Restriction map for
pFN21AE0678
(Linear) (Six-base) MAPSORT of: KIEE0678.seq Check: 9547 from: 1 to: 1188
KIEE0678.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 1188
Size: 341 847
AflIII A'CryG_T
Cuts at: 0 284 707 1033 1188
Size: 284 423 326 155
Fragments arranged by size:
423 326 284 155
AhdI GACnn_n'nnGTC
Cuts at: 0 618 1188
Size: 618 570
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 1188
Size: 271 32 885
Fragments arranged by size:
885 271 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 1188
Size: 231 957
ApaLI G'TGCA_C
Cuts at: 0 478 1188
Size: 478 710
ApoI r'AATT_y
Cuts at: 0 71 1188
Size: 71 1117
BanI G'GyrC_C
Cuts at: 0 544 561 1188
Size: 544 17 627
Fragments arranged by size:
627 544 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 287 1030 1188
Size: 195 92 743 158
Fragments arranged by size:
743 195 158 92
Bme1580I G_kGCm'C
Cuts at: 0 476 482 1188
Size: 476 6 706
Fragments arranged by size:
706 476 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 864 1188
Size: 864 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 253 564 663 1188
Size: 199 54 311 99 525
Fragments arranged by size:
525 311 199 99 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 1188
Size: 958 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 311 654 1188
Size: 311 343 534
Fragments arranged by size:
534 343 311
BsaAI yAC'GTr
Cuts at: 0 477 1188
Size: 477 711
BseYI C'CCAG_C
Cuts at: 0 309 1188
Size: 309 879
BsiHKAI G_wGCw'C
Cuts at: 0 239 482 1052 1188
Size: 239 243 570 136
Fragments arranged by size:
570 243 239 136
BsmBI CGTCTCn'nnnn_
Cuts at: 0 709 1188
Size: 709 479
Bsp1286I G_dGCh'C
Cuts at: 0 239 476 482 1052 1188
Size: 239 237 6 570 136
Fragments arranged by size:
570 239 237 136 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 341 416 1188
Size: 329 12 75 772
Fragments arranged by size:
772 329 75 12
BsrDI GCAATG_nn'
Cuts at: 0 155 192 497 1188
Size: 155 37 305 691
Fragments arranged by size:
691 305 155 37
BsrGI T'GTAC_A
Cuts at: 0 117 702 1188
Size: 117 585 486
Fragments arranged by size:
585 486 117
BtgI C'CryG_G
Cuts at: 0 536 1188
Size: 536 652
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 520 1188
Size: 520 668
DraIII CAC_nnn'GTG
Cuts at: 0 1162 1188
Size: 1162 26
EarI CTCTTCn'nnn_
Cuts at: 0 379 868 1188
Size: 379 489 320
Fragments arranged by size:
489 379 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 253 564 663 1188
Size: 199 54 311 99 525
Fragments arranged by size:
525 311 199 99 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 902 1188
Size: 902 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1069 1188
Size: 1069 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 651 683 1188
Size: 604 32 15 32 505
Fragments arranged by size:
604 505 32 32 15
HincII GTy'rAC
Cuts at: 0 54 612 1188
Size: 54 558 576
Fragments arranged by size:
576 558 54
MslI CAynn'nnrTG
Cuts at: 0 289 295 1188
Size: 289 6 893
Fragments arranged by size:
893 289 6
NcoI C'CATG_G
Cuts at: 0 536 1188
Size: 536 652
NdeI CA'TA_TG
Cuts at: 0 639 1188
Size: 639 549
NspI r_CATG'y
Cuts at: 0 288 1037 1188
Size: 288 749 151
Fragments arranged by size:
749 288 151
PasI CC'CwG_GG
Cuts at: 0 515 624 1188
Size: 515 109 564
Fragments arranged by size:
564 515 109
PciI A'CATG_T
Cuts at: 0 284 1033 1188
Size: 284 749 155
Fragments arranged by size:
749 284 155
PmlI CAC'GTG
Cuts at: 0 477 1188
Size: 477 711
PstI C_TGCA'G
Cuts at: 0 413 1188
Size: 413 775
SapI GCTCTTCn'nnn_
Cuts at: 0 379 1188
Size: 379 809
ScaI AGT'ACT
Cuts at: 0 684 1188
Size: 684 504
SfcI C'TryA_G
Cuts at: 0 409 1188
Size: 409 779
SmlI C'TyrA_G
Cuts at: 0 937 1188
Size: 937 251
SspI AAT'ATT
Cuts at: 0 453 1188
Size: 453 735
StyI C'CwwG_G
Cuts at: 0 536 1136 1188
Size: 536 600 52
Fragments arranged by size:
600 536 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1188
Size: 301 887
TatI w'GTAC_w
Cuts at: 0 117 155 682 702 1188
Size: 117 38 527 20 486
Fragments arranged by size:
527 486 117 38 20
Tth111I GACn'n_nGTC
Cuts at: 0 1060 1188
Size: 1060 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1188
Size: 555 633
XmnI GAAnn'nnTTC
Cuts at: 0 373 1072 1188
Size: 373 699 116
Fragments arranged by size:
699 373 116
Enzymes that do cut:
AarI AflIII AhdI AloI AlwNI ApaLI ApoI BanI
BbsI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BseYI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BtgI BtgZI
DraIII EarI Eco57MI EcoNI FalI Hin4I HincII MslI
NcoI NdeI NspI PasI PciI PmlI PstI SapI
ScaI SfcI SmlI SspI StyI TaqII TatI Tth111I
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI XbaI XhoI
XmaI ZraI