Restriction map for
pFN21AB8532
(Linear) (Six-base) MAPSORT of: KIBB8532.seq Check: 9664 from: 1 to: 2694
KIBB8532.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 0 449 2694
Size: 449 2245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1255 1433 2694
Size: 688 567 178 1261
Fragments arranged by size:
1261 688 567 178
AfeI AGC'GCT
Cuts at: 0 1672 2694
Size: 1672 1022
AgeI A'CCGG_T
Cuts at: 0 2184 2694
Size: 2184 510
AleI CACnn'nnGTG
Cuts at: 0 715 2694
Size: 715 1979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 868 2694
Size: 733 135 1826
Fragments arranged by size:
1826 733 135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 2572 2604 2694
Size: 88 32 2452 32 90
Fragments arranged by size:
2452 90 88 32 32
ApaI G_GGCC'C
Cuts at: 0 1277 1343 2694
Size: 1277 66 1351
Fragments arranged by size:
1351 1277 66
ApaLI G'TGCA_C
Cuts at: 0 708 2694
Size: 708 1986
ApoI r'AATT_y
Cuts at: 0 14 1849 2428 2694
Size: 14 1835 579 266
Fragments arranged by size:
1835 579 266 14
AvaI C'yCGr_G
Cuts at: 0 751 1425 2250 2389 2592 2694
Size: 751 674 825 139 203 102
Fragments arranged by size:
825 751 674 203 139 102
AvrII C'CTAG_G
Cuts at: 0 999 2694
Size: 999 1695
BamHI G'GATC_C
Cuts at: 0 2404 2694
Size: 2404 290
BanI G'GyrC_C
Cuts at: 0 1987 2187 2694
Size: 1987 200 507
Fragments arranged by size:
1987 507 200
BanII G_rGCy'C
Cuts at: 0 758 1196 1277 1343 1628 1640 1824 2299
Size: 758 438 81 66 285 12 184 475
Cuts at: 2299 2694
Size: 395
Fragments arranged by size:
758 475 438 395 285 184 81 66
12
BbeI G_GCGC'C
Cuts at: 0 1991 2694
Size: 1991 703
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 428 2694
Size: 247 181 2266
Fragments arranged by size:
2266 247 181
BbvCI CC'TCA_GC
Cuts at: 0 1448 2694
Size: 1448 1246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 473 1308 1342 2694
Size: 439 34 835 34 1352
Fragments arranged by size:
1352 835 439 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2459 2694
Size: 2459 235
BlpI GC'TnA_GC
Cuts at: 0 1859 2694
Size: 1859 835
Bme1580I G_kGCm'C
Cuts at: 0 203 712 722 1277 1343 1913 2192 2694
Size: 203 509 10 555 66 570 279 502
Fragments arranged by size:
570 555 509 502 279 203 66 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1048 1280 1483 1588 1819 2694
Size: 1048 232 203 105 231 875
Fragments arranged by size:
1048 875 232 231 203 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 2694
Size: 1659 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 349 575 847 2694
Size: 103 246 226 272 1847
Fragments arranged by size:
1847 272 246 226 103
Bpu10I CC'TnA_GC
Cuts at: 0 1448 2478 2694
Size: 1448 1030 216
Fragments arranged by size:
1448 1030 216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 717 1612 2694
Size: 295 422 895 1082
Fragments arranged by size:
1082 895 422 295
BsaI GGTCTCn'nnnn_
Cuts at: 0 1447 1642 2694
Size: 1447 195 1052
Fragments arranged by size:
1447 1052 195
BsaAI yAC'GTr
Cuts at: 0 1368 2694
Size: 1368 1326
BsaHI Gr'CG_yC
Cuts at: 0 622 838 1988 2694
Size: 622 216 1150 706
Fragments arranged by size:
1150 706 622 216
BsaWI w'CCGG_w
Cuts at: 0 2184 2694
Size: 2184 510
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1126 1156 2359 2389 2572 2602 2694
Size: 1126 30 1203 30 183 30 92
Fragments arranged by size:
1203 1126 183 92 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 1138 1150 1195 1465 2130 2532 2694
Size: 91 1047 12 45 270 665 402 162
Fragments arranged by size:
1047 665 402 270 162 91 45 12
BseYI C'CCAG_C
Cuts at: 0 229 1147 2020 2203 2623 2694
Size: 229 918 873 183 420 71
Fragments arranged by size:
918 873 420 229 183 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1348 2694
Size: 1348 1346
BsiHKAI G_wGCw'C
Cuts at: 0 251 712 758 1640 2694
Size: 251 461 46 882 1054
Fragments arranged by size:
1054 882 461 251 46
BsmI GAATG_Cn'
Cuts at: 0 51 1215 2016 2094 2109 2694
Size: 51 1164 801 78 15 585
Fragments arranged by size:
1164 801 585 78 51 15
Bsp1286I G_dGCh'C
Cuts at: 0 203 251 712 722 758 1196 1277 1343
Size: 203 48 461 10 36 438 81 66
Cuts at: 1343 1628 1640 1824 1913 2192 2299 2694
Size: 285 12 184 89 279 107 395
Fragments arranged by size:
461 438 395 285 279 203 184 107
89 81 66 48 36 12 10
BsrBI CCG'CTC
Cuts at: 0 117 2694
Size: 117 2577
BsrFI r'CCGG_y
Cuts at: 0 2014 2184 2694
Size: 2014 170 510
Fragments arranged by size:
2014 510 170
BstAPI GCAn_nnn'nTGC
Cuts at: 0 963 2694
Size: 963 1731
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 1717 2694
Size: 1305 412 977
Fragments arranged by size:
1305 977 412
BstYI r'GATC_y
Cuts at: 0 324 1073 2404 2694
Size: 324 749 1331 290
Fragments arranged by size:
1331 749 324 290
BtgI C'CryG_G
Cuts at: 0 1995 2346 2694
Size: 1995 351 348
Fragments arranged by size:
1995 351 348
BtsI GCAGTG_nn'
Cuts at: 0 961 1478 1538 2694
Size: 961 517 60 1156
Fragments arranged by size:
1156 961 517 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2421 2456 2694
Size: 2421 35 238
Fragments arranged by size:
2421 238 35
DraIII CAC_nnn'GTG
Cuts at: 0 949 2694
Size: 949 1745
EaeI y'GGCC_r
Cuts at: 0 383 395 782 981 1355 1370 2601 2694
Size: 383 12 387 199 374 15 1231 93
Fragments arranged by size:
1231 387 383 374 199 93 15 12
EarI CTCTTCn'nnn_
Cuts at: 0 1609 1810 1935 2694
Size: 1609 201 125 759
Fragments arranged by size:
1609 759 201 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2417 2537 2694
Size: 2417 120 157
Fragments arranged by size:
2417 157 120
EcoICRI GAG'CTC
Cuts at: 0 756 1638 2694
Size: 756 882 1056
Fragments arranged by size:
1056 882 756
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 349 575 688 847 1255 1433 2694
Size: 103 246 226 113 159 408 178 1261
Fragments arranged by size:
1261 408 246 226 178 159 113 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 376 2694
Size: 310 66 2318
Fragments arranged by size:
2318 310 66
EcoO109I rG'GnC_Cy
Cuts at: 0 300 702 730 810 1340 1891 2694
Size: 300 402 28 80 530 551 803
Fragments arranged by size:
803 551 530 402 300 80 28
EcoRV GAT'ATC
Cuts at: 0 939 2694
Size: 939 1755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 336 2694
Size: 336 2358
FspI TGC'GCA
Cuts at: 0 953 2694
Size: 953 1741
HaeII r_GCGC'y
Cuts at: 0 124 1674 1991 2694
Size: 124 1550 317 703
Fragments arranged by size:
1550 703 317 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 2694
Size: 1126 32 1536
Fragments arranged by size:
1536 1126 32
HincII GTy'rAC
Cuts at: 0 450 573 2694
Size: 450 123 2121
Fragments arranged by size:
2121 450 123
HindIII A'AGCT_T
Cuts at: 0 1771 2694
Size: 1771 923
KasI G'GCGC_C
Cuts at: 0 1987 2694
Size: 1987 707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 161 654 1748 2432 2694
Size: 91 70 493 1094 684 262
Fragments arranged by size:
1094 684 493 262 91 70
MscI TGG'CCA
Cuts at: 0 397 2694
Size: 397 2297
MslI CAynn'nnrTG
Cuts at: 0 196 715 850 1303 2694
Size: 196 519 135 453 1391
Fragments arranged by size:
1391 519 453 196 135
MspA1I CmG'CkG
Cuts at: 0 1233 2694
Size: 1233 1461
NarI GG'CG_CC
Cuts at: 0 1988 2694
Size: 1988 706
NcoI C'CATG_G
Cuts at: 0 1995 2346 2694
Size: 1995 351 348
Fragments arranged by size:
1995 351 348
PasI CC'CwG_GG
Cuts at: 0 2500 2694
Size: 2500 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 99 2694
Size: 42 57 2595
Fragments arranged by size:
2595 57 42
PfoI T'CCnGG_A
Cuts at: 0 371 2694
Size: 371 2323
PmlI CAC'GTG
Cuts at: 0 1368 2694
Size: 1368 1326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2572 2604 2694
Size: 2572 32 90
Fragments arranged by size:
2572 90 32
PpuMI rG'GwC_Cy
Cuts at: 0 702 730 2694
Size: 702 28 1964
Fragments arranged by size:
1964 702 28
PspOMI G'GGCC_C
Cuts at: 0 1273 1339 2694
Size: 1273 66 1355
Fragments arranged by size:
1355 1273 66
PspXI vC'TCGA_Gb
Cuts at: 0 1425 2694
Size: 1425 1269
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2671 2694
Size: 2671 23
PstI C_TGCA'G
Cuts at: 0 1250 2694
Size: 1250 1444
PvuII CAG'CTG
Cuts at: 0 1233 2694
Size: 1233 1461
SacI G_AGCT'C
Cuts at: 0 758 1640 2694
Size: 758 882 1054
Fragments arranged by size:
1054 882 758
SalI G'TCGA_C
Cuts at: 0 448 2694
Size: 448 2246
SanDI GG'GwC_CC
Cuts at: 0 730 2694
Size: 730 1964
SfcI C'TryA_G
Cuts at: 0 1246 2067 2079 2301 2436 2610 2694
Size: 1246 821 12 222 135 174 84
Fragments arranged by size:
1246 821 222 174 135 84 12
SfoI GGC'GCC
Cuts at: 0 1989 2694
Size: 1989 705
SgrAI Cr'CCGG_yG
Cuts at: 0 2184 2694
Size: 2184 510
SmaI CCC'GGG
Cuts at: 0 2391 2594 2694
Size: 2391 203 100
Fragments arranged by size:
2391 203 100
SmlI C'TyrA_G
Cuts at: 0 310 696 751 1425 1627 2694
Size: 310 386 55 674 202 1067
Fragments arranged by size:
1067 674 386 310 202 55
StuI AGG'CCT
Cuts at: 0 1333 1744 2694
Size: 1333 411 950
Fragments arranged by size:
1333 950 411
StyI C'CwwG_G
Cuts at: 0 617 999 1710 1995 2163 2346 2550 2694
Size: 617 382 711 285 168 183 204 144
Fragments arranged by size:
711 617 382 285 204 183 168 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 787 807 1471 1860 2694
Size: 787 20 664 389 834
Fragments arranged by size:
834 787 664 389 20
TatI w'GTAC_w
Cuts at: 0 1250 2687 2694
Size: 1250 1437 7
Fragments arranged by size:
1437 1250 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 714 746 2094 2126 2694
Size: 499 32 183 32 1348 32 568
Fragments arranged by size:
1348 568 499 183 32 32 32
XbaI T'CTAG_A
Cuts at: 0 915 2694
Size: 915 1779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 1365 1731 2611 2694
Size: 792 573 366 880 83
Fragments arranged by size:
880 792 573 366 83
XhoI C'TCGA_G
Cuts at: 0 751 1425 2694
Size: 751 674 1269
Fragments arranged by size:
1269 751 674
XmaI C'CCGG_G
Cuts at: 0 2389 2592 2694
Size: 2389 203 102
Fragments arranged by size:
2389 203 102
Enzymes that do cut:
AccI AcuI AfeI AgeI AleI AlfI AloI ApaI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrFI
BstAPI BstXI BstYI BtgI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NarI NcoI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuII SacI
SalI SanDI SfcI SfoI SgrAI SmaI SmlI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AlwNI
AscI AseI AsiSI BaeI BciVI BclI BfrBI BglII
BmgBI BmtI BsaBI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EagI EcoRI FseI
FspAI HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PshAI PsiI PvuI RsrII SacII SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XmnI ZraI