(Circular) (Six-base) MAPSORT of: pFN21AE0292.seq Check: 1422 from: 1 to: 5461 pFN21AE0292 5461 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2305 2305 Size: 5461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 2804 1937 Size: 556 311 4594 Fragments arranged by size: 4594 556 311 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2710 3524 4908 5176 1632 Size: 1078 814 1384 268 1917 Fragments arranged by size: 1917 1384 1078 814 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 4190 4190 Size: 5461 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2624 1184 Size: 1440 4021 AlwNI CAG_nnn'CTG Cuts at: 2623 3204 4776 2623 Size: 581 1572 3308 Fragments arranged by size: 3308 1572 581 ApaLI G'TGCA_C Cuts at: 2084 2513 3518 4674 2084 Size: 429 1005 1156 2871 Fragments arranged by size: 2871 1156 1005 429 ApoI r'AATT_y Cuts at: 1493 2770 2996 3148 5257 1493 Size: 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2124 2175 2280 2786 1870 Size: 73 117 64 51 105 506 4545 Fragments arranged by size: 4545 506 117 105 73 64 51 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 2330 2410 2444 2482 Size: 325 217 627 489 54 80 34 38 Cuts at: 2482 2782 4237 618 Size: 300 1455 1842 Fragments arranged by size: 1842 1455 627 489 325 300 217 80 54 38 34 BanII G_rGCy'C Cuts at: 1391 2780 1391 Size: 1389 4072 BbeI G_GCGC'C Cuts at: 2280 2334 2448 2280 Size: 54 114 5293 Fragments arranged by size: 5293 114 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4490 BbvCI CC'TCA_GC Cuts at: 2501 2501 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2074 2292 2301 Size: 107 122 71 631 605 402 218 9 Cuts at: 2301 4072 136 Size: 1771 1525 Fragments arranged by size: 1771 1525 631 605 402 218 122 107 71 9 BglII A'GATC_T Cuts at: 5456 5456 Size: 5461 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2088 2415 2487 2517 3522 4678 1790 Size: 298 327 72 30 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 327 298 72 30 BmgBI CAC'GTC Cuts at: 1318 2345 1318 Size: 1027 4434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3192 4150 442 Size: 416 53 83 399 24 1775 958 1753 Fragments arranged by size: 1775 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4121 1239 Size: 2882 2579 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2528 2703 4124 882 Size: 617 1029 175 1421 2219 Fragments arranged by size: 2219 1421 1029 617 175 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 5455 5455 Size: 5461 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2232 2277 2331 2445 Size: 53 83 186 936 699 45 54 114 Cuts at: 2445 2490 2649 3268 3650 4285 275 Size: 45 159 619 382 635 1451 Fragments arranged by size: 1451 936 699 635 619 382 186 159 114 83 54 53 45 45 BsaWI w'CCGG_w Cuts at: 3894 4292 4566 4713 5124 3894 Size: 398 274 147 411 4231 Fragments arranged by size: 4231 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2065 2095 1178 Size: 30 165 30 662 30 4544 Fragments arranged by size: 4544 662 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5461 BseYI C'CCAG_C Cuts at: 2591 4664 5160 2591 Size: 2073 496 2892 Fragments arranged by size: 2892 2073 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 2461 2528 1163 Size: 573 633 92 67 4096 Fragments arranged by size: 4096 633 573 92 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2574 2680 2751 2911 3672 4700 Size: 275 186 573 106 71 160 761 1028 Cuts at: 4700 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 573 275 186 160 106 71 BsiHKAI G_wGCw'C Cuts at: 2088 2517 2580 2706 3522 3607 4678 2088 Size: 429 63 126 816 85 1071 2871 Fragments arranged by size: 2871 1071 816 429 126 85 63 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2415 2487 2517 2580 2706 2780 Size: 399 298 327 72 30 63 126 74 Cuts at: 2780 3522 3607 4678 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 327 298 126 85 74 72 63 30 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 2550 2816 844 Size: 1461 245 266 3489 Fragments arranged by size: 3489 1461 266 245 BsrBI CCG'CTC Cuts at: 1394 1823 2256 3348 1394 Size: 429 433 1092 3507 Fragments arranged by size: 3507 1092 433 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 2246 2370 2559 2746 4105 4292 Size: 110 418 18 124 189 187 1359 187 Cuts at: 4292 1700 Size: 2869 Fragments arranged by size: 2869 1359 418 189 187 187 124 110 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5461 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 794 Size: 1757 3704 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456 Size: 819 367 385 17 1452 86 12 346 Cuts at: 5456 1972 Size: 1977 Fragments arranged by size: 1977 1452 819 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 2364 2460 2568 513 Size: 552 939 42 318 96 108 3406 Fragments arranged by size: 3406 939 552 318 108 96 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2086 2977 3771 3791 2033 Size: 53 891 794 20 3703 Fragments arranged by size: 3703 891 794 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2457 2571 2731 Size: 54 1425 50 275 18 627 114 160 Cuts at: 2731 2748 2908 3797 5426 8 Size: 17 160 889 1629 43 Fragments arranged by size: 1629 1425 889 627 275 160 160 114 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2571 2748 2908 1537 Size: 275 759 177 160 4090 Fragments arranged by size: 4090 759 275 177 160 EarI CTCTTCn'nnn_ Cuts at: 1353 3391 1353 Size: 2038 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 4422 4568 387 Size: 1064 138 2459 374 146 1280 Fragments arranged by size: 2459 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2710 3524 4121 4908 5176 1239 Size: 393 1078 814 597 787 268 1524 Fragments arranged by size: 1524 1078 814 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2052 2076 2140 2155 2284 2380 2052 Size: 24 64 15 129 96 5133 Fragments arranged by size: 5133 129 96 64 24 15 EcoRI G'AATT_C Cuts at: 2770 3148 2770 Size: 378 5083 FspI TGC'GCA Cuts at: 2663 3967 2663 Size: 1304 4157 FspAI rTGC'GCAy Cuts at: 2663 2663 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2280 2334 2448 4608 1678 Size: 602 54 114 2160 2531 Fragments arranged by size: 2531 2160 602 114 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4102 4134 4176 4208 1373 Size: 32 135 32 2530 32 42 32 2626 Fragments arranged by size: 2626 2530 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3051 669 Size: 988 281 867 246 3079 Fragments arranged by size: 3079 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KasI G'GCGC_C Cuts at: 2276 2330 2444 2276 Size: 54 114 5293 Fragments arranged by size: 5293 114 54 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2418 4575 4759 1305 Size: 1113 2157 184 2007 Fragments arranged by size: 2157 2007 1113 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3639 Fragments arranged by size: 3639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 2570 2730 2877 3554 4702 4947 1606 Size: 856 108 160 147 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 245 160 147 108 NaeI GCC'GGC Cuts at: 2230 2248 2372 2748 2230 Size: 18 124 376 4943 Fragments arranged by size: 4943 376 124 18 NarI GG'CG_CC Cuts at: 2277 2331 2445 2277 Size: 54 114 5293 Fragments arranged by size: 5293 114 54 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NgoMIV G'CCGG_C Cuts at: 2228 2246 2370 2746 2228 Size: 18 124 376 4943 Fragments arranged by size: 4943 376 124 18 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2668 2819 4364 1132 Size: 97 1439 151 1545 2229 Fragments arranged by size: 2229 1545 1439 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2155 2140 Size: 15 5446 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PspXI vC'TCGA_Gb Cuts at: 2175 2175 Size: 5461 PstI C_TGCA'G Cuts at: 830 2292 2813 830 Size: 1462 521 3478 Fragments arranged by size: 3478 1462 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SacII CC_GC'GG Cuts at: 2463 2571 2463 Size: 108 5353 SalI G'TCGA_C Cuts at: 1936 2803 1936 Size: 867 4594 SbfI CC_TGCA'GG Cuts at: 2292 2813 2292 Size: 521 4940 ScaI AGT'ACT Cuts at: 3709 5421 3709 Size: 1712 3749 SexAI A'CCwGG_T Cuts at: 2736 2736 Size: 5461 SfcI C'TryA_G Cuts at: 826 1045 2190 2288 2809 3944 4625 4816 826 Size: 219 1145 98 521 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 681 521 219 191 98 SfiI GGCCn_nnn'nGGCC Cuts at: 2292 2301 2292 Size: 9 5452 SfoI GGC'GCC Cuts at: 2278 2332 2446 2278 Size: 54 114 5293 Fragments arranged by size: 5293 114 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2062 2126 2282 2788 1872 Size: 190 64 156 506 4545 Fragments arranged by size: 4545 506 190 156 64 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 3565 4466 4728 820 Size: 197 926 232 1390 901 262 1553 Fragments arranged by size: 1553 1390 926 901 262 232 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2819 1132 Size: 1687 3774 SrfI GCCC'GGGC Cuts at: 2062 2062 Size: 5461 SspI AAT'ATT Cuts at: 5 52 3385 5 Size: 47 3333 2081 Fragments arranged by size: 3333 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5461 StyI C'CwwG_G Cuts at: 513 1065 2004 2900 513 Size: 552 939 896 3074 Fragments arranged by size: 3074 939 896 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 3488 3505 3658 3843 4288 325 Size: 1255 1067 841 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1067 841 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3707 5419 96 Size: 274 80 33 51 1443 1730 1712 138 Fragments arranged by size: 1730 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2065 2097 5421 5453 2065 Size: 32 3324 32 2073 Fragments arranged by size: 3324 2073 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2651 1316 Size: 1335 4126 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 2175 1943 Size: 232 5229 XmaI C'CCGG_G Cuts at: 1870 2060 2124 2280 2786 1870 Size: 190 64 156 506 4545 Fragments arranged by size: 4545 506 190 156 64 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SanDI SapI SwaI XcmI