(Circular) (Six-base) MAPSORT of: pFN21AB5754.seq Check: 839 from: 1 to: 6952 pFN21AB5754 6952 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4295 1937 Size: 2358 4594 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3875 3928 5015 6399 6667 1632 Size: 2243 53 1087 1384 268 1917 Fragments arranged by size: 2243 1917 1384 1087 268 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 5851 6828 1203 Size: 4648 977 1327 Fragments arranged by size: 4648 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 2867 5681 2867 Size: 2814 4138 AleI CACnn'nnGTG Cuts at: 1166 2090 1166 Size: 924 6028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6952 AlwNI CAG_nnn'CTG Cuts at: 3587 4695 6267 3587 Size: 1108 1572 4272 Fragments arranged by size: 4272 1572 1108 ApaLI G'TGCA_C Cuts at: 5009 6165 5009 Size: 1156 5796 ApoI r'AATT_y Cuts at: 1493 2231 2652 3047 3116 3593 4261 4487 4639 Size: 738 421 395 69 477 668 226 152 Cuts at: 4639 6748 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 738 668 477 421 395 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 2757 5506 160 Size: 872 1725 2749 1606 Fragments arranged by size: 2749 1725 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 4277 1870 Size: 73 2334 4545 Fragments arranged by size: 4545 2334 73 BamHI G'GATC_C Cuts at: 2242 4282 4649 2242 Size: 2040 367 4545 Fragments arranged by size: 4545 2040 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4273 5728 618 Size: 325 217 627 2486 1455 1842 Fragments arranged by size: 2486 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3660 4271 1391 Size: 2269 611 4072 Fragments arranged by size: 4072 2269 611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2474 2990 4168 928 Size: 971 575 516 1178 3712 Fragments arranged by size: 3712 1178 971 575 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5143 5177 1903 Size: 34 3206 34 3678 Fragments arranged by size: 3678 3206 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3687 4844 6054 3687 Size: 1157 1210 4585 Fragments arranged by size: 4585 1210 1157 BclI T'GATC_A Cuts at: 1548 2458 1548 Size: 910 6042 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5563 136 Size: 107 122 71 631 605 3891 1525 Fragments arranged by size: 3891 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 4369 4369 Size: 6952 Bme1580I G_kGCm'C Cuts at: 1790 5013 6169 1790 Size: 3223 1156 2573 Fragments arranged by size: 3223 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3458 1318 Size: 2140 4812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4683 5641 442 Size: 416 53 83 399 24 3266 958 1753 Fragments arranged by size: 3266 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 3945 5612 1239 Size: 873 1833 1667 2579 Fragments arranged by size: 2579 1833 1667 873 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5077 5942 6240 5077 Size: 865 298 5789 Fragments arranged by size: 5789 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 3685 5615 882 Size: 617 717 1469 1930 2219 Fragments arranged by size: 2219 1930 1469 717 617 BsaAI yAC'GTr Cuts at: 493 1204 2081 2558 4743 493 Size: 711 877 477 2185 2702 Fragments arranged by size: 2702 2185 877 711 477 BsaBI GATnn'nnATC Cuts at: 2247 6946 2247 Size: 4699 2253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4759 5141 5776 275 Size: 53 83 186 936 3226 382 635 1451 Fragments arranged by size: 3226 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5385 5783 6057 6204 6615 5385 Size: 398 274 147 411 5722 Fragments arranged by size: 5722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 2258 2288 1178 Size: 30 165 30 630 30 195 30 5842 Fragments arranged by size: 5842 630 195 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2765 1186 Size: 1579 5373 BseYI C'CCAG_C Cuts at: 3461 3484 6155 6651 3461 Size: 23 2671 496 3762 Fragments arranged by size: 3762 2671 496 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2538 2952 1163 Size: 573 802 414 5163 Fragments arranged by size: 5163 802 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 5013 5098 6169 2046 Size: 2967 85 1071 2829 Fragments arranged by size: 2967 2829 1071 85 BsmI GAATG_Cn' Cuts at: 2548 4070 4463 4556 2548 Size: 1522 393 93 4944 Fragments arranged by size: 4944 1522 393 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 3660 4271 5013 5098 6169 1391 Size: 399 256 1614 611 742 85 1071 2174 Fragments arranged by size: 2174 1614 1071 742 611 399 256 85 BspEI T'CCGG_A Cuts at: 6615 6615 Size: 6952 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3678 4307 844 Size: 2834 629 3489 Fragments arranged by size: 3489 2834 629 BsrBI CCG'CTC Cuts at: 1394 1823 4839 1394 Size: 429 3016 3507 Fragments arranged by size: 3507 3016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5447 5621 65 Size: 1183 4199 174 1396 Fragments arranged by size: 4199 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5596 5783 1700 Size: 110 3786 187 2869 Fragments arranged by size: 3786 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6952 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 5012 6024 5012 Size: 1012 5940 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6952 BstBI TT'CG_AA Cuts at: 5756 5756 Size: 6952 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstYI r'GATC_y Cuts at: 1972 2017 2131 2242 3583 4282 4649 5034 5051 Size: 45 114 111 1341 699 367 385 17 Cuts at: 5051 6503 6589 6601 6947 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1341 699 385 367 346 114 111 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2607 4137 513 Size: 552 1542 1530 3328 Fragments arranged by size: 3328 1542 1530 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6213 Fragments arranged by size: 6213 683 56 BtsI GCAGTG_nn' Cuts at: 3193 4468 5262 5282 3193 Size: 1275 794 20 4863 Fragments arranged by size: 4863 1275 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 3874 4256 3874 Size: 382 6570 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5959 809 Size: 507 4643 1802 Fragments arranged by size: 4643 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3930 4399 5288 Size: 54 1425 50 275 18 2100 469 889 Cuts at: 5288 6917 8 Size: 1629 43 Fragments arranged by size: 2100 1629 1425 889 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3981 4882 1353 Size: 818 1810 901 3423 Fragments arranged by size: 3423 1810 901 818 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5539 5913 6059 387 Size: 1064 138 3950 374 146 1280 Fragments arranged by size: 3950 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 3875 3928 3945 5015 5612 6399 Size: 393 480 1763 53 17 1070 597 787 Cuts at: 6399 6667 1239 Size: 268 1524 Fragments arranged by size: 1763 1524 1070 787 597 480 393 268 53 17 EcoNI CCTnn'n_nnAGG Cuts at: 3699 3699 Size: 6952 EcoO109I rG'GnC_Cy Cuts at: 2103 2103 Size: 6952 EcoRI G'AATT_C Cuts at: 2652 4261 4639 2652 Size: 1609 378 4965 Fragments arranged by size: 4965 1609 378 EcoRV GAT'ATC Cuts at: 2884 2884 Size: 6952 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2122 2536 3829 2122 Size: 414 1293 5245 Fragments arranged by size: 5245 1293 414 FspI TGC'GCA Cuts at: 5458 5458 Size: 6952 HaeII r_GCGC'y Cuts at: 1678 6099 1678 Size: 4421 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2853 2885 3308 3340 4013 Size: 32 135 32 1281 32 423 32 673 Cuts at: 4013 4045 5593 5625 5667 5699 1373 Size: 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 1281 673 423 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2709 4296 4542 669 Size: 988 281 771 1587 246 3079 Fragments arranged by size: 3079 1587 988 771 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3374 6066 6250 1305 Size: 2069 2692 184 2007 Fragments arranged by size: 2692 2069 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3932 10 Size: 54 1425 343 2100 3030 Fragments arranged by size: 3030 2100 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2090 2390 4121 4910 5269 5428 Size: 648 870 54 300 1731 789 359 159 Cuts at: 5428 518 Size: 2042 Fragments arranged by size: 2042 1731 870 789 648 359 300 159 54 MspA1I CmG'CkG Cuts at: 1606 3575 4368 5045 6193 6438 1606 Size: 1969 793 677 1148 245 2120 Fragments arranged by size: 2120 1969 1148 793 677 245 NcoI C'CATG_G Cuts at: 513 1065 2607 4137 513 Size: 552 1542 1530 3328 Fragments arranged by size: 3328 1542 1530 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6952 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 2119 4310 5855 1132 Size: 97 890 2191 1545 2229 Fragments arranged by size: 2229 2191 1545 890 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6952 PciI A'CATG_T Cuts at: 5851 5851 Size: 6952 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6952 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PmlI CAC'GTG Cuts at: 2558 2558 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5021 5053 6534 6566 5021 Size: 32 1481 32 5407 Fragments arranged by size: 5407 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3753 1613 Size: 2140 4812 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PstI C_TGCA'G Cuts at: 830 4304 830 Size: 3474 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 3575 3575 Size: 6952 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SexAI A'CCwGG_T Cuts at: 2696 2696 Size: 6952 SfcI C'TryA_G Cuts at: 826 1045 3576 4300 5435 6116 6307 826 Size: 219 2531 724 1135 681 191 1471 Fragments arranged by size: 2531 1471 1135 724 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 4279 1872 Size: 2407 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5056 5957 6219 820 Size: 197 926 3113 901 262 1553 Fragments arranged by size: 3113 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4310 1132 Size: 3178 3774 SspI AAT'ATT Cuts at: 5 52 3274 4876 5 Size: 47 3222 1602 2081 Fragments arranged by size: 3222 2081 1602 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6952 StyI C'CwwG_G Cuts at: 513 1065 2607 3417 3971 4137 4391 513 Size: 552 1542 810 554 166 254 3074 Fragments arranged by size: 3074 1542 810 554 552 254 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3164 4979 4996 5149 5334 5779 325 Size: 1255 1584 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1584 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3951 5198 6910 Size: 274 80 33 51 1443 1974 1247 1712 Cuts at: 6910 96 Size: 138 Fragments arranged by size: 1974 1712 1443 1247 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 6912 6944 2036 Size: 32 4844 32 2044 Fragments arranged by size: 4844 2044 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6952 XbaI T'CTAG_A Cuts at: 2398 4288 2398 Size: 1890 5062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 2293 Size: 6952 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 4277 1870 Size: 2407 4545 XmnI GAAnn'nnTTC Cuts at: 2394 2471 5081 2394 Size: 77 2610 4265 Fragments arranged by size: 4265 2610 77 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI