35 1558 35 4325 Fragments arranged by size: 4325 1558 35 35 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918 Size: 54 1425 50 275 18 1570 889 1629 Cuts at: 5918 8 Size: 43 Fragments arranged by size: 1629 1570 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2604 2866 2872 3883 1353 Size: 900 351 262 6 1011 3423 Fragments arranged by size: 3423 1011 900 351 262 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2856 4540 4914 5060 387 Size: 1064 138 1267 1684 374 146 1280 Fragments arranged by size: 1684 1280 1267 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2460 3026 4016 4613 5400 5668 1239 Size: 393 828 566 990 597 787 268 1524 Fragments arranged by size: 1524 990 828 787 597 566 393 268 EcoO109I rG'GnC_Cy Cuts at: 2970 2970 Size: 5953 EcoRI G'AATT_C Cuts at: 2541 2855 3262 3640 2541 Size: 314 407 378 4854 Fragments arranged by size: 4854 407 378 314 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2822 2953 5100 1678 Size: 1144 131 2147 2531 Fragments arranged by size: 2531 2147 1144 131 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2843 2875 2954 Size: 32 135 32 575 32 664 32 79 Cuts at: 2954 2986 3100 3132 4594 4626 4668 4700 1373 Size: 32 114 32 1462 32 42 32 2626 Fragments arranged by size: 2626 1462 664 575 135 114 79 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2678 3297 3543 669 Size: 988 281 740 619 246 3079 Fragments arranged by size: 3079 988 740 619 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2658 2985 5067 5251 1305 Size: 1353 327 2082 184 2007 Fragments arranged by size: 2082 2007 1353 327 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4131 Fragments arranged by size: 4131 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3142 3911 4270 4429 518 Size: 648 1976 769 359 159 2042 Fragments arranged by size: 2042 1976 769 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2814 3369 4046 5194 5439 1606 Size: 1208 555 677 1148 245 2120 Fragments arranged by size: 2120 1208 1148 677 555 245 NaeI GCC'GGC Cuts at: 2921 2921 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3784 Fragments arranged by size: 3784 1617 552 NdeI CA'TA_TG Cuts at: 387 2032 3188 387 Size: 1645 1156 3152 Fragments arranged by size: 3152 1645 1156 NgoMIV G'CCGG_C Cuts at: 2919 2919 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 3031 3311 4856 1132 Size: 97 1802 280 1545 2229 Fragments arranged by size: 2229 1802 1545 280 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 3189 1108 Size: 2081 3872 PfoI T'CCnGG_A Cuts at: 2722 2722 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2970 2970 Size: 5953 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PstI C_TGCA'G Cuts at: 830 3021 3054 3305 830 Size: 2191 33 251 3478 Fragments arranged by size: 3478 2191 251 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SanDI GG'GwC_CC Cuts at: 2970 2970 Size: 5953 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SfcI C'TryA_G Cuts at: 826 1045 3017 3050 3301 4436 5117 5308 826 Size: 219 1972 33 251 1135 681 191 1471 Fragments arranged by size: 1972 1471 1135 681 251 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2899 4057 4958 5220 820 Size: 197 926 956 1158 901 262 1553 Fragments arranged by size: 1553 1158 956 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2682 2913 3392 513 Size: 552 1617 231 479 3074 Fragments arranged by size: 3074 1617 552 479 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 3980 3997 4150 4335 4780 325 Size: 1255 1071 1329 17 153 185 445 1498 Fragments arranged by size: 1498 1329 1255 1071 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4199 5911 96 Size: 274 80 33 51 1443 2222 1712 138 Fragments arranged by size: 2222 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 5913 5945 2176 Size: 32 3705 32 2184 Fragments arranged by size: 3705 2184 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2680 2680 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 2709 4082 2709 Size: 1373 4580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI