(Circular) (Six-base) MAPSORT of: pFN21AE0678.seq Check: 8365 from: 1 to: 5893 pFN21AE0678 5893 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2346 2346 Size: 5893 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 1937 Size: 1299 4594 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 5340 5608 1632 Size: 2324 1384 268 1917 Fragments arranged by size: 2324 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 2289 2712 3038 4792 5769 1203 Size: 1086 423 326 1754 977 1327 Fragments arranged by size: 1754 1327 1086 977 423 326 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 2623 4622 2623 Size: 1999 3894 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 2276 Size: 32 5861 AlwNI CAG_nnn'CTG Cuts at: 2236 3636 5208 2236 Size: 1400 1572 2921 Fragments arranged by size: 2921 1572 1400 ApaLI G'TGCA_C Cuts at: 2483 3950 5106 2483 Size: 1467 1156 3270 Fragments arranged by size: 3270 1467 1156 ApoI r'AATT_y Cuts at: 1493 2076 3202 3428 3580 5689 1493 Size: 583 1126 226 152 2109 1697 Fragments arranged by size: 2109 1697 1126 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3218 1870 Size: 73 1275 4545 Fragments arranged by size: 4545 1275 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 2566 3214 4669 618 Size: 325 217 627 762 17 648 1455 1842 Fragments arranged by size: 1842 1455 762 648 627 325 217 17 BanII G_rGCy'C Cuts at: 1391 3212 1391 Size: 1821 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2200 2292 3035 928 Size: 971 301 92 743 3786 Fragments arranged by size: 3786 971 743 301 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 2481 2487 3954 5110 1790 Size: 691 6 1467 1156 2573 Fragments arranged by size: 2573 1467 1156 691 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2869 3624 4582 Size: 416 53 83 399 24 1452 755 958 Cuts at: 4582 442 Size: 1753 Fragments arranged by size: 1753 1452 958 755 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2258 2569 2668 4553 1239 Size: 965 54 311 99 1885 2579 Fragments arranged by size: 2579 1885 965 311 99 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2963 4018 4883 5181 2963 Size: 1055 865 298 3675 Fragments arranged by size: 3675 1055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2316 2659 4556 882 Size: 617 817 343 1897 2219 Fragments arranged by size: 2219 1897 817 617 343 BsaAI yAC'GTr Cuts at: 493 1204 2482 3684 493 Size: 711 1278 1202 2702 Fragments arranged by size: 2702 1278 1202 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 4082 4717 275 Size: 53 83 186 936 2167 382 635 1451 Fragments arranged by size: 2167 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4326 4724 4998 5145 5556 4326 Size: 398 274 147 411 4663 Fragments arranged by size: 4663 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5668 Fragments arranged by size: 5668 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5893 BseYI C'CCAG_C Cuts at: 2314 5096 5592 2314 Size: 2782 496 2615 Fragments arranged by size: 2782 2615 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 2487 3057 3954 4039 5110 2244 Size: 243 570 897 85 1071 3027 Fragments arranged by size: 3027 1071 897 570 243 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 BsmBI CGTCTCn'nnnn_ Cuts at: 2714 2714 Size: 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2481 2487 3057 3212 3954 4039 Size: 399 454 237 6 570 155 742 85 Cuts at: 4039 5110 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 570 454 399 237 155 85 6 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 2346 2421 3248 844 Size: 1490 12 75 827 3489 Fragments arranged by size: 3489 1490 827 75 12 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2160 2197 2502 4388 4562 65 Size: 1183 912 37 305 1886 174 1396 Fragments arranged by size: 1886 1396 1183 912 305 174 37 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 2122 2707 96 Size: 2026 585 3282 Fragments arranged by size: 3282 2026 585 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstYI r'GATC_y Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888 Size: 1251 367 385 17 1452 86 12 346 Cuts at: 5888 1972 Size: 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2541 513 Size: 552 939 537 3865 Fragments arranged by size: 3865 939 552 537 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2525 487 Size: 683 56 1299 3855 Fragments arranged by size: 3855 1299 683 56 BtsI GCAGTG_nn' Cuts at: 3409 4203 4223 3409 Size: 794 20 5079 Fragments arranged by size: 5079 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DraIII CAC_nnn'GTG Cuts at: 3167 3167 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858 Size: 54 1425 50 275 18 1510 889 1629 Cuts at: 5858 8 Size: 43 Fragments arranged by size: 1629 1510 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 2873 3823 1353 Size: 1031 489 950 3423 Fragments arranged by size: 3423 1031 950 489 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2258 2569 2668 3956 4553 5340 Size: 393 572 54 311 99 1288 597 787 Cuts at: 5340 5608 1239 Size: 268 1524 Fragments arranged by size: 1524 1288 787 597 572 393 311 268 99 54 EcoNI CCTnn'n_nnAGG Cuts at: 2907 2907 Size: 5893 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3074 3074 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2656 2688 4534 Size: 32 135 32 1037 32 15 32 1846 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1846 1037 135 42 32 32 32 32 32 32 15 HincII GTy'rAC Cuts at: 669 1657 1938 2059 2617 3237 3483 669 Size: 988 281 121 558 620 246 3079 Fragments arranged by size: 3079 988 620 558 281 246 121 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5007 5191 1305 Size: 3702 184 2007 Fragments arranged by size: 3702 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2300 3851 4210 4369 518 Size: 648 1128 6 1551 359 159 2042 Fragments arranged by size: 2042 1551 1128 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 3309 3986 5134 5379 1606 Size: 1703 677 1148 245 2120 Fragments arranged by size: 2120 1703 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2541 513 Size: 552 939 537 3865 Fragments arranged by size: 3865 939 552 537 NdeI CA'TA_TG Cuts at: 387 2644 387 Size: 2257 3636 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2293 3042 3251 4796 1132 Size: 97 1064 749 209 1545 2229 Fragments arranged by size: 2229 1545 1064 749 209 97 PasI CC'CwG_GG Cuts at: 1348 2520 2629 1348 Size: 1172 109 4612 Fragments arranged by size: 4612 1172 109 PciI A'CATG_T Cuts at: 2289 3038 4792 2289 Size: 749 1754 3390 Fragments arranged by size: 3390 1754 749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PmlI CAC'GTG Cuts at: 2482 2482 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 2418 3245 830 Size: 1588 827 3478 Fragments arranged by size: 3478 1588 827 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SapI GCTCTTCn'nnn_ Cuts at: 2384 2384 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 2689 4141 5853 2689 Size: 1452 1712 2729 Fragments arranged by size: 2729 1712 1452 SfcI C'TryA_G Cuts at: 826 1045 2414 3241 4376 5057 5248 826 Size: 219 1369 827 1135 681 191 1471 Fragments arranged by size: 1471 1369 1135 827 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 3997 4898 5160 820 Size: 197 926 999 1055 901 262 1553 Fragments arranged by size: 1553 1055 999 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 2458 3817 5 Size: 47 2406 1359 2081 Fragments arranged by size: 2406 2081 1359 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2004 2541 3141 3332 513 Size: 552 939 537 600 191 3074 Fragments arranged by size: 3074 939 600 552 537 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 3920 3937 4090 4275 4720 325 Size: 1255 726 1614 17 153 185 445 1498 Fragments arranged by size: 1614 1498 1255 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2122 2160 2687 Size: 274 80 33 51 1443 145 38 527 Cuts at: 2687 2707 4139 5851 96 Size: 20 1432 1712 138 Fragments arranged by size: 1712 1443 1432 527 274 145 138 80 51 38 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 3065 1316 Size: 1749 4144 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 2378 3077 4022 2378 Size: 699 945 4249 Fragments arranged by size: 4249 945 699 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI