Restriction map for
pFN21AB0417
(Linear) (Six-base) MAPSORT of: KIBB0417.seq Check: 2128 from: 1 to: 1413
KIBB0417.orf
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1298 1413
Size: 331 967 115
Fragments arranged by size:
967 331 115
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1413
Size: 1143 270
ApaI G_GGCC'C
Cuts at: 0 1350 1413
Size: 1350 63
ApoI r'AATT_y
Cuts at: 0 6 766 1413
Size: 6 760 647
Fragments arranged by size:
760 647 6
AvaI C'yCGr_G
Cuts at: 0 862 941 1413
Size: 862 79 472
Fragments arranged by size:
862 472 79
BamHI G'GATC_C
Cuts at: 0 607 1413
Size: 607 806
BanI G'GyrC_C
Cuts at: 0 86 1413
Size: 86 1327
BanII G_rGCy'C
Cuts at: 0 335 675 1350 1413
Size: 335 340 675 63
Fragments arranged by size:
675 340 335 63
BbeI G_GCGC'C
Cuts at: 0 90 1413
Size: 90 1323
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 1310 1413
Size: 434 876 103
Fragments arranged by size:
876 434 103
BclI T'GATC_A
Cuts at: 0 113 1413
Size: 113 1300
BfrBI ATG'CAT
Cuts at: 0 551 1413
Size: 551 862
Bme1580I G_kGCm'C
Cuts at: 0 161 402 1350 1413
Size: 161 241 948 63
Fragments arranged by size:
948 241 161 63
BmgBI CAC'GTC
Cuts at: 0 300 1413
Size: 300 1113
BmrI ACTGGGnnnn_n'
Cuts at: 0 1306 1413
Size: 1306 107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 145 226 659 688 1241 1413
Size: 118 27 81 433 29 553 172
Fragments arranged by size:
553 433 172 118 81 29 27
Bpu10I CC'TnA_GC
Cuts at: 0 625 1413
Size: 625 788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1413
Size: 1004 409
BsaHI Gr'CG_yC
Cuts at: 0 87 1413
Size: 87 1326
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 807 837 965 995 1413
Size: 807 30 128 30 418
Fragments arranged by size:
807 418 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 165 223 1413
Size: 165 58 1190
Fragments arranged by size:
1190 165 58
BsiHKAI G_wGCw'C
Cuts at: 0 335 675 1413
Size: 335 340 738
Fragments arranged by size:
738 340 335
BsmBI CGTCTCn'nnnn_
Cuts at: 0 480 1413
Size: 480 933
Bsp1286I G_dGCh'C
Cuts at: 0 161 335 402 675 1350 1413
Size: 161 174 67 273 675 63
Fragments arranged by size:
675 273 174 161 67 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 1413
Size: 248 1165
BsrDI GCAATG_nn'
Cuts at: 0 716 1413
Size: 716 697
BsrFI r'CCGG_y
Cuts at: 0 83 953 1413
Size: 83 870 460
Fragments arranged by size:
870 460 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 1413
Size: 363 1050
BstBI TT'CG_AA
Cuts at: 0 770 1413
Size: 770 643
BstXI CCAn_nnnn'nTGG
Cuts at: 0 846 1080 1413
Size: 846 234 333
Fragments arranged by size:
846 333 234
BstYI r'GATC_y
Cuts at: 0 244 560 607 1256 1413
Size: 244 316 47 649 157
Fragments arranged by size:
649 316 244 157 47
Bsu36I CC'TnA_GG
Cuts at: 0 871 1136 1413
Size: 871 265 277
Fragments arranged by size:
871 277 265
BtgI C'CryG_G
Cuts at: 0 613 984 1413
Size: 613 371 429
Fragments arranged by size:
613 429 371
DraIII CAC_nnn'GTG
Cuts at: 0 1300 1413
Size: 1300 113
EaeI y'GGCC_r
Cuts at: 0 30 680 1413
Size: 30 650 733
Fragments arranged by size:
733 650 30
EarI CTCTTCn'nnn_
Cuts at: 0 50 295 450 1413
Size: 50 245 155 963
Fragments arranged by size:
963 245 155 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 815 1413
Size: 815 598
EcoICRI GAG'CTC
Cuts at: 0 333 673 1413
Size: 333 340 740
Fragments arranged by size:
740 340 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 145 226 331 659 688 1241 1298
Size: 118 27 81 105 328 29 553 57
Cuts at: 1298 1413
Size: 115
Fragments arranged by size:
553 328 118 115 105 81 57 29
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 208 1413
Size: 31 177 1205
Fragments arranged by size:
1205 177 31
EcoO109I rG'GnC_Cy
Cuts at: 0 866 1413
Size: 866 547
EcoRV GAT'ATC
Cuts at: 0 1227 1413
Size: 1227 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1054 1413
Size: 1054 359
HaeII r_GCGC'y
Cuts at: 0 90 1413
Size: 90 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 236 268 482 514 813 845
Size: 105 32 99 32 214 32 299 32
Cuts at: 845 1159 1191 1413
Size: 314 32 222
Fragments arranged by size:
314 299 222 214 105 99 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 173 1413
Size: 173 1240
HindIII A'AGCT_T
Cuts at: 0 476 1036 1413
Size: 476 560 377
Fragments arranged by size:
560 476 377
HpaI GTT'AAC
Cuts at: 0 173 1413
Size: 173 1240
KasI G'GCGC_C
Cuts at: 0 86 1413
Size: 86 1327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1413
Size: 863 550
MscI TGG'CCA
Cuts at: 0 32 682 1413
Size: 32 650 731
Fragments arranged by size:
731 650 32
MslI CAynn'nnrTG
Cuts at: 0 181 546 844 1413
Size: 181 365 298 569
Fragments arranged by size:
569 365 298 181
NaeI GCC'GGC
Cuts at: 0 85 1413
Size: 85 1328
NarI GG'CG_CC
Cuts at: 0 87 1413
Size: 87 1326
NgoMIV G'CCGG_C
Cuts at: 0 83 1413
Size: 83 1330
NsiI A_TGCA'T
Cuts at: 0 553 1413
Size: 553 860
NspI r_CATG'y
Cuts at: 0 366 555 1413
Size: 366 189 858
Fragments arranged by size:
858 366 189
PfoI T'CCnGG_A
Cuts at: 0 134 1413
Size: 134 1279
PpuMI rG'GwC_Cy
Cuts at: 0 866 1413
Size: 866 547
PsiI TTA'TAA
Cuts at: 0 653 1413
Size: 653 760
PspOMI G'GGCC_C
Cuts at: 0 1346 1413
Size: 1346 67
PstI C_TGCA'G
Cuts at: 0 259 1413
Size: 259 1154
SacI G_AGCT'C
Cuts at: 0 335 675 1413
Size: 335 340 738
Fragments arranged by size:
738 340 335
SanDI GG'GwC_CC
Cuts at: 0 866 1413
Size: 866 547
ScaI AGT'ACT
Cuts at: 0 853 1413
Size: 853 560
SfcI C'TryA_G
Cuts at: 0 255 1118 1413
Size: 255 863 295
Fragments arranged by size:
863 295 255
SfoI GGC'GCC
Cuts at: 0 88 1413
Size: 88 1325
SmlI C'TyrA_G
Cuts at: 0 1019 1413
Size: 1019 394
SphI G_CATG'C
Cuts at: 0 366 555 1413
Size: 366 189 858
Fragments arranged by size:
858 366 189
StuI AGG'CCT
Cuts at: 0 947 1413
Size: 947 466
StyI C'CwwG_G
Cuts at: 0 27 141 384 1413
Size: 27 114 243 1029
Fragments arranged by size:
1029 243 114 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 390 1288 1413
Size: 390 898 125
Fragments arranged by size:
898 390 125
TatI w'GTAC_w
Cuts at: 0 851 1413
Size: 851 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 965 997 1413
Size: 300 32 633 32 416
Fragments arranged by size:
633 416 300 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1108 1413
Size: 1108 305
XmnI GAAnn'nnTTC
Cuts at: 0 190 766 1413
Size: 190 576 647
Fragments arranged by size:
647 576 190
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BclI BfrBI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I
BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI
KasI MmeI MscI MslI NaeI NarI NgoMIV NsiI
NspI PfoI PpuMI PsiI PspOMI PstI SacI SanDI
ScaI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BglI BglII
BlpI BmtI BplI BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoRI FseI FspI FspAI KpnI
MfeI MluI MspA1I NcoI NdeI NheI NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI PvuII RsrII SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI