Restriction map for
pFN21AB5895
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaI BsiWI
BsmI Bsp1407I BspDI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MluNI MscI MslI MunI
NarI NdeI NheI NotI NruI
NspI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PstI PvuII RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 204 TCCGGA T^CCGGA
AcsI 1154 AAATTC [GA]^AATT[CT]
AcyI 1095 GACGCC G[GA]^CG[CT]C
AfaI 498 GTAC GT^AC
AlwNI 928 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1154 AAATTC [GA]^AATT[CT]
Asp700I 915 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1291 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 671 CCCGGG C^[CT]CG[GA]G
BbrPI 514 CACGTG CAC^GTG
BsaAI 514 CACGTG [CT]AC^GT[GA]
BsaHI 1095 GACGCC G[GA]^CG[CT]C
BseAI 204 TCCGGA T^CCGGA
BseRI 234 CTCCTC GAGGAG(10/8)
BsiEI 841 CGATCG CG[GA][CT]^CG
BsmBI 1093 GAGACG CGTCTC(1/5)
BsoBI 671 CCCGGG C^[CT]CG[GA]G
BspEI 204 TCCGGA T^CCGGA
BspHI 1241 TCATGA T^CATGA
BssSI 835 CACGAG CACGAG(-5/-1)
BstBI 191 TTCGAA TT^CGAA
EaeI 311 TGGCCG [CT]^GGCC[GA]
EcoRV 1225 GATATC GAT^ATC
EcoT22I 42 ATGCAT ATGCA^T
HgaI 1095 GACGC GACGC(5/10)
Hin1I 1095 GACGCC G[GA]^CG[CT]C
MroI 204 TCCGGA T^CCGGA
MseI 1066 TTAA T^TAA
NcoI 1312 CCATGG C^CATGG
NsiI 42 ATGCAT ATGCA^T
NspV 191 TTCGAA TT^CGAA
PflFI 1291 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1280 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 514 CACGTG CAC^GTG
PmlI 514 CACGTG CAC^GTG
PpuMI 588 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 4 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 841 CGATCG CGAT^CG
RcaI 1241 TCATGA T^CATGA
RsaI 498 GTAC GT^AC
SfcI 777 CTACAG C^T[GA][CT]AG
SfuI 191 TTCGAA TT^CGAA
SmaI 671 CCCGGG CCC^GGG
SmlI 107 CTCAAG C^T[CT][GA]AG
StuI 1338 AGGCCT AGG^CCT
Tru9I 1066 TTAA T^TAA
Tth111I 1291 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1280 CCAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
XmnI 915 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 509 GTGCAC G^TGCAC
847 GTGCAC
ApaLI 509 GTGCAC G^TGCAC
847 GTGCAC
AvaII 589 GGACC G^G[AT]CC
1296 GGTCC
BbsI 144 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BclI 1238 TGATCA T^GATCA
1244 TGATCA
BcnI 671 CCCGG CC^[GC]GG
672 CCGGG
BglI 413 GCCTTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1331 GCCACTCAGGC
BpmI 629 CTCCAG CTGGAG(16/14)
451 CTGGAG
BpuAI 144 GAAGAC GAAGAC(2/6)
1350 GAAGAC
BsaWI 204 TCCGGA [AT]^CCGG[AT]
443 TCCGGT
BsgI 871 CTGCAC GTGCAG(16/14)
1268 GTGCAG
BsmAI 1093 GAGAC GTCTC(1/5)
324 GTCTC
BstYI 296 GGATCT [GA]^GATC[CT]
1070 AGATCC
CfoI 316 GCGC GCG^C
1329 GCGC
DraII 588 GGGACCT [GA]G^G[ACGT]CC[CT]
929 AGGGCCT
EcoO109I 588 GGGACCT [GA]G^G[ACGT]CC[CT]
929 AGGGCCT
EcoT14I 66 CCTTGG C^C[AT][AT]GG
1312 CCATGG
FauI 410 CCCGC CCCGC(4/6)
586 GCGGG
FbaI 1238 TGATCA T^GATCA
1244 TGATCA
HhaI 316 GCGC GCG^C
1329 GCGC
HinP1I 316 GCGC G^CGC
1329 GCGC
KspI 584 CCGCGG CCGC^GG
994 CCGCGG
MflI 296 GGATCT [GA]^GATC[CT]
1070 AGATCC
NciI 671 CCCGG CC^[GC]GG
672 CCGGG
PleI 862 GACTC GAGTC(4/5)
1361 GAGTC
SacI 104 GAGCTC GAGCT^C
772 GAGCTC
SacII 584 CCGCGG CCGC^GG
994 CCGCGG
StyI 66 CCTTGG C^C[AT][AT]GG
1312 CCATGG
Tsp45I 908 GTGAC ^GT[GC]AC
1122 GTCAC
XhoII 296 GGATCT [GA]^GATC[CT]
1070 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AspHI BanII BbvI BcgI BfaI
BmyI BsaBI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I Cfr10I Cfr13I DdeI DpnII
DsaI EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV SapI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp509I TspRI TthHB8I