(Circular) (Six-base) MAPSORT of: pFN21AE0785.seq Check: 6603 from: 1 to: 5764 pFN21AE0785 5764 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2098 2623 2098 Size: 525 5239 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 2871 3047 3085 2871 Size: 176 38 5550 Fragments arranged by size: 5550 176 38 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2266 2684 2890 3827 5211 5479 1632 Size: 634 418 206 937 1384 268 1917 Fragments arranged by size: 1917 1384 937 634 418 268 206 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2208 4663 5640 1203 Size: 1005 2455 977 1327 Fragments arranged by size: 2455 1327 1005 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2622 1166 Size: 1456 4308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2480 1184 Size: 1296 4468 AlwNI CAG_nnn'CTG Cuts at: 2485 2788 3507 5079 2485 Size: 303 719 1572 3170 Fragments arranged by size: 3170 1572 719 303 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2558 3073 3299 3451 5560 1493 Size: 1065 515 226 152 2109 1697 Fragments arranged by size: 2109 1697 1065 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2323 2537 2769 3089 1870 Size: 73 380 214 232 320 4545 Fragments arranged by size: 4545 380 320 232 214 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3039 3072 3039 Size: 33 5731 BamHI G'GATC_C Cuts at: 3094 3461 3094 Size: 367 5397 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2533 2859 2871 2899 3047 Size: 325 217 627 746 326 12 28 148 Cuts at: 3047 3085 4540 618 Size: 38 1455 1842 Fragments arranged by size: 1842 1455 746 627 326 325 217 148 38 28 12 BanII G_rGCy'C Cuts at: 1391 2770 3083 1391 Size: 1379 313 4072 Fragments arranged by size: 4072 1379 313 BbeI G_GCGC'C Cuts at: 2537 2537 Size: 5764 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4793 BbvCI CC'TCA_GC Cuts at: 3039 3039 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2657 2691 3955 3989 1903 Size: 34 50 34 636 34 1264 34 3678 Fragments arranged by size: 3678 1264 636 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2594 4375 136 Size: 107 122 71 631 605 922 1781 1525 Fragments arranged by size: 1781 1525 922 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 2423 3181 2423 Size: 758 5006 Bme1580I G_kGCm'C Cuts at: 1790 2904 3825 4981 1790 Size: 1114 921 1156 2573 Fragments arranged by size: 2573 1156 1114 921 BmgBI CAC'GTC Cuts at: 1318 2209 1318 Size: 891 4873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 4424 1239 Size: 851 2334 2579 Fragments arranged by size: 2579 2334 851 Bpu10I CC'TnA_GC Cuts at: 3039 3039 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3889 4754 5052 2636 Size: 1253 865 298 3348 Fragments arranged by size: 3348 1253 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2534 3571 3953 4588 Size: 53 83 186 936 1001 1037 382 635 Cuts at: 4588 275 Size: 1451 Fragments arranged by size: 1451 1037 1001 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2896 4197 4595 4869 5016 5427 2896 Size: 1301 398 274 147 411 3233 Fragments arranged by size: 3233 1301 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2619 2649 1178 Size: 30 165 30 1216 30 4293 Fragments arranged by size: 4293 1216 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5764 BseYI C'CCAG_C Cuts at: 2148 2171 2485 2519 2806 3032 4967 5463 2148 Size: 23 314 34 287 226 1935 496 2449 Fragments arranged by size: 2449 1935 496 314 287 226 34 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2342 1163 Size: 573 390 216 4585 Fragments arranged by size: 4585 573 390 216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2378 3214 3975 5003 1540 Size: 275 186 377 836 761 1028 2301 Fragments arranged by size: 2301 1028 836 761 377 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 3825 3910 4981 2220 Size: 1605 85 1071 3003 Fragments arranged by size: 3003 1605 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2770 2904 3083 3825 3910 4981 Size: 399 430 550 134 179 742 85 1071 Cuts at: 4981 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 430 399 179 134 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 2623 2857 3035 3119 844 Size: 1254 525 234 178 84 3489 Fragments arranged by size: 3489 1254 525 234 178 84 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2530 4408 4595 1700 Size: 110 720 1878 187 2869 Fragments arranged by size: 2869 1878 720 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 2334 2460 1925 Size: 409 126 5229 Fragments arranged by size: 5229 409 126 BssSI C'ACGA_G Cuts at: 2393 3824 4836 2393 Size: 1431 1012 3321 Fragments arranged by size: 3321 1431 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2899 794 Size: 2105 3659 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstXI CCAn_nnnn'nTGG Cuts at: 2836 2836 Size: 5764 BstYI r'GATC_y Cuts at: 1972 3094 3461 3846 3863 5315 5401 5413 5759 Size: 1122 367 385 17 1452 86 12 346 Cuts at: 5759 1972 Size: 1977 Fragments arranged by size: 1977 1452 1122 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2296 2583 3051 513 Size: 552 1231 287 468 3226 Fragments arranged by size: 3226 1231 552 468 287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2332 487 Size: 683 56 1106 3919 Fragments arranged by size: 3919 1106 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2832 3002 2832 Size: 170 5594 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2375 2586 2941 Size: 54 1425 50 275 18 545 211 355 Cuts at: 2941 2961 3211 4100 5729 8 Size: 20 250 889 1629 43 Fragments arranged by size: 1629 1425 889 545 355 275 250 211 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2375 3211 1537 Size: 275 563 836 4090 Fragments arranged by size: 4090 836 563 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3694 1353 Size: 2341 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2255 4351 4725 4871 387 Size: 1064 138 498 168 2096 374 146 1280 Fragments arranged by size: 2096 1280 1064 498 374 168 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2266 2684 2890 3827 4424 5211 Size: 393 458 176 418 206 937 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 937 787 597 458 418 393 268 206 176 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2105 Size: 5764 EcoO109I rG'GnC_Cy Cuts at: 2489 2596 2693 2846 2489 Size: 107 97 153 5407 Fragments arranged by size: 5407 153 107 97 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 2069 2537 4911 1678 Size: 391 468 2374 2531 Fragments arranged by size: 2531 2374 468 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2032 2064 2960 2992 4405 Size: 32 135 32 460 32 896 32 1413 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1413 896 460 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2431 3108 3354 669 Size: 988 281 493 677 246 3079 Fragments arranged by size: 3079 988 677 493 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KasI G'GCGC_C Cuts at: 2533 2533 Size: 5764 KpnI G_GTAC'C Cuts at: 2875 3051 3089 2875 Size: 176 38 5550 Fragments arranged by size: 5550 176 38 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2588 2963 10 Size: 54 1425 343 756 375 2811 Fragments arranged by size: 2811 1425 756 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2622 3722 4081 4240 518 Size: 648 1456 1100 359 159 2042 Fragments arranged by size: 2042 1456 1100 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2233 2286 2298 2371 2389 2485 2579 Size: 565 62 53 12 73 18 96 94 Cuts at: 2579 2910 3180 3857 5005 5250 1606 Size: 331 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 331 270 245 96 94 73 62 53 18 12 NarI GG'CG_CC Cuts at: 2534 2534 Size: 5764 NcoI C'CATG_G Cuts at: 513 1065 2583 3051 513 Size: 552 1518 468 3226 Fragments arranged by size: 3226 1518 552 468 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 4627 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 2693 2846 2489 Size: 204 153 5407 Fragments arranged by size: 5407 204 153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspXI vC'TCGA_Gb Cuts at: 2323 2323 Size: 5764 PstI C_TGCA'G Cuts at: 830 2481 2784 2994 3116 830 Size: 1651 303 210 122 3478 Fragments arranged by size: 3478 1651 303 210 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2171 2171 Size: 5764 RsrII CG'GwC_CG Cuts at: 2528 2528 Size: 5764 SacII CC_GC'GG Cuts at: 2299 2299 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SanDI GG'GwC_CC Cuts at: 2489 2693 2489 Size: 204 5560 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2874 2874 Size: 5764 SfcI C'TryA_G Cuts at: 826 1045 2317 2477 2780 2990 3112 4247 4928 Size: 219 1272 160 303 210 122 1135 681 Cuts at: 4928 5119 826 Size: 191 1471 Fragments arranged by size: 1471 1272 1135 681 303 219 210 191 160 122 SfiI GGCCn_nnn'nGGCC Cuts at: 2594 2594 Size: 5764 SfoI GGC'GCC Cuts at: 2535 2535 Size: 5764 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 2539 3091 1872 Size: 667 552 4545 Fragments arranged by size: 4545 667 552 SmlI C'TyrA_G Cuts at: 820 1017 1943 2323 2651 3868 4769 5031 820 Size: 197 926 380 328 1217 901 262 1553 Fragments arranged by size: 1553 1217 926 901 380 328 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SrfI GCCC'GGGC Cuts at: 2539 2539 Size: 5764 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 2583 2761 2964 3051 3203 513 Size: 552 1518 178 203 87 152 3074 Fragments arranged by size: 3074 1518 552 203 178 152 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 3791 3808 3961 4146 4591 325 Size: 1255 560 1651 17 153 185 445 1498 Fragments arranged by size: 1651 1498 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2619 2651 5724 5756 2619 Size: 32 3073 32 2627 Fragments arranged by size: 3073 2627 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2145 2596 2145 Size: 451 5313 XhoI C'TCGA_G Cuts at: 1943 2323 1943 Size: 380 5384 XmaI C'CCGG_G Cuts at: 1870 2537 3089 1870 Size: 667 552 4545 Fragments arranged by size: 4545 667 552 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI SacI SapI SwaI