(Circular) (Six-base) MAPSORT of: pFN21AB6421.seq Check: 5253 from: 1 to: 5632 pFN21AB6421 5632 bp With 182 enzymes: SgfI * October 20, 2009 11:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2883 3695 5079 5347 1632 Size: 1251 812 1384 268 1917 Fragments arranged by size: 1917 1384 1251 812 268 AflII C'TTAA_G Cuts at: 820 1017 2540 820 Size: 197 1523 3912 Fragments arranged by size: 3912 1523 197 AflIII A'CryG_T Cuts at: 1203 2651 4531 5508 1203 Size: 1448 1880 977 1327 Fragments arranged by size: 1880 1448 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2099 2280 1184 Size: 915 181 4536 Fragments arranged by size: 4536 915 181 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2265 2297 2265 Size: 32 5600 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2314 2432 2600 2904 2941 3167 3319 5428 Size: 821 118 168 304 37 226 152 2109 Cuts at: 5428 1493 Size: 1697 Fragments arranged by size: 2109 1697 821 304 226 168 152 118 37 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2166 2957 1870 Size: 73 186 37 791 4545 Fragments arranged by size: 4545 791 186 73 37 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 2953 4408 618 Size: 325 217 627 248 918 1455 1842 Fragments arranged by size: 1842 1455 918 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2951 1391 Size: 1560 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 928 Size: 971 506 4155 Fragments arranged by size: 4155 971 506 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2100 2134 3823 3857 1903 Size: 34 163 34 1689 34 3678 Fragments arranged by size: 3678 1689 163 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 2539 3693 4849 1790 Size: 749 1154 1156 2573 Fragments arranged by size: 2573 1156 1154 749 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4292 1239 Size: 3053 2579 Bpu10I CC'TnA_GC Cuts at: 2175 2370 2175 Size: 195 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 1186 Size: 961 4671 BseYI C'CCAG_C Cuts at: 2058 4835 5331 2058 Size: 2777 496 2359 Fragments arranged by size: 2777 2359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2120 3082 3843 4871 1540 Size: 275 186 119 962 761 1028 2301 Fragments arranged by size: 2301 1028 962 761 275 186 119 BsiHKAI G_wGCw'C Cuts at: 2718 3693 3778 4849 2718 Size: 975 85 1071 3501 Fragments arranged by size: 3501 1071 975 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2539 2718 2951 3693 3778 4849 1391 Size: 399 749 179 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 749 742 399 233 179 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 2357 794 Size: 1563 4069 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2753 2866 2962 3329 3714 3731 5183 5269 Size: 781 113 96 367 385 17 1452 86 Cuts at: 5269 5281 5627 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 781 385 367 346 113 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2184 513 Size: 552 939 39 141 3961 Fragments arranged by size: 3961 939 552 141 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2358 3148 3942 3962 2118 Size: 240 790 794 20 3788 Fragments arranged by size: 3788 794 790 240 20 ClaI AT'CG_AT Cuts at: 1554 2120 1554 Size: 566 5066 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2149 3079 3968 Size: 54 1425 50 275 18 319 930 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 930 889 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2883 3695 4292 5079 5347 1239 Size: 393 1251 812 597 787 268 1524 Fragments arranged by size: 1524 1251 812 787 597 393 268 EcoRI G'AATT_C Cuts at: 2432 2941 3319 2432 Size: 509 378 4745 Fragments arranged by size: 4745 509 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 5632 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 2822 2854 4273 Size: 32 135 32 1187 32 31 32 1419 Cuts at: 4273 4305 4347 4379 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1419 1187 135 42 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 2488 2548 748 Size: 1740 60 3832 Fragments arranged by size: 3832 1740 60 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2362 2551 4746 4930 1305 Size: 1057 189 2195 184 2007 Fragments arranged by size: 2195 2007 1057 189 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2151 10 Size: 54 1425 343 319 3491 Fragments arranged by size: 3491 1425 343 319 54 MslI CAynn'nnrTG Cuts at: 518 1166 2766 3590 3949 4108 518 Size: 648 1600 824 359 159 2042 Fragments arranged by size: 2042 1600 824 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2512 2593 3048 3725 4873 5118 1606 Size: 906 81 455 677 1148 245 2120 Fragments arranged by size: 2120 1148 906 677 455 245 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2043 2184 513 Size: 552 939 39 141 3961 Fragments arranged by size: 3961 939 552 141 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2417 1108 Size: 1309 4323 PfoI T'CCnGG_A Cuts at: 2661 2661 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 2643 3202 2643 Size: 559 5073 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2120 2001 Size: 119 5513 PvuII CAG'CTG Cuts at: 2512 2512 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2168 2959 1872 Size: 296 791 4545 Fragments arranged by size: 4545 791 296 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3736 4637 4899 820 Size: 197 926 597 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 2402 152 Size: 2250 3382 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 2184 2237 3071 513 Size: 552 939 39 141 53 834 3074 Fragments arranged by size: 3074 939 834 552 141 53 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 5590 96 Size: 274 80 33 51 1443 1901 1712 138 Fragments arranged by size: 1901 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2643 2675 5592 5624 2643 Size: 32 2917 32 2651 Fragments arranged by size: 2917 2651 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2756 2968 2756 Size: 212 5420 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2166 2957 1870 Size: 296 791 4545 Fragments arranged by size: 4545 791 296 XmnI GAAnn'nnTTC Cuts at: 2436 2904 3761 2436 Size: 468 857 4307 Fragments arranged by size: 4307 857 468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI