################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MaeII MamI MboI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AlwNI 422 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 10 GGGCCC GGGCC^C AspI 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 223 CCCGGG C^[CT]CG[GA]G AviII 113 TGCGCA TGC^GCA BalI 471 TGGCCA TGG^CCA BanI 238 GGCGCC G^G[CT][GA]CC BbeI 238 GGCGCC GGCGC^C BbsI 448 GAAGAC GAAGAC(2/6) BpmI 300 CTGGAG CTGGAG(16/14) BpuAI 448 GAAGAC GAAGAC(2/6) BseRI 437 CTCCTC GAGGAG(10/8) BsoBI 223 CCCGGG C^[CT]CG[GA]G BspMI 77 ACCTGC ACCTGC(4/8) BstEII 519 GGTGACC G^GT[ACGT]ACC BstPI 519 GGTGACC G^GT[ACGT]ACC DdeI 542 CTGAG C^T[ACGT]AG DraIII 91 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 76 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 121 CCGCGG C^C[GA][CT]GG EcoNI 378 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 519 GGTGACC G^GT[ACGT]ACC FseI 534 GGCCGGCC GGCCGG^CC FspI 113 TGCGCA TGC^GCA KasI 238 GGCGCC G^GCGCC KspI 121 CCGCGG CCGC^GG MaeIII 520 GTGAC ^GT[ACGT]AC MboII 448 GAAGA GAAGA(8/7) MluNI 471 TGGCCA TGG^CCA MscI 471 TGGCCA TGG^CCA NarI 238 GGCGCC GG^CGCC PflFI 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 90 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 181 GAGTC GAGTC(4/5) PstI 169 CTGCAG CTGCA^G SacII 121 CCGCGG CCGC^GG SfaNI 415 GATGC GCATC(5/9) SfcI 169 CTGCAG C^T[GA][CT]AG SfoI 238 GGCGCC GGC^GCC SmaI 223 CCCGGG CCC^GGG TaqI 74 TCGA T^CGA TfiI 407 GAATC G^A[AT]TC Tsp45I 520 GTGAC ^GT[GC]AC Tth111I 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 74 TCGA T^CGA Van91I 90 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 223 CCCGGG C^CCGGG XmaI 223 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcyI 238 GGCGCC G[GA]^CG[CT]C 364 GACGCC BglI 20 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 199 GCCGGCGAGGC BsaHI 238 GGCGCC G[GA]^CG[CT]C 364 GACGCC BsmAI 356 GAGAC GTCTC(1/5) 399 GTCTC BsrI 264 ACTGG ACTGG(1/-1) 516 ACTGG BstXI 494 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 509 CCACTGAACTGG Hin1I 238 GGCGCC G[GA]^CG[CT]C 364 GACGCC HinfI 181 GAGTC G^A[ACGT]TC 407 GAATC HphI 294 GGTGA GGTGA(8/7) 519 GGTGA MspA1I 121 CCGCGG C[AC]G^C[GT]G 313 CAGCGG NaeI 199 GCCGGC GCC^GGC 535 GCCGGC NgoMIV 199 GCCGGC G^CCGGC 535 GCCGGC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiEI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI TspRI