(Circular) (Six-base) MAPSORT of: pFN21AE0644.seq Check: 5645 from: 1 to: 5998 pFN21AE0644 5998 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2381 3341 1937 Size: 172 272 960 4594 Fragments arranged by size: 4594 960 272 172 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4061 5445 5713 1632 Size: 2429 1384 268 1917 Fragments arranged by size: 2429 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 2105 4727 2105 Size: 2622 3376 AleI CACnn'nnGTG Cuts at: 1166 2159 2542 1166 Size: 993 383 4622 Fragments arranged by size: 4622 993 383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2246 2381 1184 Size: 1062 135 4801 Fragments arranged by size: 4801 1062 135 AlwNI CAG_nnn'CTG Cuts at: 3741 5313 3741 Size: 1572 4426 ApaLI G'TGCA_C Cuts at: 2630 4055 5211 2630 Size: 1425 1156 3417 Fragments arranged by size: 3417 1425 1156 ApoI r'AATT_y Cuts at: 1493 3094 3274 3307 3533 3685 5794 1493 Size: 1601 180 33 226 152 2109 1697 Fragments arranged by size: 2109 1697 1601 226 180 152 33 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3323 1870 Size: 73 1380 4545 Fragments arranged by size: 4545 1380 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2365 2433 2863 3319 4774 Size: 325 217 627 578 68 430 456 1455 Cuts at: 4774 618 Size: 1842 Fragments arranged by size: 1842 1455 627 578 456 430 325 217 68 BanII G_rGCy'C Cuts at: 1391 3317 1391 Size: 1926 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5027 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2055 4189 4223 1903 Size: 34 50 34 34 2134 34 3678 Fragments arranged by size: 3678 2134 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 1548 Size: 5998 BfrBI ATG'CAT Cuts at: 3002 3002 Size: 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2141 4609 Size: 107 122 71 631 605 337 132 2468 Cuts at: 4609 136 Size: 1525 Fragments arranged by size: 2468 1525 631 605 337 132 122 107 71 BglII A'GATC_T Cuts at: 2640 5993 2640 Size: 3353 2645 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2634 2866 4059 5215 1790 Size: 844 232 1193 1156 2573 Fragments arranged by size: 2573 1193 1156 844 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3729 4687 442 Size: 416 53 83 399 24 2312 958 1753 Fragments arranged by size: 2312 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 3060 4658 1239 Size: 1061 760 1598 2579 Fragments arranged by size: 2579 1598 1061 760 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2582 4123 4988 5286 2582 Size: 1541 865 298 3294 Fragments arranged by size: 3294 1541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2209 2551 3789 493 Size: 711 1005 342 1238 2702 Fragments arranged by size: 2702 1238 1005 711 342 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3058 3088 1178 Size: 30 165 30 1655 30 4088 Fragments arranged by size: 4088 1655 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3233 1186 Size: 2047 3951 BseYI C'CCAG_C Cuts at: 2338 2859 5201 5697 2338 Size: 521 2342 496 2639 Fragments arranged by size: 2639 2342 521 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 3448 4209 5237 1540 Size: 275 186 101 1346 761 1028 2301 Fragments arranged by size: 2301 1346 1028 761 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2154 2166 2634 4059 4144 5215 2154 Size: 12 468 1425 85 1071 2937 Fragments arranged by size: 2937 1425 1071 468 85 12 BsmI GAATG_Cn' Cuts at: 2298 3173 3509 3602 2298 Size: 875 336 93 4694 Fragments arranged by size: 4694 875 336 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2166 2634 2866 3317 4059 4144 Size: 399 364 12 468 232 451 742 85 Cuts at: 4144 5215 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 468 451 399 364 232 85 12 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2286 2423 3353 844 Size: 1442 137 930 3489 Fragments arranged by size: 3489 1442 930 137 BsrBI CCG'CTC Cuts at: 1394 1823 2104 3885 1394 Size: 429 281 1781 3507 Fragments arranged by size: 3507 1781 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 2197 2418 4493 4667 65 Size: 1183 949 221 2075 174 1396 Fragments arranged by size: 2075 1396 1183 949 221 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 2156 4642 4829 1700 Size: 110 281 65 2486 187 2869 Fragments arranged by size: 2869 2486 281 187 110 65 BsrGI T'GTAC_A Cuts at: 96 2703 96 Size: 2607 3391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 2147 4058 5070 2147 Size: 1911 1012 3075 Fragments arranged by size: 3075 1911 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5998 BstBI TT'CG_AA Cuts at: 2764 4802 2764 Size: 2038 3960 BstEII G'GTnAC_C Cuts at: 1184 2212 1184 Size: 1028 4970 BstYI r'GATC_y Cuts at: 1972 2640 3328 3695 4080 4097 5549 5635 5647 Size: 668 688 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 688 668 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5446 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2065 487 Size: 683 56 839 4420 Fragments arranged by size: 4420 839 683 56 BtsI GCAGTG_nn' Cuts at: 3514 4308 4328 3514 Size: 794 20 5184 Fragments arranged by size: 5184 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 2458 3302 2458 Size: 844 5154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3445 4334 5963 Size: 54 1425 50 275 18 1615 889 1629 Cuts at: 5963 8 Size: 43 Fragments arranged by size: 1629 1615 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 2675 3928 1353 Size: 1254 68 1253 3423 Fragments arranged by size: 3423 1254 1253 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 3060 4061 4658 5445 5713 1239 Size: 393 668 760 1001 597 787 268 1524 Fragments arranged by size: 1524 1001 787 760 668 597 393 268 EcoRI G'AATT_C Cuts at: 3274 3307 3685 3274 Size: 33 378 5587 Fragments arranged by size: 5587 378 33 FspI TGC'GCA Cuts at: 2234 4504 2234 Size: 2270 3728 FspAI rTGC'GCAy Cuts at: 2234 2234 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2015 2183 2270 5145 1678 Size: 337 168 87 2875 2531 Fragments arranged by size: 2875 2531 337 168 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 4639 4671 4713 Size: 32 135 32 519 32 2516 32 42 Cuts at: 4713 4745 1373 Size: 32 2626 Fragments arranged by size: 2626 2516 519 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2352 2997 5112 5296 1305 Size: 1047 645 2115 184 2007 Fragments arranged by size: 2115 2007 1047 645 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4176 Fragments arranged by size: 4176 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2423 2542 2825 3956 4315 4474 Size: 648 993 264 119 283 1131 359 159 Cuts at: 4474 518 Size: 2042 Fragments arranged by size: 2042 1131 993 648 359 283 264 159 119 MspA1I CmG'CkG Cuts at: 1606 2387 3414 4091 5239 5484 1606 Size: 781 1027 677 1148 245 2120 Fragments arranged by size: 2120 1148 1027 781 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5446 NdeI CA'TA_TG Cuts at: 387 2883 387 Size: 2496 3502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NsiI A_TGCA'T Cuts at: 3004 3004 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3299 3356 4901 1132 Size: 97 2070 57 1545 2229 Fragments arranged by size: 2229 2070 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PmlI CAC'GTG Cuts at: 2551 2551 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PstI C_TGCA'G Cuts at: 830 3043 3350 830 Size: 2213 307 3478 Fragments arranged by size: 3478 2213 307 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 3039 3346 4481 5162 5353 826 Size: 219 1994 307 1135 681 191 1471 Fragments arranged by size: 1994 1471 1135 681 307 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 2156 1700 Size: 456 5542 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2561 4102 5003 5265 820 Size: 197 926 618 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 618 262 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2262 3437 513 Size: 552 1197 1175 3074 Fragments arranged by size: 3074 1197 1175 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 4025 4042 4195 4380 4825 325 Size: 1255 974 1471 17 153 185 445 1498 Fragments arranged by size: 1498 1471 1255 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2703 4244 5956 Size: 274 80 33 51 1443 726 1541 1712 Cuts at: 5956 96 Size: 138 Fragments arranged by size: 1712 1541 1443 726 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5958 5990 5958 Size: 32 5966 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI