Restriction map for
pFN21AA1996
(Linear) (Six-base) MAPSORT of: KIAA1996.seq Check: 5971 from: 1 to: 1248
KIAA1996.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 735 909 1248
Size: 735 174 339
Fragments arranged by size:
735 339 174
AclI AA'CG_TT
Cuts at: 0 1171 1248
Size: 1171 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1021 1248
Size: 455 566 227
Fragments arranged by size:
566 455 227
AfeI AGC'GCT
Cuts at: 0 946 1248
Size: 946 302
AhdI GACnn_n'nnGTC
Cuts at: 0 963 1248
Size: 963 285
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 1248
Size: 767 481
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1053 1248
Size: 1044 9 195
Fragments arranged by size:
1044 195 9
ApoI r'AATT_y
Cuts at: 0 333 536 684 850 1248
Size: 333 203 148 166 398
Fragments arranged by size:
398 333 203 166 148
AvaI C'yCGr_G
Cuts at: 0 968 1248
Size: 968 280
BanII G_rGCy'C
Cuts at: 0 955 1248
Size: 955 293
BbvCI CC'TCA_GC
Cuts at: 0 1112 1248
Size: 1112 136
BclI T'GATC_A
Cuts at: 0 513 1248
Size: 513 735
BglII A'GATC_T
Cuts at: 0 162 1248
Size: 162 1086
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 994 1248
Size: 994 254
Bpu10I CC'TnA_GC
Cuts at: 0 1112 1248
Size: 1112 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1248
Size: 915 333
BsaI GGTCTCn'nnnn_
Cuts at: 0 1122 1248
Size: 1122 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 171 201 1248
Size: 171 30 1047
Fragments arranged by size:
1047 171 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 800 1100 1248
Size: 800 300 148
Fragments arranged by size:
800 300 148
BseYI C'CCAG_C
Cuts at: 0 947 1248
Size: 947 301
BsmI GAATG_Cn'
Cuts at: 0 491 1248
Size: 491 757
Bsp1286I G_dGCh'C
Cuts at: 0 955 1248
Size: 955 293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 735 909 1248
Size: 735 174 339
Fragments arranged by size:
735 339 174
BsrDI GCAATG_nn'
Cuts at: 0 1096 1248
Size: 1096 152
BsrFI r'CCGG_y
Cuts at: 0 914 1248
Size: 914 334
BssSI C'ACGA_G
Cuts at: 0 997 1248
Size: 997 251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1139 1248
Size: 1139 109
BstYI r'GATC_y
Cuts at: 0 162 1248
Size: 162 1086
BtgI C'CryG_G
Cuts at: 0 677 1248
Size: 677 571
BtsI GCAGTG_nn'
Cuts at: 0 231 1248
Size: 231 1017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 117 152 1248
Size: 117 35 1096
Fragments arranged by size:
1096 117 35
EarI CTCTTCn'nnn_
Cuts at: 0 248 599 861 867 1248
Size: 248 351 262 6 381
Fragments arranged by size:
381 351 262 248 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 851 1248
Size: 851 397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1021 1248
Size: 455 566 227
Fragments arranged by size:
566 455 227
EcoO109I rG'GnC_Cy
Cuts at: 0 965 1248
Size: 965 283
EcoRI G'AATT_C
Cuts at: 0 536 850 1248
Size: 536 314 398
Fragments arranged by size:
536 398 314
HaeII r_GCGC'y
Cuts at: 0 817 948 1248
Size: 817 131 300
Fragments arranged by size:
817 300 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 838 870 949 981 1095 1127
Size: 142 32 664 32 79 32 114 32
Cuts at: 1127 1248
Size: 121
Fragments arranged by size:
664 142 121 114 79 32 32 32
32
HincII GTy'rAC
Cuts at: 0 673 1248
Size: 673 575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 980 1248
Size: 653 327 268
Fragments arranged by size:
653 327 268
MslI CAynn'nnrTG
Cuts at: 0 1137 1248
Size: 1137 111
MspA1I CmG'CkG
Cuts at: 0 809 1248
Size: 809 439
NaeI GCC'GGC
Cuts at: 0 916 1248
Size: 916 332
NcoI C'CATG_G
Cuts at: 0 677 1248
Size: 677 571
NdeI CA'TA_TG
Cuts at: 0 27 1183 1248
Size: 27 1156 65
Fragments arranged by size:
1156 65 27
NgoMIV G'CCGG_C
Cuts at: 0 914 1248
Size: 914 334
NspI r_CATG'y
Cuts at: 0 1026 1248
Size: 1026 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 1184 1248
Size: 1184 64
PfoI T'CCnGG_A
Cuts at: 0 717 1248
Size: 717 531
PpuMI rG'GwC_Cy
Cuts at: 0 965 1248
Size: 965 283
PstI C_TGCA'G
Cuts at: 0 1016 1049 1248
Size: 1016 33 199
Fragments arranged by size:
1016 199 33
SanDI GG'GwC_CC
Cuts at: 0 965 1248
Size: 965 283
SfcI C'TryA_G
Cuts at: 0 1012 1045 1248
Size: 1012 33 203
Fragments arranged by size:
1012 203 33
SmlI C'TyrA_G
Cuts at: 0 894 1248
Size: 894 354
StyI C'CwwG_G
Cuts at: 0 677 908 1248
Size: 677 231 340
Fragments arranged by size:
677 340 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 1248
Size: 646 602
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 171 203 1248
Size: 171 32 1045
Fragments arranged by size:
1045 171 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 1248
Size: 675 573
XmnI GAAnn'nnTTC
Cuts at: 0 704 1248
Size: 704 544
Enzymes that do cut:
AarI AclI AcuI AfeI AhdI AlfI AlwNI ApoI
AvaI BanII BbvCI BclI BglII BplI Bpu10I BpuEI
BsaI BsaXI BseRI BseYI BsmI Bsp1286I BspMI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtsI CspCI EarI
EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PflMI
PfoI PpuMI PstI SanDI SfcI SmlI StyI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI BpmI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI