(Circular) (Six-base) MAPSORT of: pFN21AB0417.seq Check: 2744 from: 1 to: 6118 pFN21AB0417 6118 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 3303 4181 5565 5833 1632 Size: 704 967 878 1384 268 1917 Fragments arranged by size: 1917 1384 967 878 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 3148 3861 5433 3148 Size: 713 1572 3833 Fragments arranged by size: 3833 1572 713 ApaI G_GGCC'C Cuts at: 3355 3355 Size: 6118 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2011 2771 3427 3653 3805 5914 1493 Size: 518 760 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 760 656 518 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 2867 2946 3443 1870 Size: 73 924 79 497 4545 Fragments arranged by size: 4545 924 497 79 73 BamHI G'GATC_C Cuts at: 2612 3448 3815 2612 Size: 836 367 4915 Fragments arranged by size: 4915 836 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 3439 4894 618 Size: 325 217 627 304 1348 1455 1842 Fragments arranged by size: 1842 1455 1348 627 325 304 217 BanII G_rGCy'C Cuts at: 1391 2340 2680 3355 3437 1391 Size: 949 340 675 82 4072 Fragments arranged by size: 4072 949 675 340 82 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 6118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 3315 928 Size: 971 540 876 3731 Fragments arranged by size: 3731 971 876 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 2118 1548 Size: 570 5548 BfrBI ATG'CAT Cuts at: 2556 2556 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2166 2407 3355 4179 5335 1790 Size: 376 241 948 824 1156 2573 Fragments arranged by size: 2573 1156 948 824 376 241 BmgBI CAC'GTC Cuts at: 1318 2305 1318 Size: 987 5131 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3311 3849 4807 Size: 416 53 83 399 24 1894 538 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1894 1753 958 538 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2150 2231 2664 2693 3246 4778 1239 Size: 884 27 81 433 29 553 1532 2579 Fragments arranged by size: 2579 1532 884 553 433 81 29 27 Bpu10I CC'TnA_GC Cuts at: 2630 2630 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3009 4243 5108 5406 3009 Size: 1234 865 298 3721 Fragments arranged by size: 3721 1234 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 3925 4307 4942 Size: 53 83 186 936 559 1833 382 635 Cuts at: 4942 275 Size: 1451 Fragments arranged by size: 1833 1451 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2812 2842 2970 3000 1178 Size: 30 165 30 1409 30 128 30 4296 Fragments arranged by size: 4296 1409 165 128 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2170 2228 1186 Size: 984 58 5076 Fragments arranged by size: 5076 984 58 BseYI C'CCAG_C Cuts at: 5321 5817 5321 Size: 496 5622 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5545 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2340 2680 4179 4264 5335 2340 Size: 340 1499 85 1071 3123 Fragments arranged by size: 3123 1499 1071 340 85 BsmI GAATG_Cn' Cuts at: 3629 3722 3629 Size: 93 6025 BsmBI CGTCTCn'nnnn_ Cuts at: 2485 2485 Size: 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2340 2407 2680 3355 3437 4179 Size: 399 376 174 67 273 675 82 742 Cuts at: 4179 4264 5335 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 675 399 376 273 174 85 82 67 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 3473 844 Size: 1409 1220 3489 Fragments arranged by size: 3489 1409 1220 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 4613 4787 65 Size: 1183 1473 1892 174 1396 Fragments arranged by size: 1892 1473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2958 4762 4949 1700 Size: 110 278 870 1804 187 2869 Fragments arranged by size: 2869 1804 870 278 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4544 BstBI TT'CG_AA Cuts at: 2775 4922 2775 Size: 2147 3971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstXI CCAn_nnnn'nTGG Cuts at: 2851 3085 2851 Size: 234 5884 BstYI r'GATC_y Cuts at: 1972 2249 2565 2612 3261 3448 3815 4200 4217 Size: 277 316 47 649 187 367 385 17 Cuts at: 4217 5669 5755 5767 6113 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 649 385 367 346 316 277 187 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 3141 2876 Size: 265 5853 BtgI C'CryG_G Cuts at: 513 1065 2004 2618 2989 513 Size: 552 939 614 371 3642 Fragments arranged by size: 3642 939 614 552 371 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 3634 4428 4448 3634 Size: 794 20 5304 Fragments arranged by size: 5304 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DraIII CAC_nnn'GTG Cuts at: 3305 3305 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2685 3565 Size: 54 1425 50 275 18 205 650 880 Cuts at: 3565 4454 6083 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 880 650 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 2300 2455 4048 1353 Size: 702 245 155 1593 3423 Fragments arranged by size: 3423 1593 702 245 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2820 4705 5079 5225 387 Size: 1064 138 1231 1885 374 146 1280 Fragments arranged by size: 1885 1280 1231 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2338 2678 2338 Size: 340 5778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2150 2231 2336 2664 2693 3246 Size: 393 491 27 81 105 328 29 553 Cuts at: 3246 3303 4181 4778 5565 5833 1239 Size: 57 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 597 553 491 393 328 268 105 81 57 29 27 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2213 2036 Size: 177 5941 EcoO109I rG'GnC_Cy Cuts at: 2871 2871 Size: 6118 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 EcoRV GAT'ATC Cuts at: 3232 3232 Size: 6118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3059 3059 Size: 6118 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2095 5265 1678 Size: 417 3170 2531 Fragments arranged by size: 3170 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2241 2273 2487 Size: 32 135 32 538 32 99 32 214 Cuts at: 2487 2519 2818 2850 3164 3196 4759 4791 4833 Size: 32 299 32 314 32 1563 32 42 Cuts at: 4833 4865 1373 Size: 32 2626 Fragments arranged by size: 2626 1563 538 314 299 214 135 99 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2178 3462 3708 669 Size: 988 281 240 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 240 HindIII A'AGCT_T Cuts at: 748 2481 3041 748 Size: 1733 560 3825 Fragments arranged by size: 3825 1733 560 HpaI GTT'AAC Cuts at: 2178 3708 2178 Size: 1530 4588 KasI G'GCGC_C Cuts at: 2091 2091 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 5232 5416 1305 Size: 1563 2364 184 2007 Fragments arranged by size: 2364 2007 1563 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2037 2687 10 Size: 54 1425 343 205 650 3441 Fragments arranged by size: 3441 1425 650 343 205 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2551 2849 4076 4435 4594 518 Size: 648 1020 365 298 1227 359 159 2042 Fragments arranged by size: 2042 1227 1020 648 365 359 298 159 MspA1I CmG'CkG Cuts at: 1606 3534 4211 5359 5604 1606 Size: 1928 677 1148 245 2120 Fragments arranged by size: 2120 1928 1148 677 245 NaeI GCC'GGC Cuts at: 2090 2090 Size: 6118 NarI GG'CG_CC Cuts at: 2092 2092 Size: 6118 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NgoMIV G'CCGG_C Cuts at: 2088 2088 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NsiI A_TGCA'T Cuts at: 2558 2558 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 2371 2560 3476 5021 1132 Size: 97 1142 189 916 1545 2229 Fragments arranged by size: 2229 1545 1142 916 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PfoI T'CCnGG_A Cuts at: 2139 2139 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2871 2871 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 2658 3688 2658 Size: 1030 5088 PspOMI G'GGCC_C Cuts at: 3351 3351 Size: 6118 PstI C_TGCA'G Cuts at: 830 2264 3470 830 Size: 1434 1206 3478 Fragments arranged by size: 3478 1434 1206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 SacI G_AGCT'C Cuts at: 2340 2680 2340 Size: 340 5778 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SanDI GG'GwC_CC Cuts at: 2871 2871 Size: 6118 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 2858 4366 6078 2858 Size: 1508 1712 2898 Fragments arranged by size: 2898 1712 1508 SfcI C'TryA_G Cuts at: 826 1045 2260 3123 3466 4601 5282 5473 826 Size: 219 1215 863 343 1135 681 191 1471 Fragments arranged by size: 1471 1215 1135 863 681 343 219 191 SfoI GGC'GCC Cuts at: 2093 2093 Size: 6118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3024 4222 5123 5385 820 Size: 197 926 1081 1198 901 262 1553 Fragments arranged by size: 1553 1198 1081 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 2371 2560 3476 1132 Size: 1239 189 916 3774 Fragments arranged by size: 3774 1239 916 189 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2952 1517 Size: 1435 4683 StyI C'CwwG_G Cuts at: 513 1065 2004 2032 2146 2389 3557 513 Size: 552 939 28 114 243 1168 3074 Fragments arranged by size: 3074 1168 939 552 243 114 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 3293 4145 4162 4315 4500 4945 Size: 1255 815 898 852 17 153 185 445 Cuts at: 4945 325 Size: 1498 Fragments arranged by size: 1498 1255 898 852 815 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 4364 6076 Size: 274 80 33 51 1443 879 1508 1712 Cuts at: 6076 96 Size: 138 Fragments arranged by size: 1712 1508 1443 879 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2305 2337 2970 3002 6078 6110 2305 Size: 32 633 32 3076 32 2313 Fragments arranged by size: 3076 2313 633 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3113 1316 Size: 1797 4321 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 2195 2771 4247 2195 Size: 576 1476 4066 Fragments arranged by size: 4066 1476 576 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BstZ17I FseI FspAI NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI