(Circular) (Six-base) MAPSORT of: pFN21AE0687.seq Check: 9223 from: 1 to: 5989 pFN21AE0687 5989 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2977 4052 5436 5704 1632 Size: 1345 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1345 1075 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 2375 1166 Size: 1209 4780 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 4606 AlwNI CAG_nnn'CTG Cuts at: 3732 5304 3732 Size: 1572 4417 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 2726 3298 3524 3676 5785 1493 Size: 1233 572 226 152 2109 1697 Fragments arranged by size: 2109 1697 1233 572 226 152 AseI AT'TA_AT Cuts at: 160 1032 2696 4543 160 Size: 872 1664 1847 1606 Fragments arranged by size: 1847 1664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2451 2833 3314 1870 Size: 73 120 388 382 481 4545 Fragments arranged by size: 4545 481 388 382 120 73 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2369 2502 2551 3277 3310 Size: 325 217 627 582 133 49 726 33 Cuts at: 3310 4765 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 726 627 582 325 217 133 49 33 BanII G_rGCy'C Cuts at: 1391 2070 2850 3308 1391 Size: 679 780 458 4072 Fragments arranged by size: 4072 780 679 458 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5018 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2932 2966 4180 4214 1903 Size: 34 995 34 1214 34 3678 Fragments arranged by size: 3678 1214 995 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3011 4600 136 Size: 107 122 71 631 605 1339 1589 1525 Fragments arranged by size: 1589 1525 1339 631 605 122 107 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 2165 3406 2165 Size: 1241 4748 Bme1580I G_kGCm'C Cuts at: 1790 2372 4050 5206 1790 Size: 582 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 582 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3720 4678 442 Size: 416 53 83 399 24 2303 958 1753 Fragments arranged by size: 2303 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2739 1056 Size: 1683 4306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2864 4649 1239 Size: 1625 1785 2579 Fragments arranged by size: 2579 1785 1625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2300 4114 4979 5277 2300 Size: 1814 865 298 3012 Fragments arranged by size: 3012 1814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 2987 5983 2987 Size: 2996 2993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2892 4422 4820 5094 5241 5652 2892 Size: 1530 398 274 147 411 3229 Fragments arranged by size: 3229 1530 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3011 3041 3074 3104 1178 Size: 30 165 30 1608 30 33 30 4063 Fragments arranged by size: 4063 1608 165 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2234 3062 3079 3224 3227 1186 Size: 854 194 828 17 145 3 3948 Fragments arranged by size: 3948 854 828 194 145 17 3 BseYI C'CCAG_C Cuts at: 2173 5192 5688 2173 Size: 3019 496 2474 Fragments arranged by size: 3019 2474 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2153 1163 Size: 573 417 4999 Fragments arranged by size: 4999 573 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2850 4050 4135 5206 2850 Size: 1200 85 1071 3633 Fragments arranged by size: 3633 1200 1071 85 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 BsmBI CGTCTCn'nnnn_ Cuts at: 2072 2072 Size: 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2372 2850 3308 4050 4135 5206 Size: 399 280 302 478 458 742 85 1071 Cuts at: 5206 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 458 399 302 280 85 BspEI T'CCGG_A Cuts at: 2892 5652 2892 Size: 2760 3229 BspHI T'CATG_A Cuts at: 2520 3120 3878 5608 2520 Size: 600 758 1730 2901 Fragments arranged by size: 2901 1730 758 600 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 3344 844 Size: 1979 521 3489 Fragments arranged by size: 3489 1979 521 BsrBI CCG'CTC Cuts at: 1394 1823 3876 1394 Size: 429 2053 3507 Fragments arranged by size: 3507 2053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2864 4633 4820 1700 Size: 110 1054 1769 187 2869 Fragments arranged by size: 2869 1769 1054 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 2227 1184 Size: 1043 4946 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2920 2011 Size: 909 5080 BstYI r'GATC_y Cuts at: 1972 3319 3686 4071 4088 5540 5626 5638 5984 Size: 1347 367 385 17 1452 86 12 346 Cuts at: 5984 1972 Size: 1977 Fragments arranged by size: 1977 1452 1347 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2817 487 Size: 683 56 1591 3659 Fragments arranged by size: 3659 1591 683 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2922 3150 3436 Size: 54 1425 50 275 18 1092 228 286 Cuts at: 3436 4325 5954 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1092 889 286 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2285 2972 3919 1353 Size: 932 687 947 3423 Fragments arranged by size: 3423 947 932 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3125 4576 4950 5096 387 Size: 1064 138 1536 1451 374 146 1280 Fragments arranged by size: 1536 1451 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2848 2848 Size: 5989 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2864 2977 4052 4649 5436 5704 1239 Size: 393 1232 113 1075 597 787 268 1524 Fragments arranged by size: 1524 1232 1075 787 597 393 268 113 EcoO109I rG'GnC_Cy Cuts at: 2829 2829 Size: 5989 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2340 3000 3040 5136 1678 Size: 662 660 40 2096 2531 Fragments arranged by size: 2531 2096 662 660 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3011 3043 4630 4662 4704 Size: 32 135 32 1439 32 1587 32 42 Cuts at: 4704 4736 1373 Size: 32 2626 Fragments arranged by size: 2626 1587 1439 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2017 3100 3333 3579 669 Size: 988 281 79 1083 233 246 3079 Fragments arranged by size: 3079 1083 988 281 246 233 79 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5103 5287 1305 Size: 3798 184 2007 Fragments arranged by size: 3798 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2924 3152 10 Size: 54 1425 343 1092 228 2847 Fragments arranged by size: 2847 1425 1092 343 228 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2195 2375 3267 3947 4306 4465 Size: 648 843 186 180 892 680 359 159 Cuts at: 4465 518 Size: 2042 Fragments arranged by size: 2042 892 843 680 648 359 186 180 159 MspA1I CmG'CkG Cuts at: 1606 2129 2784 2857 3405 4082 5230 5475 1606 Size: 523 655 73 548 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 655 548 523 245 73 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 2689 3264 387 Size: 2302 575 3112 Fragments arranged by size: 3112 2302 575 NheI G'CTAG_C Cuts at: 1052 2735 1052 Size: 1683 4306 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2290 2947 3347 4892 1132 Size: 97 1061 657 400 1545 2229 Fragments arranged by size: 2229 1545 1061 657 400 97 PasI CC'CwG_GG Cuts at: 1348 3185 1348 Size: 1837 4152 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 2118 3006 1108 Size: 1010 888 4091 Fragments arranged by size: 4091 1010 888 PfoI T'CCnGG_A Cuts at: 2665 2665 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2052 2084 4058 4090 5571 5603 2052 Size: 32 1974 32 1481 32 2438 Fragments arranged by size: 2438 1974 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 3341 830 Size: 2511 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2857 2857 Size: 5989 SacI G_AGCT'C Cuts at: 2850 2850 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SexAI A'CCwGG_T Cuts at: 2498 2498 Size: 5989 SfcI C'TryA_G Cuts at: 826 1045 3337 4472 5153 5344 826 Size: 219 2292 1135 681 191 1471 Fragments arranged by size: 2292 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 2453 3316 1872 Size: 581 863 4545 Fragments arranged by size: 4545 863 581 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 4093 4994 5256 820 Size: 197 926 372 1778 901 262 1553 Fragments arranged by size: 1778 1553 926 901 372 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 2290 2947 3347 1132 Size: 1158 657 400 3774 Fragments arranged by size: 3774 1158 657 400 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 2004 3428 513 Size: 552 939 1424 3074 Fragments arranged by size: 3074 1424 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2796 4016 4033 4186 4371 4816 325 Size: 1255 1216 1220 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1220 1216 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 4235 5947 Size: 274 80 33 51 1443 168 2090 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 2090 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5949 5981 5949 Size: 32 5957 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 2413 3325 2413 Size: 912 5077 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2362 2362 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 2451 3314 1870 Size: 581 863 4545 Fragments arranged by size: 4545 863 581 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI