Restriction map for
pFN21AB0417
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI NcoI NdeI
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 100 CGCG CG^CG
AcyI 86 GGCGCC G[GA]^CG[CT]C
AfaI 852 GTAC GT^AC
AlwNI 1138 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1346 GGGCCC GGGCC^C
AspI 1105 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 607 GGATCC G^GATCC
BanI 86 GGCGCC G^G[CT][GA]CC
BbeI 86 GGCGCC GGCGC^C
BclI 113 TGATCA T^GATCA
BcnI 1344 CCGGG CC^[GC]GG
BfaI 925 CTAG C^TAG
BsaHI 86 GGCGCC G[GA]^CG[CT]C
BsmAI 486 GAGAC GTCTC(1/5)
BsmBI 486 GAGACG CGTCTC(1/5)
BsmFI 865 GGGAC GGGAC(10/14)
BspMI 257 GCAGGT ACCTGC(4/8)
BsrDI 717 CATTGC GCAATG(2/0)
BstAPI 357 GCAACGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 769 TTCGAA TT^CGAA
BstUI 100 CGCG CG^CG
DraII 865 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1295 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 865 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 1225 GATATC GAT^ATC
EcoT22I 549 ATGCAT ATGCA^T
FbaI 113 TGATCA T^GATCA
HaeII 86 GGCGCC [GA]GCGC^[CT]
Hin1I 86 GGCGCC G[GA]^CG[CT]C
HincII 171 GTTAAC GT[CT]^[GA]AC
HindII 171 GTTAAC GT[CT]^[GA]AC
HpaI 171 GTTAAC GTT^AAC
KasI 86 GGCGCC G^GCGCC
MaeI 925 CTAG C^TAG
MaeII 299 ACGT A^CGT
MvnI 100 CGCG CG^CG
NaeI 83 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NciI 1344 CCGGG CC^[GC]GG
NgoMIV 83 GCCGGC G^CCGGC
NsiI 549 ATGCAT ATGCA^T
NspV 769 TTCGAA TT^CGAA
PflFI 1105 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 865 GGGACCC [GA]G^G[AT]CC[CT]
PstI 255 CTGCAG CTGCA^G
RsaI 852 GTAC GT^AC
ScaI 851 AGTACT AGT^ACT
SfoI 86 GGCGCC GGC^GCC
SfuI 769 TTCGAA TT^CGAA
SmlI 1019 CTCAAG C^T[CT][GA]AG
StuI 945 AGGCCT AGG^CCT
TaqI 770 TCGA T^CGA
Tsp45I 820 GTGAC ^GT[GC]AC
Tth111I 1105 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 770 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 6 AAATTT [GA]^AATT[CT]
766 AAATTC
AocI 870 CCTGAGG CC^T[ACGT]AGG
1135 CCTCAGG
ApoI 6 AAATTT [GA]^AATT[CT]
766 AAATTC
Asp700I 186 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
762 GAAGAAATTC
AspHI 331 GAGCTC G[AT]GC[AT]^C
671 GAGCTC
AvaI 862 CTCGGG C^[CT]CG[GA]G
941 CCCGAG
BalI 30 TGGCCA TGG^CCA
680 TGGCCA
BbsI 427 GAAGAC GAAGAC(2/6)
1303 GAAGAC
BpuAI 427 GAAGAC GAAGAC(2/6)
1303 GAAGAC
BseRI 150 GAGGAG GAGGAG(10/8)
208 GAGGAG
BsiHKAI 331 GAGCTC G[AT]GC[AT]^C
671 GAGCTC
BsoBI 862 CTCGGG C^[CT]CG[GA]G
941 CCCGAG
BsrFI 83 GCCGGC [GA]^CCGG[CT]
953 ACCGGC
BstXI 839 CCATTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1073 CCATGAGTTTGG
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
1135 CCTCAGG
CfoI 87 GCGC GCG^C
915 GCGC
Cfr10I 83 GCCGGC [GA]^CCGG[CT]
953 ACCGGC
DsaI 613 CCGTGG C^C[GA][CT]GG
984 CCACGG
EaeI 30 TGGCCA [CT]^GGCC[GA]
680 TGGCCA
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
1135 CCTCAGG
EcoNI 27 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
204 CCTGGAGGAGG
FauI 564 CCCGC CCCGC(4/6)
1323 GCGGG
HgaI 101 GCGTC GACGC(5/10)
391 GCGTC
HhaI 87 GCGC GCG^C
915 GCGC
HinP1I 87 GCGC G^CGC
915 GCGC
HindIII 476 AAGCTT A^AGCTT
1036 AAGCTT
MluNI 30 TGGCCA TGG^CCA
680 TGGCCA
MscI 30 TGGCCA TGG^CCA
680 TGGCCA
MseI 172 TTAA T^TAA
1387 TTAA
NspI 362 GCATGC [GA]CATG^[CT]
551 GCATGC
PleI 822 GACTC GAGTC(4/5)
1109 GAGTC
SacI 331 GAGCTC GAGCT^C
671 GAGCTC
SfcI 255 CTGCAG C^T[GA][CT]AG
1118 CTACAG
SphI 362 GCATGC GCATG^C
551 GCATGC
Tru9I 172 TTAA T^TAA
1387 TTAA
XmnI 186 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
762 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp509I TspRI XhoII