(Circular) (Six-base) MAPSORT of: pFN21AB6622.seq Check: 4920 from: 1 to: 6013 pFN21AB6622 6013 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3162 4076 5460 5728 1632 Size: 1530 914 1384 268 1917 Fragments arranged by size: 1917 1530 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 2143 820 Size: 197 1126 4690 Fragments arranged by size: 4690 1126 197 AflIII A'CryG_T Cuts at: 1203 2582 4912 5889 1203 Size: 1379 2330 977 1327 Fragments arranged by size: 2330 1379 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 2145 1166 Size: 979 5034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4351 AlwNI CAG_nnn'CTG Cuts at: 3756 5328 3756 Size: 1572 4441 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2070 2483 2659 3184 3322 3548 3700 5809 Size: 577 413 176 525 138 226 152 2109 Cuts at: 5809 1493 Size: 1697 Fragments arranged by size: 2109 1697 577 525 413 226 176 152 138 AseI AT'TA_AT Cuts at: 160 1032 3155 4567 160 Size: 872 2123 1412 1606 Fragments arranged by size: 2123 1606 1412 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2208 3338 1870 Size: 73 265 1130 4545 Fragments arranged by size: 4545 1130 265 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 4789 618 Size: 325 217 627 1547 1455 1842 Fragments arranged by size: 1842 1547 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3332 1391 Size: 1941 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2905 6008 2905 Size: 3103 2910 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 4074 5230 1790 Size: 2284 1156 2573 Fragments arranged by size: 2573 2284 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2456 3744 4702 Size: 416 53 83 399 24 1039 1288 958 Cuts at: 4702 442 Size: 1753 Fragments arranged by size: 1753 1288 1039 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4673 1239 Size: 3434 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4676 882 Size: 617 3177 2219 Fragments arranged by size: 3177 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2078 3804 493 Size: 711 874 1726 2702 Fragments arranged by size: 2702 1726 874 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 4202 4837 275 Size: 53 83 186 936 2287 382 635 1451 Fragments arranged by size: 2287 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 2370 2400 1178 Size: 30 165 30 709 30 228 30 4791 Fragments arranged by size: 4791 709 228 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6013 BseYI C'CCAG_C Cuts at: 5216 5712 5216 Size: 496 5517 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2535 1163 Size: 573 799 4641 Fragments arranged by size: 4641 799 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4074 4159 5230 4074 Size: 85 1071 4857 Fragments arranged by size: 4857 1071 85 BsmI GAATG_Cn' Cuts at: 3247 3524 3617 3247 Size: 277 93 5643 Fragments arranged by size: 5643 277 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3332 4074 4159 5230 1391 Size: 399 1542 742 85 1071 2174 Fragments arranged by size: 2174 1542 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3120 3902 5632 3120 Size: 782 1730 3501 Fragments arranged by size: 3501 1730 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 2838 3368 844 Size: 1878 116 530 3489 Fragments arranged by size: 3489 1878 530 116 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3003 4508 4682 65 Size: 1183 1755 1505 174 1396 Fragments arranged by size: 1755 1505 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2640 4657 4844 1700 Size: 110 830 2017 187 2869 Fragments arranged by size: 2869 2017 830 187 110 BsrGI T'GTAC_A Cuts at: 96 2956 96 Size: 2860 3153 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6013 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 2441 1184 Size: 1257 4756 BstYI r'GATC_y Cuts at: 1972 2905 3343 3710 4095 4112 5564 5650 5662 Size: 933 438 367 385 17 1452 86 12 Cuts at: 5662 6008 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 933 438 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2173 3165 513 Size: 552 1108 992 3361 Fragments arranged by size: 3361 1108 992 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2865 487 Size: 683 56 1639 3635 Fragments arranged by size: 3635 1639 683 56 BtsI GCAGTG_nn' Cuts at: 3290 3529 4323 4343 3290 Size: 239 794 20 4960 Fragments arranged by size: 4960 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 2433 2695 2865 3009 3317 2433 Size: 262 170 144 308 5129 Fragments arranged by size: 5129 308 262 170 144 DraIII CAC_nnn'GTG Cuts at: 2049 2049 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978 Size: 54 1425 50 275 18 1630 889 1629 Cuts at: 5978 8 Size: 43 Fragments arranged by size: 1630 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3021 3943 1353 Size: 1668 922 3423 Fragments arranged by size: 3423 1668 922 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3162 4076 4673 5460 5728 1239 Size: 393 1530 914 597 787 268 1524 Fragments arranged by size: 1530 1524 914 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 6013 EcoRI G'AATT_C Cuts at: 2659 3322 3700 2659 Size: 663 378 4972 Fragments arranged by size: 4972 663 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2259 2259 Size: 6013 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4654 4686 4728 4760 1373 Size: 32 135 32 3082 32 42 32 2626 Fragments arranged by size: 3082 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 2395 2427 748 Size: 1647 32 4334 Fragments arranged by size: 4334 1647 32 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2356 5127 5311 1305 Size: 1051 2771 184 2007 Fragments arranged by size: 2771 2007 1051 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2301 3009 3971 4330 4489 518 Size: 648 979 156 708 962 359 159 2042 Fragments arranged by size: 2042 979 962 708 648 359 159 156 MspA1I CmG'CkG Cuts at: 1606 3205 3429 4106 5254 5499 1606 Size: 1599 224 677 1148 245 2120 Fragments arranged by size: 2120 1599 1148 677 245 224 NcoI C'CATG_G Cuts at: 513 1065 2173 3165 513 Size: 552 1108 992 3361 Fragments arranged by size: 3361 1108 992 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2586 2848 3371 4916 1132 Size: 97 1357 262 523 1545 2229 Fragments arranged by size: 2229 1545 1357 523 262 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 2582 4912 2582 Size: 2330 3683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 2990 3099 3583 2990 Size: 109 484 5420 Fragments arranged by size: 5420 484 109 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2277 2309 2277 Size: 32 5981 PstI C_TGCA'G Cuts at: 830 2316 2719 3252 3365 830 Size: 1486 403 533 113 3478 Fragments arranged by size: 3478 1486 533 403 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 PvuII CAG'CTG Cuts at: 3205 3205 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SapI GCTCTTCn'nnn_ Cuts at: 3021 3021 Size: 6013 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 2799 4261 5973 2799 Size: 1462 1712 2839 Fragments arranged by size: 2839 1712 1462 SfcI C'TryA_G Cuts at: 826 1045 2312 2715 2801 3248 3270 3361 4496 Size: 219 1267 403 86 447 22 91 1135 Cuts at: 4496 5177 5368 826 Size: 681 191 1471 Fragments arranged by size: 1471 1267 1135 681 447 403 219 191 91 86 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2143 4117 5018 5280 820 Size: 197 926 200 1974 901 262 1553 Fragments arranged by size: 1974 1553 926 901 262 200 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 2691 2953 3937 5 Size: 47 2639 262 984 2081 Fragments arranged by size: 2639 2081 984 262 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2173 3165 3452 513 Size: 552 1108 992 287 3074 Fragments arranged by size: 3074 1108 992 552 287 SwaI ATTT'AAAT Cuts at: 3009 3009 Size: 6013 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2460 2901 4040 4057 4210 4395 4840 Size: 1255 880 441 1139 17 153 185 445 Cuts at: 4840 325 Size: 1498 Fragments arranged by size: 1498 1255 1139 880 445 441 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2128 2797 2956 Size: 274 80 33 51 1443 151 669 159 Cuts at: 2956 4259 5971 96 Size: 1303 1712 138 Fragments arranged by size: 1712 1443 1303 669 274 159 151 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2760 2810 4142 2760 Size: 50 1332 4631 Fragments arranged by size: 4631 1332 50 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI XcmI