(Circular) (Six-base) MAPSORT of: pFN21AA0822.seq Check: 1512 from: 1 to: 9448 pFN21AA0822 9448 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5404 5404 Size: 9448 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 7258 278 Size: 53 83 186 936 1762 3960 2468 Fragments arranged by size: 3960 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2238 4526 5737 6218 6638 6791 1937 Size: 301 2288 1211 481 420 153 4594 Fragments arranged by size: 4594 2288 1211 481 420 301 153 Acc65I G'GTAC_C Cuts at: 6769 6769 Size: 9448 AclI AA'CG_TT Cuts at: 1563 7575 7948 1563 Size: 6012 373 3063 Fragments arranged by size: 6012 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2388 2896 3335 3877 4268 5478 6137 7511 Size: 756 508 439 542 391 1210 659 1374 Cuts at: 7511 8895 9163 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1374 1210 756 659 542 508 439 391 268 AflII C'TTAA_G Cuts at: 820 1017 4018 820 Size: 197 3001 6250 Fragments arranged by size: 6250 3001 197 AflIII A'CryG_T Cuts at: 1203 8347 9324 1203 Size: 7144 977 1327 Fragments arranged by size: 7144 1327 977 AgeI A'CCGG_T Cuts at: 8279 8279 Size: 9448 AhdI GACnn_n'nnGTC Cuts at: 8177 8177 Size: 9448 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9448 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9448 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 2780 Size: 32 9416 AlwNI CAG_nnn'CTG Cuts at: 2618 5095 6022 6326 6682 7191 8763 2618 Size: 2477 927 304 356 509 1572 3303 Fragments arranged by size: 3303 2477 1572 927 509 356 304 ApaLI G'TGCA_C Cuts at: 7505 8661 7505 Size: 1156 8292 ApoI r'AATT_y Cuts at: 1493 2110 2348 3154 3208 3267 3422 4154 5592 Size: 617 238 806 54 59 155 732 1438 Cuts at: 5592 5692 6407 6757 6983 7135 9244 1493 Size: 100 715 350 226 152 2109 1697 Fragments arranged by size: 2109 1697 1438 806 732 715 617 350 238 226 155 152 100 59 54 AseI AT'TA_AT Cuts at: 160 1032 2556 4272 4487 8002 160 Size: 872 1524 1716 215 3515 1606 Fragments arranged by size: 3515 1716 1606 1524 872 215 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9448 AvaI C'yCGr_G Cuts at: 1870 1943 6233 6773 1870 Size: 73 4290 540 4545 Fragments arranged by size: 4545 4290 540 73 AvrII C'CTAG_G Cuts at: 4195 4195 Size: 9448 BamHI G'GATC_C Cuts at: 3826 5578 6778 7145 3826 Size: 1752 1200 367 6129 Fragments arranged by size: 6129 1752 1200 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6293 6769 8224 618 Size: 325 217 627 4506 476 1455 1842 Fragments arranged by size: 4506 1842 1455 627 476 325 217 BanII G_rGCy'C Cuts at: 1391 3977 5038 6465 6666 6767 1391 Size: 2586 1061 1427 201 101 4072 Fragments arranged by size: 4072 2586 1427 1061 201 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2774 2902 5116 5172 6137 928 Size: 971 875 128 2214 56 965 4239 Fragments arranged by size: 4239 2214 971 965 875 128 56 BbvCI CC'TCA_GC Cuts at: 6050 6066 6327 6050 Size: 16 261 9171 Fragments arranged by size: 9171 261 16 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7639 7673 1903 Size: 34 5702 34 3678 Fragments arranged by size: 5702 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7340 8550 7340 Size: 1210 8238 BclI T'GATC_A Cuts at: 1548 3349 4632 1548 Size: 1801 1283 6364 Fragments arranged by size: 6364 1801 1283 BfrBI ATG'CAT Cuts at: 2638 2723 3164 4712 2638 Size: 85 441 1548 7374 Fragments arranged by size: 7374 1548 441 85 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6266 8059 136 Size: 107 122 71 631 605 4594 1793 1525 Fragments arranged by size: 4594 1793 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2870 3505 9443 2870 Size: 635 5938 2875 Fragments arranged by size: 5938 2875 635 BlpI GC'TnA_GC Cuts at: 6865 6865 Size: 9448 Bme1580I G_kGCm'C Cuts at: 1790 6298 7509 8665 1790 Size: 4508 1211 1156 2573 Fragments arranged by size: 4508 2573 1211 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2872 3895 4826 Size: 416 53 83 399 24 1455 1023 931 Cuts at: 4826 6191 7179 8137 442 Size: 1365 988 958 1753 Fragments arranged by size: 1753 1455 1365 1023 988 958 931 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9448 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3755 Size: 9448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3402 5916 5978 6044 6449 6665 6680 8108 Size: 2163 2514 62 66 405 216 15 1428 Cuts at: 8108 1239 Size: 2579 Fragments arranged by size: 2579 2514 2163 1428 405 216 66 62 15 Bpu10I CC'TnA_GC Cuts at: 6050 6066 6173 6327 6050 Size: 16 107 154 9171 Fragments arranged by size: 9171 154 107 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6083 7573 8438 8736 6083 Size: 1490 865 298 6795 Fragments arranged by size: 6795 1490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3336 8111 882 Size: 617 1837 4775 2219 Fragments arranged by size: 4775 2219 1837 617 BsaAI yAC'GTr Cuts at: 493 1204 3355 5248 7239 493 Size: 711 2151 1893 1991 2702 Fragments arranged by size: 2702 2151 1991 1893 711 BsaBI GATnn'nnATC Cuts at: 3381 4185 6705 9442 3381 Size: 804 2520 2737 3387 Fragments arranged by size: 3387 2737 2520 804 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 7255 7637 8272 Size: 53 83 186 936 1762 3960 382 635 Cuts at: 8272 275 Size: 1451 Fragments arranged by size: 3960 1762 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3683 5057 6124 7881 8279 8553 8700 9111 3683 Size: 1374 1067 1757 398 274 147 411 4020 Fragments arranged by size: 4020 1757 1374 1067 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2594 2624 1178 Size: 30 165 30 1191 30 8002 Fragments arranged by size: 8002 1191 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 3002 4563 6511 6632 6710 6719 1186 Size: 1429 387 1561 1948 121 78 9 3915 Fragments arranged by size: 3915 1948 1561 1429 387 121 78 9 BseYI C'CCAG_C Cuts at: 3126 5043 6682 8651 9147 3126 Size: 1917 1639 1969 496 3427 Fragments arranged by size: 3427 1969 1917 1639 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8875 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6222 6898 7659 8687 1540 Size: 275 186 4221 676 761 1028 2301 Fragments arranged by size: 4221 2301 1028 761 676 275 186 BsiHKAI G_wGCw'C Cuts at: 2457 4686 5038 6666 7509 7594 8665 2457 Size: 2229 352 1628 843 85 1071 3240 Fragments arranged by size: 3240 2229 1628 1071 843 352 85 BsmI GAATG_Cn' Cuts at: 2442 2638 6959 7052 2442 Size: 196 4321 93 4838 Fragments arranged by size: 4838 4321 196 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2457 3977 4686 5038 6298 6465 6666 Size: 399 667 1520 709 352 1260 167 201 Cuts at: 6666 6767 7509 7594 8665 1391 Size: 101 742 85 1071 2174 Fragments arranged by size: 2174 1520 1260 1071 742 709 667 399 352 201 167 101 85 BspEI T'CCGG_A Cuts at: 5057 9111 5057 Size: 4054 5394 BspHI T'CATG_A Cuts at: 3394 5238 7337 9067 3394 Size: 1844 2099 1730 3775 Fragments arranged by size: 3775 2099 1844 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 5404 6432 6640 6803 844 Size: 1450 3110 1028 208 163 3489 Fragments arranged by size: 3489 3110 1450 1028 208 163 BsrBI CCG'CTC Cuts at: 1394 1823 5940 7335 1394 Size: 429 4117 1395 3507 Fragments arranged by size: 4117 3507 1395 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2114 4284 4813 4998 5724 7943 8117 Size: 1183 866 2170 529 185 726 2219 174 Cuts at: 8117 65 Size: 1396 Fragments arranged by size: 2219 2170 1396 1183 866 726 529 185 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2993 8092 8279 1700 Size: 110 1183 5099 187 2869 Fragments arranged by size: 5099 2869 1183 187 110 BsrGI T'GTAC_A Cuts at: 96 3051 5473 96 Size: 2955 2422 4071 Fragments arranged by size: 4071 2955 2422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9448 BssSI C'ACGA_G Cuts at: 5800 7508 8520 5800 Size: 1708 1012 6728 Fragments arranged by size: 6728 1708 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4808 794 Size: 4014 5434 BstBI TT'CG_AA Cuts at: 5163 5355 8252 5163 Size: 192 2897 6359 Fragments arranged by size: 6359 2897 192 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9448 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2194 Size: 9448 BstYI r'GATC_y Cuts at: 1972 2870 3505 3826 4174 5578 6477 6778 7145 Size: 898 635 321 348 1404 899 301 367 Cuts at: 7145 7530 7547 8999 9085 9097 9443 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1404 899 898 635 385 367 348 346 321 301 86 17 12 BstZ17I GTA'TAC Cuts at: 2239 4527 2239 Size: 2288 7160 Bsu36I CC'TnA_GG Cuts at: 5979 6482 5979 Size: 503 8945 BtgI C'CryG_G Cuts at: 513 1065 2187 4530 513 Size: 552 1122 2343 5431 Fragments arranged by size: 5431 2343 1122 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8709 Fragments arranged by size: 8709 683 56 BtsI GCAGTG_nn' Cuts at: 2177 3537 3575 4478 6964 7758 7778 2177 Size: 1360 38 903 2486 794 20 3847 Fragments arranged by size: 3847 2486 1360 903 794 38 20 ClaI AT'CG_AT Cuts at: 1554 5016 1554 Size: 3462 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5389 5424 529 Size: 35 4825 35 4553 Fragments arranged by size: 4825 4553 35 35 DraI TTT'AAA Cuts at: 6752 6752 Size: 9448 DraIII CAC_nnn'GTG Cuts at: 2593 4767 2593 Size: 2174 7274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3294 8455 809 Size: 507 1978 5161 1802 Fragments arranged by size: 5161 1978 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6348 6895 7784 Size: 54 1425 50 275 18 4518 547 889 Cuts at: 7784 9413 8 Size: 1629 43 Fragments arranged by size: 4518 1629 1425 889 547 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6895 1537 Size: 275 5083 4090 Fragments arranged by size: 5083 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2547 3896 3982 4366 4518 5538 5753 Size: 653 541 1349 86 384 152 1020 215 Cuts at: 5753 6735 7378 1353 Size: 982 643 3423 Fragments arranged by size: 3423 1349 1020 982 653 643 541 384 215 152 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3944 8035 8409 8555 387 Size: 1064 138 2355 4091 374 146 1280 Fragments arranged by size: 4091 2355 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5036 6664 5036 Size: 1628 7820 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2388 2896 3335 3402 3877 4268 5478 Size: 393 756 508 439 67 475 391 1210 Cuts at: 5478 5916 5978 6044 6137 6449 6665 6680 7511 Size: 438 62 66 93 312 216 15 831 Cuts at: 7511 8108 8895 9163 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1210 831 787 756 597 508 475 439 438 393 391 312 268 216 93 67 66 62 15 EcoNI CCTnn'n_nnAGG Cuts at: 4194 6647 6734 4194 Size: 2453 87 6908 Fragments arranged by size: 6908 2453 87 EcoRI G'AATT_C Cuts at: 2110 2348 3154 3422 5692 6757 7135 2110 Size: 238 806 268 2270 1065 378 4423 Fragments arranged by size: 4423 2270 1065 806 378 268 238 EcoRV GAT'ATC Cuts at: 4013 4013 Size: 9448 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4136 6181 4136 Size: 2045 7403 FspI TGC'GCA Cuts at: 4423 7954 4423 Size: 3531 5917 HaeII r_GCGC'y Cuts at: 1678 3416 5096 8595 1678 Size: 1738 1680 3499 2531 Fragments arranged by size: 3499 2531 1738 1680 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 3997 4029 4401 Size: 32 135 32 1022 32 1371 32 372 Cuts at: 4401 4433 4727 4759 5563 5595 6200 6232 6336 Size: 32 294 32 804 32 605 32 104 Cuts at: 6336 6368 8089 8121 8163 8195 1373 Size: 32 1721 32 42 32 2626 Fragments arranged by size: 2626 1721 1371 1022 804 605 372 294 135 104 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2033 3573 6219 6792 7038 669 Size: 988 281 95 1540 2646 573 246 3079 Fragments arranged by size: 3079 2646 1540 988 573 281 246 95 HindIII A'AGCT_T Cuts at: 748 3088 3641 4021 6573 748 Size: 2340 553 380 2552 3623 Fragments arranged by size: 3623 2552 2340 553 380 HpaI GTT'AAC Cuts at: 7038 7038 Size: 9448 KpnI G_GTAC'C Cuts at: 6773 6773 Size: 9448 MfeI C'AATT_G Cuts at: 4425 7047 4425 Size: 2622 6826 MluI A'CGCG_T Cuts at: 9324 9324 Size: 9448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3783 6415 8562 8746 1305 Size: 2478 2632 2147 184 2007 Fragments arranged by size: 2632 2478 2147 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 6350 10 Size: 54 1425 343 4518 3108 Fragments arranged by size: 4518 3108 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2478 4763 5003 5237 5885 7406 7765 Size: 648 1312 2285 240 234 648 1521 359 Cuts at: 7765 7924 518 Size: 159 2042 Fragments arranged by size: 2285 2042 1521 1312 648 648 359 240 234 159 MspA1I CmG'CkG Cuts at: 1606 5733 6115 6864 7541 8689 8934 1606 Size: 4127 382 749 677 1148 245 2120 Fragments arranged by size: 4127 2120 1148 749 677 382 245 NcoI C'CATG_G Cuts at: 513 1065 2187 4530 513 Size: 552 1122 2343 5431 Fragments arranged by size: 5431 2343 1122 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9448 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9448 NotI GC'GGCC_GC Cuts at: 6895 6895 Size: 9448 NsiI A_TGCA'T Cuts at: 2640 2725 3166 4714 2640 Size: 85 441 1548 7374 Fragments arranged by size: 7374 1548 441 85 NspI r_CATG'y Cuts at: 1132 1229 4806 5173 6806 8351 1132 Size: 97 3577 367 1633 1545 2229 Fragments arranged by size: 3577 2229 1633 1545 367 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9448 PciI A'CATG_T Cuts at: 8347 8347 Size: 9448 PflMI CCAn_nnn'nTGG Cuts at: 1108 3818 3907 5915 6316 1108 Size: 2710 89 2008 401 4240 Fragments arranged by size: 4240 2710 2008 401 89 PfoI T'CCnGG_A Cuts at: 4594 6657 4594 Size: 2063 7385 PmeI GTTT'AAAC Cuts at: 6752 6752 Size: 9448 PmlI CAC'GTG Cuts at: 3355 3355 Size: 9448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4341 4373 7517 7549 9030 9062 4341 Size: 32 3144 32 1481 32 4727 Fragments arranged by size: 4727 3144 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9448 PsiI TTA'TAA Cuts at: 2666 2867 4749 5025 6537 7018 2666 Size: 201 1882 276 1512 481 5096 Fragments arranged by size: 5096 1882 1512 481 276 201 PstI C_TGCA'G Cuts at: 830 6108 6800 830 Size: 5278 692 3478 Fragments arranged by size: 5278 3478 692 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9448 PvuII CAG'CTG Cuts at: 5733 6115 5733 Size: 382 9066 SacI G_AGCT'C Cuts at: 5038 6666 5038 Size: 1628 7820 SalI G'TCGA_C Cuts at: 1936 6217 6790 1936 Size: 4281 573 4594 Fragments arranged by size: 4594 4281 573 SapI GCTCTTCn'nnn_ Cuts at: 2547 3982 6735 2547 Size: 1435 2753 5260 Fragments arranged by size: 5260 2753 1435 SbfI CC_TGCA'GG Cuts at: 6800 6800 Size: 9448 ScaI AGT'ACT Cuts at: 7696 9408 7696 Size: 1712 7736 SfcI C'TryA_G Cuts at: 826 1045 6104 6796 7931 8612 8803 826 Size: 219 5059 692 1135 681 191 1471 Fragments arranged by size: 5059 1471 1135 692 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 6266 6266 Size: 9448 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9448 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9448 SmaI CCC'GGG Cuts at: 1872 6775 1872 Size: 4903 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 6098 7552 8453 8715 820 Size: 197 926 2075 2080 1454 901 262 1553 Fragments arranged by size: 2080 2075 1553 1454 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 7239 493 Size: 6746 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9448 SphI G_CATG'C Cuts at: 1132 4806 6806 1132 Size: 3674 2000 3774 Fragments arranged by size: 3774 3674 2000 SspI AAT'ATT Cuts at: 5 52 7372 5 Size: 47 7320 2081 Fragments arranged by size: 7320 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 8203 StyI C'CwwG_G Cuts at: 513 1065 2187 3072 3427 3952 4195 4530 6669 Size: 552 1122 885 355 525 243 335 2139 Cuts at: 6669 6887 513 Size: 218 3074 Fragments arranged by size: 3074 2139 1122 885 552 525 355 335 243 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5028 6323 6357 6674 7475 7492 7645 Size: 1255 3448 1295 34 317 801 17 153 Cuts at: 7645 7830 8275 325 Size: 185 445 1498 Fragments arranged by size: 3448 1498 1295 1255 801 445 317 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3051 4303 4885 Size: 274 80 33 51 1443 1074 1252 582 Cuts at: 4885 4907 5112 5473 6621 7694 9406 96 Size: 22 205 361 1148 1073 1712 138 Fragments arranged by size: 1712 1443 1252 1148 1074 1073 582 361 274 205 138 80 51 33 22 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9408 9440 9408 Size: 32 9416 Tth111I GACn'n_nGTC Cuts at: 1316 6136 1316 Size: 4820 4628 XbaI T'CTAG_A Cuts at: 6784 6784 Size: 9448 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6313 6313 Size: 9448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9448 XmaI C'CCGG_G Cuts at: 1870 6773 1870 Size: 4903 4545 XmnI GAAnn'nnTTC Cuts at: 2984 4826 5655 7577 2984 Size: 1842 829 1922 4855 Fragments arranged by size: 4855 1922 1842 829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 7256 276 Size: 53 83 186 936 1762 3960 2468 Fragments arranged by size: 3960 2468 1762 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI BsmBI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI