(Circular) (Six-base) MAPSORT of: pFN21AB0437.seq Check: 7165 from: 1 to: 6148 pFN21AB0437 6148 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 2618 3469 2618 Size: 851 5297 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2841 2858 2907 2942 3143 3341 4211 5595 Size: 1209 17 49 35 201 198 870 1384 Cuts at: 5595 5863 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1209 870 268 201 198 49 35 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 2826 5047 6024 1203 Size: 1623 2221 977 1327 Fragments arranged by size: 2221 1623 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 3078 3110 2318 Size: 32 728 32 5356 Fragments arranged by size: 5356 728 32 32 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2460 2834 3044 3457 3683 3835 5944 1493 Size: 967 374 210 413 226 152 2109 1697 Fragments arranged by size: 2109 1697 967 413 374 226 210 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2143 3473 1870 Size: 73 200 1330 4545 Fragments arranged by size: 4545 1330 200 73 BamHI G'GATC_C Cuts at: 2364 3478 3845 2364 Size: 1114 367 4667 Fragments arranged by size: 4667 1114 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 3380 3469 4924 618 Size: 325 217 627 831 762 89 1455 1842 Fragments arranged by size: 1842 1455 831 762 627 325 217 89 BanII G_rGCy'C Cuts at: 1391 2425 2641 2882 3055 3467 1391 Size: 1034 216 241 173 412 4072 Fragments arranged by size: 4072 1034 412 241 216 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 4247 Fragments arranged by size: 4247 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3025 4040 5250 3025 Size: 1015 1210 3923 Fragments arranged by size: 3923 1210 1015 BclI T'GATC_A Cuts at: 1548 3210 1548 Size: 1662 4486 BfrBI ATG'CAT Cuts at: 3018 3018 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2842 3324 6143 2842 Size: 482 2819 2847 Fragments arranged by size: 2847 2819 482 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 3383 4209 5365 1790 Size: 1593 826 1156 2573 Fragments arranged by size: 2573 1593 1156 826 BmgBI CAC'GTC Cuts at: 1318 2827 2907 1318 Size: 1509 80 4559 Fragments arranged by size: 4559 1509 80 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 3386 3879 Size: 416 53 83 399 24 758 1211 493 Cuts at: 3879 4837 442 Size: 958 1753 Fragments arranged by size: 1753 1211 958 758 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2439 3111 3387 4808 1239 Size: 866 334 672 276 1421 2579 Fragments arranged by size: 2579 1421 866 672 334 276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4273 5138 5436 4273 Size: 865 298 4985 Fragments arranged by size: 4985 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2994 4811 882 Size: 617 1495 1817 2219 Fragments arranged by size: 2219 1817 1495 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3955 4337 4972 275 Size: 53 83 186 936 2422 382 635 1451 Fragments arranged by size: 2422 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2048 2063 2078 1178 Size: 30 165 30 630 15 15 15 5248 Fragments arranged by size: 5248 630 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2108 2948 1186 Size: 897 25 840 4386 Fragments arranged by size: 4386 897 840 25 BseYI C'CCAG_C Cuts at: 5351 5847 5351 Size: 496 5652 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2239 2464 2709 1163 Size: 573 503 225 245 4602 Fragments arranged by size: 4602 573 503 245 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2641 3055 3082 4209 4294 5365 2641 Size: 414 27 1127 85 1071 3424 Fragments arranged by size: 3424 1127 1071 414 85 27 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2425 2641 2882 3055 3082 3383 3467 Size: 399 635 216 241 173 27 301 84 Cuts at: 3467 4209 4294 5365 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 635 399 301 241 216 173 85 84 27 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 3503 844 Size: 2299 360 3489 Fragments arranged by size: 3489 2299 360 BsrBI CCG'CTC Cuts at: 1394 1823 4035 1394 Size: 429 2212 3507 Fragments arranged by size: 3507 2212 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2677 2944 4643 4817 65 Size: 1183 1429 267 1699 174 1396 Fragments arranged by size: 1699 1429 1396 1183 267 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3353 4792 4979 1700 Size: 110 1543 1439 187 2869 Fragments arranged by size: 2869 1543 1439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstYI r'GATC_y Cuts at: 1972 2113 2364 2842 3324 3478 3845 4230 4247 Size: 141 251 478 482 154 367 385 17 Cuts at: 4247 5699 5785 5797 6143 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 482 478 385 367 346 251 154 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5596 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2763 487 Size: 683 56 1537 3872 Fragments arranged by size: 3872 1537 683 56 BtsI GCAGTG_nn' Cuts at: 2697 3364 3664 4458 4478 2697 Size: 667 300 794 20 4367 Fragments arranged by size: 4367 794 667 300 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DraIII CAC_nnn'GTG Cuts at: 2442 2455 2442 Size: 13 6135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2192 5155 809 Size: 507 876 2963 1802 Fragments arranged by size: 2963 1802 876 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 3348 3595 Size: 54 1425 50 275 18 649 869 247 Cuts at: 3595 4484 6113 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 869 649 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2101 2345 2877 4078 1353 Size: 748 244 532 1201 3423 Fragments arranged by size: 3423 1201 748 532 244 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2639 3053 2639 Size: 414 5734 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2439 2841 2858 2907 2942 3111 Size: 393 473 334 402 17 49 35 169 Cuts at: 3111 3143 3341 3387 4211 4808 5595 5863 1239 Size: 32 198 46 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 473 402 393 334 268 198 169 49 46 35 32 17 EcoO109I rG'GnC_Cy Cuts at: 2140 3086 2140 Size: 946 5202 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2864 2864 Size: 6148 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 5295 1678 Size: 3617 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2375 2407 4789 Size: 32 135 32 461 32 310 32 2382 Cuts at: 4789 4821 4863 4895 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2382 461 310 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 2889 748 Size: 2141 4007 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KpnI G_GTAC'C Cuts at: 2622 3473 2622 Size: 851 5297 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2301 5262 5446 1305 Size: 996 2961 184 2007 Fragments arranged by size: 2961 2007 996 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 10 Size: 54 1425 343 649 3677 Fragments arranged by size: 3677 1425 649 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3231 4106 4465 4624 518 Size: 648 2065 875 359 159 2042 Fragments arranged by size: 2065 2042 875 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2375 3397 3564 4241 5389 5634 1606 Size: 498 271 1022 167 677 1148 245 2120 Fragments arranged by size: 2120 1148 1022 677 498 271 245 167 NaeI GCC'GGC Cuts at: 3355 3355 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5596 NdeI CA'TA_TG Cuts at: 387 3020 3205 387 Size: 2633 185 3330 Fragments arranged by size: 3330 2633 185 NgoMIV G'CCGG_C Cuts at: 3353 3353 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 3020 3020 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 2308 2518 3506 5051 1132 Size: 97 1079 210 988 1545 2229 Fragments arranged by size: 2229 1545 1079 988 210 97 PasI CC'CwG_GG Cuts at: 1348 2069 1348 Size: 721 5427 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2459 2491 4217 4249 5730 5762 2459 Size: 32 1726 32 1481 32 2845 Fragments arranged by size: 2845 1726 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 3086 2140 Size: 946 5202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2707 3500 830 Size: 1877 793 3478 Fragments arranged by size: 3478 1877 793 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2104 2375 2104 Size: 271 5877 SacI G_AGCT'C Cuts at: 2641 3055 2641 Size: 414 5734 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2877 2877 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2288 2703 3496 4631 5312 5503 826 Size: 219 1243 415 793 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 793 681 415 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4252 5153 5415 820 Size: 197 926 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 2015 1517 Size: 498 5650 StyI C'CwwG_G Cuts at: 513 1065 3587 513 Size: 552 2522 3074 Fragments arranged by size: 3074 2522 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4175 4192 4345 4530 4975 325 Size: 1255 2595 17 153 185 445 1498 Fragments arranged by size: 2595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4394 6106 96 Size: 274 80 33 51 1443 2417 1712 138 Fragments arranged by size: 2417 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2048 2080 6108 6140 2048 Size: 32 4028 32 2056 Fragments arranged by size: 4028 2056 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 2560 3484 2560 Size: 924 5224 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 2844 2871 4277 2844 Size: 27 1406 4715 Fragments arranged by size: 4715 1406 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI