(Circular) (Six-base) MAPSORT of: pFN21AB7182.seq Check: 6221 from: 1 to: 5257 pFN21AB7182 5257 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3067 278 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2600 1937 Size: 663 4594 Acc65I G'GTAC_C Cuts at: 2578 2578 Size: 5257 AclI AA'CG_TT Cuts at: 1563 3384 3757 1563 Size: 1821 373 3063 Fragments arranged by size: 3063 1821 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3320 4704 4972 1632 Size: 1688 1384 268 1917 Fragments arranged by size: 1917 1688 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5060 AflIII A'CryG_T Cuts at: 1203 4156 5133 1203 Size: 2953 977 1327 Fragments arranged by size: 2953 1327 977 AgeI A'CCGG_T Cuts at: 4088 4088 Size: 5257 AhdI GACnn_n'nnGTC Cuts at: 3986 3986 Size: 5257 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5257 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5257 AlwNI CAG_nnn'CTG Cuts at: 2432 3000 4572 2432 Size: 568 1572 3117 Fragments arranged by size: 3117 1572 568 ApaI G_GGCC'C Cuts at: 2019 2019 Size: 5257 ApaLI G'TGCA_C Cuts at: 3314 4470 3314 Size: 1156 4101 ApoI r'AATT_y Cuts at: 1493 2566 2792 2944 5053 1493 Size: 1073 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 226 152 AseI AT'TA_AT Cuts at: 160 1032 3811 160 Size: 872 2779 1606 Fragments arranged by size: 2779 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 AvaI C'yCGr_G Cuts at: 1870 1943 2228 2582 1870 Size: 73 285 354 4545 Fragments arranged by size: 4545 354 285 73 BamHI G'GATC_C Cuts at: 2587 2954 2587 Size: 367 4890 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 2578 4033 618 Size: 325 217 627 456 335 1455 1842 Fragments arranged by size: 1842 1455 627 456 335 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2115 2382 2513 2576 1391 Size: 628 96 267 131 63 4072 Fragments arranged by size: 4072 628 267 131 96 63 BbeI G_GCGC'C Cuts at: 2247 2247 Size: 5257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 928 Size: 971 561 3725 Fragments arranged by size: 3725 971 561 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3448 3482 1903 Size: 34 1511 34 3678 Fragments arranged by size: 3678 1511 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 4359 3149 Size: 1210 4047 BclI T'GATC_A Cuts at: 1548 1548 Size: 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2031 2210 3868 Size: 107 122 71 631 605 359 179 1658 Cuts at: 3868 136 Size: 1525 Fragments arranged by size: 1658 1525 631 605 359 179 122 107 71 BglII A'GATC_T Cuts at: 5252 5252 Size: 5257 BlpI GC'TnA_GC Cuts at: 2674 2674 Size: 5257 Bme1580I G_kGCm'C Cuts at: 1790 2019 3318 4474 1790 Size: 229 1299 1156 2573 Fragments arranged by size: 2573 1299 1156 229 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5257 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2988 3946 442 Size: 416 53 83 399 24 1571 958 1753 Fragments arranged by size: 1753 1571 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5257 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 5257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2326 3917 1239 Size: 1087 1591 2579 Fragments arranged by size: 2579 1591 1087 Bpu10I CC'TnA_GC Cuts at: 2547 2547 Size: 5257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3382 4247 4545 3382 Size: 865 298 4094 Fragments arranged by size: 4094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3920 882 Size: 617 2421 2219 Fragments arranged by size: 2421 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3048 493 Size: 711 1844 2702 Fragments arranged by size: 2702 1844 711 BsaBI GATnn'nnATC Cuts at: 5251 5251 Size: 5257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2244 2370 3064 3446 Size: 53 83 186 936 711 126 694 382 Cuts at: 3446 4081 275 Size: 635 1451 Fragments arranged by size: 1451 936 711 694 635 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 3690 4088 4362 4509 4920 3690 Size: 398 274 147 411 4027 Fragments arranged by size: 4027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5032 Fragments arranged by size: 5032 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2433 1186 Size: 1247 4010 BseYI C'CCAG_C Cuts at: 2257 2506 4460 4956 2257 Size: 249 1954 496 2558 Fragments arranged by size: 2558 1954 496 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4684 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 2132 2142 2541 2707 3468 Size: 275 186 69 62 10 399 166 761 Cuts at: 3468 4496 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 399 275 186 166 69 62 10 BsiHKAI G_wGCw'C Cuts at: 3318 3403 4474 3318 Size: 85 1071 4101 Fragments arranged by size: 4101 1071 85 BsmI GAATG_Cn' Cuts at: 2768 2861 2768 Size: 93 5164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2115 2382 2513 2576 3318 3403 Size: 399 229 96 267 131 63 742 85 Cuts at: 3403 4474 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 229 131 96 85 63 BspEI T'CCGG_A Cuts at: 4920 4920 Size: 5257 BspHI T'CATG_A Cuts at: 3146 4876 3146 Size: 1730 3527 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2612 844 Size: 1247 521 3489 Fragments arranged by size: 3489 1247 521 BsrBI CCG'CTC Cuts at: 1394 1823 3144 1394 Size: 429 1321 3507 Fragments arranged by size: 3507 1321 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3752 3926 65 Size: 1183 2504 174 1396 Fragments arranged by size: 2504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2204 2540 3901 4088 1700 Size: 110 327 67 336 1361 187 2869 Fragments arranged by size: 2869 1361 336 327 187 110 67 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5257 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5257 BssSI C'ACGA_G Cuts at: 3317 4329 3317 Size: 1012 4245 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5257 BstBI TT'CG_AA Cuts at: 4061 4061 Size: 5257 BstEII G'GTnAC_C Cuts at: 1184 2524 1184 Size: 1340 3917 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2521 2506 Size: 15 5242 BstYI r'GATC_y Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252 Size: 615 367 385 17 1452 86 12 346 Cuts at: 5252 1972 Size: 1977 Fragments arranged by size: 1977 1452 615 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2126 513 Size: 552 1061 3644 Fragments arranged by size: 3644 1061 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4518 Fragments arranged by size: 4518 683 56 BtsI GCAGTG_nn' Cuts at: 2095 2773 3567 3587 2095 Size: 678 794 20 3765 Fragments arranged by size: 3765 794 678 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5222 DraI TTT'AAA Cuts at: 2561 2561 Size: 5257 DraIII CAC_nnn'GTG Cuts at: 2101 2101 Size: 5257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2087 4264 809 Size: 507 771 2177 1802 Fragments arranged by size: 2177 1802 771 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2129 2139 Size: 54 1425 50 275 18 237 62 10 Cuts at: 2139 2476 2538 2704 3593 5222 8 Size: 337 62 166 889 1629 43 Fragments arranged by size: 1629 1425 889 337 275 237 166 62 62 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2067 2129 2139 2538 2704 1537 Size: 275 255 62 10 399 166 4090 Fragments arranged by size: 4090 399 275 255 166 62 10 EarI CTCTTCn'nnn_ Cuts at: 1353 3187 1353 Size: 1834 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 3844 4218 4364 387 Size: 1064 138 492 1763 374 146 1280 Fragments arranged by size: 1763 1280 1064 492 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2326 3320 3917 4704 4972 1239 Size: 393 694 994 597 787 268 1524 Fragments arranged by size: 1524 994 787 694 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2387 2387 Size: 5257 EcoO109I rG'GnC_Cy Cuts at: 2015 2016 2350 2015 Size: 1 334 4922 Fragments arranged by size: 4922 334 1 EcoRI G'AATT_C Cuts at: 2566 2944 2566 Size: 378 4879 FseI GG_CCGG'CC Cuts at: 2544 2544 Size: 5257 FspI TGC'GCA Cuts at: 2120 3763 2120 Size: 1643 3614 HaeII r_GCGC'y Cuts at: 1678 2174 2219 2247 2282 4404 1678 Size: 496 45 28 35 2122 2531 Fragments arranged by size: 2531 2122 496 45 35 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373 Size: 32 135 32 2326 32 42 32 2626 Fragments arranged by size: 2626 2326 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 2847 669 Size: 988 281 663 246 3079 Fragments arranged by size: 3079 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5257 HpaI GTT'AAC Cuts at: 2847 2847 Size: 5257 KasI G'GCGC_C Cuts at: 2243 2243 Size: 5257 KpnI G_GTAC'C Cuts at: 2582 2582 Size: 5257 MfeI C'AATT_G Cuts at: 2856 2856 Size: 5257 MluI A'CGCG_T Cuts at: 5133 5133 Size: 5257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4371 4555 1305 Size: 3066 184 2007 Fragments arranged by size: 3066 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2478 10 Size: 54 1425 343 646 2789 Fragments arranged by size: 2789 1425 646 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3215 3574 3733 518 Size: 648 2049 359 159 2042 Fragments arranged by size: 2049 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2320 2673 3350 4498 4743 1606 Size: 522 192 353 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 522 353 245 192 NaeI GCC'GGC Cuts at: 2206 2542 2206 Size: 336 4921 NarI GG'CG_CC Cuts at: 2244 2244 Size: 5257 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4705 NdeI CA'TA_TG Cuts at: 387 387 Size: 5257 NgoMIV G'CCGG_C Cuts at: 2204 2540 2204 Size: 336 4921 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5257 NotI GC'GGCC_GC Cuts at: 2704 2704 Size: 5257 NspI r_CATG'y Cuts at: 1132 1229 2615 4160 1132 Size: 97 1386 1545 2229 Fragments arranged by size: 2229 1545 1386 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5257 PciI A'CATG_T Cuts at: 4156 4156 Size: 5257 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 1108 Size: 993 4264 PfoI T'CCnGG_A Cuts at: 2407 2407 Size: 5257 PmeI GTTT'AAAC Cuts at: 2561 2561 Size: 5257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3326 3358 4839 4871 3326 Size: 32 1481 32 3712 Fragments arranged by size: 3712 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5257 PsiI TTA'TAA Cuts at: 2827 2827 Size: 5257 PspOMI G'GGCC_C Cuts at: 2015 2015 Size: 5257 PstI C_TGCA'G Cuts at: 830 2178 2609 830 Size: 1348 431 3478 Fragments arranged by size: 3478 1348 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5257 SacII CC_GC'GG Cuts at: 2129 2129 Size: 5257 SalI G'TCGA_C Cuts at: 1936 2599 1936 Size: 663 4594 SbfI CC_TGCA'GG Cuts at: 2609 2609 Size: 5257 ScaI AGT'ACT Cuts at: 3505 5217 3505 Size: 1712 3545 SfcI C'TryA_G Cuts at: 826 1045 2174 2605 3740 4421 4612 826 Size: 219 1129 431 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 681 431 219 191 SfoI GGC'GCC Cuts at: 2245 2245 Size: 5257 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5257 SmaI CCC'GGG Cuts at: 1872 2230 2584 1872 Size: 358 354 4545 Fragments arranged by size: 4545 358 354 SmlI C'TyrA_G Cuts at: 820 1017 1943 3361 4262 4524 820 Size: 197 926 1418 901 262 1553 Fragments arranged by size: 1553 1418 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3048 493 Size: 2555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5257 SphI G_CATG'C Cuts at: 1132 2615 1132 Size: 1483 3774 SrfI GCCC'GGGC Cuts at: 2230 2230 Size: 5257 SspI AAT'ATT Cuts at: 5 52 3181 5 Size: 47 3129 2081 Fragments arranged by size: 3129 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5257 StyI C'CwwG_G Cuts at: 513 1065 2696 513 Size: 552 1631 3074 Fragments arranged by size: 3074 1631 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3284 3301 3454 3639 4084 325 Size: 1255 1704 17 153 185 445 1498 Fragments arranged by size: 1704 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3503 5215 96 Size: 274 80 33 51 1443 1526 1712 138 Fragments arranged by size: 1712 1526 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5217 5249 5217 Size: 32 5225 Tth111I GACn'n_nGTC Cuts at: 1316 2390 1316 Size: 1074 4183 XbaI T'CTAG_A Cuts at: 2593 2593 Size: 5257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5257 XmaI C'CCGG_G Cuts at: 1870 2228 2582 1870 Size: 358 354 4545 Fragments arranged by size: 4545 358 354 XmnI GAAnn'nnTTC Cuts at: 3386 3386 Size: 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3065 276 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI