(Circular) (Six-base) MAPSORT of: pFN21AB5895.seq Check: 5236 from: 1 to: 6202 pFN21AB5895 6202 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 3523 3523 Size: 6202 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2911 3374 4265 5649 5917 1632 Size: 713 566 463 891 1384 268 1917 Fragments arranged by size: 1917 1384 891 713 566 463 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 2214 2518 2520 5101 6078 1203 Size: 1011 304 2 2581 977 1327 Fragments arranged by size: 2581 1327 1011 977 304 2 AgeI A'CCGG_T Cuts at: 5033 5033 Size: 6202 AhdI GACnn_n'nnGTC Cuts at: 4931 4931 Size: 6202 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AlwNI CAG_nnn'CTG Cuts at: 2938 3945 5517 2938 Size: 1007 1572 3623 Fragments arranged by size: 3623 1572 1007 ApaLI G'TGCA_C Cuts at: 2514 2852 4259 5415 2514 Size: 338 1407 1156 3301 Fragments arranged by size: 3301 1407 1156 338 ApoI r'AATT_y Cuts at: 1493 3159 3511 3737 3889 5998 1493 Size: 1666 352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1666 352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 2676 3527 1870 Size: 73 733 851 4545 Fragments arranged by size: 4545 851 733 73 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3523 4978 618 Size: 325 217 627 1736 1455 1842 Fragments arranged by size: 1842 1736 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2113 2750 2781 3040 3521 1391 Size: 722 637 31 259 481 4072 Fragments arranged by size: 4072 722 637 481 259 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 3362 928 Size: 971 257 1206 3768 Fragments arranged by size: 3768 1206 971 257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2416 2450 2459 2493 2527 2561 4393 Size: 34 479 34 9 34 34 34 1832 Cuts at: 4393 4427 1903 Size: 34 3678 Fragments arranged by size: 3678 1832 479 34 34 34 34 34 34 9 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 3243 3249 1548 Size: 1695 6 4501 Fragments arranged by size: 4501 1695 6 BfrBI ATG'CAT Cuts at: 2049 2049 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2424 3342 4813 Size: 107 122 71 631 605 752 918 1471 Cuts at: 4813 136 Size: 1525 Fragments arranged by size: 1525 1471 918 752 631 605 122 107 71 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 3619 3619 Size: 6202 Bme1580I G_kGCm'C Cuts at: 1790 2071 2518 2617 2856 4263 5419 1790 Size: 281 447 99 239 1407 1156 2573 Fragments arranged by size: 2573 1407 1156 447 281 239 99 BmgBI CAC'GTC Cuts at: 1318 2986 1318 Size: 1668 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3034 3933 4891 Size: 416 53 83 399 24 1617 899 958 Cuts at: 4891 442 Size: 1753 Fragments arranged by size: 1753 1617 958 899 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2619 4862 1239 Size: 1238 142 2243 2579 Fragments arranged by size: 2579 2243 1238 142 Bpu10I CC'TnA_GC Cuts at: 2693 2945 2693 Size: 252 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2097 4327 5192 5490 2097 Size: 2230 865 298 2809 Fragments arranged by size: 2809 2230 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4865 882 Size: 617 3366 2219 Fragments arranged by size: 3366 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3993 493 Size: 711 1317 1472 2702 Fragments arranged by size: 2702 1472 1317 711 BsaBI GATnn'nnATC Cuts at: 2198 2261 3248 6196 2198 Size: 63 987 2948 2204 Fragments arranged by size: 2948 2204 987 63 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3101 4009 4391 5026 Size: 53 83 186 936 1568 908 382 635 Cuts at: 5026 275 Size: 1451 Fragments arranged by size: 1568 1451 936 908 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2209 2448 4635 5033 5307 5454 5865 2209 Size: 239 2187 398 274 147 411 2546 Fragments arranged by size: 2546 2187 411 398 274 239 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 1178 Size: 30 165 30 1329 30 4618 Fragments arranged by size: 4618 1329 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 1186 Size: 1044 5158 BseYI C'CCAG_C Cuts at: 2340 2618 2796 2974 3384 5405 5901 2340 Size: 278 178 178 410 2021 496 2641 Fragments arranged by size: 2641 2021 496 410 278 178 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2861 3294 1163 Size: 573 1125 433 4071 Fragments arranged by size: 4071 1125 573 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2849 3652 4413 5441 1540 Size: 275 186 848 803 761 1028 2301 Fragments arranged by size: 2301 1028 848 803 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2113 2518 2685 2781 2856 3326 4263 4348 5419 Size: 405 167 96 75 470 937 85 1071 Cuts at: 5419 2113 Size: 2896 Fragments arranged by size: 2896 1071 937 470 405 167 96 85 75 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 BsmBI CGTCTCn'nnnn_ Cuts at: 3092 3092 Size: 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2113 2518 2617 2685 2750 2781 Size: 399 281 42 405 99 68 65 31 Cuts at: 2781 2856 3040 3326 3521 4263 4348 5419 1391 Size: 75 184 286 195 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 286 281 195 184 99 85 75 68 65 42 31 BspEI T'CCGG_A Cuts at: 2209 5865 2209 Size: 3656 2546 BspHI T'CATG_A Cuts at: 3246 4091 5821 3246 Size: 845 1730 3627 Fragments arranged by size: 3627 1730 845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3557 844 Size: 2713 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4089 1394 Size: 429 2266 3507 Fragments arranged by size: 3507 2266 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4697 4871 65 Size: 1183 3449 174 1396 Fragments arranged by size: 3449 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2335 2534 2882 4846 5033 1700 Size: 110 525 199 348 1964 187 2869 Fragments arranged by size: 2869 1964 525 348 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 2840 4262 5274 2840 Size: 1422 1012 3768 Fragments arranged by size: 3768 1422 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6202 BstBI TT'CG_AA Cuts at: 2197 5006 2197 Size: 2809 3393 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6202 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2653 3172 2038 Size: 615 519 5068 Fragments arranged by size: 5068 615 519 BstYI r'GATC_y Cuts at: 1972 2301 3075 3532 3899 4284 4301 5753 5839 Size: 329 774 457 367 385 17 1452 86 Cuts at: 5839 5851 6197 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 774 457 385 367 346 329 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2266 2589 2999 3317 513 Size: 552 939 262 323 410 318 3398 Fragments arranged by size: 3398 939 552 410 323 318 262 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2383 487 Size: 683 56 1157 4306 Fragments arranged by size: 4306 1157 683 56 BtsI GCAGTG_nn' Cuts at: 3282 3718 4512 4532 3282 Size: 436 794 20 4952 Fragments arranged by size: 4952 794 436 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 3649 4538 Size: 54 1425 50 275 18 486 1333 889 Cuts at: 4538 6167 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1333 889 486 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2101 2448 3026 4132 1353 Size: 748 347 578 1106 3423 Fragments arranged by size: 3423 1106 748 578 347 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2978 4789 5163 5309 387 Size: 1064 138 1389 1811 374 146 1280 Fragments arranged by size: 1811 1389 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2111 2779 2111 Size: 668 5534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2477 2619 2911 3374 4265 4862 Size: 393 713 132 142 292 463 891 597 Cuts at: 4862 5649 5917 1239 Size: 787 268 1524 Fragments arranged by size: 1524 891 787 713 597 463 393 292 268 142 132 EcoO109I rG'GnC_Cy Cuts at: 2594 2935 2594 Size: 341 5861 EcoRI G'AATT_C Cuts at: 3511 3889 3511 Size: 378 5824 EcoRV GAT'ATC Cuts at: 3232 3232 Size: 6202 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3037 3037 Size: 6202 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 5349 1678 Size: 3671 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2148 2180 4843 4875 4917 Size: 32 135 32 576 32 2663 32 42 Cuts at: 4917 4949 1373 Size: 32 2626 Fragments arranged by size: 2663 2626 576 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3546 3792 669 Size: 988 281 1608 246 3079 Fragments arranged by size: 3079 1608 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3792 3792 Size: 6202 KpnI G_GTAC'C Cuts at: 3527 3527 Size: 6202 MfeI C'AATT_G Cuts at: 3801 3801 Size: 6202 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5316 5500 1305 Size: 4011 184 2007 Fragments arranged by size: 4011 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4380 Fragments arranged by size: 4380 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4160 4519 4678 518 Size: 648 2994 359 159 2042 Fragments arranged by size: 2994 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2591 2964 3001 3618 4295 5443 5688 1606 Size: 985 373 37 617 677 1148 245 2120 Fragments arranged by size: 2120 1148 985 677 617 373 245 37 NaeI GCC'GGC Cuts at: 2337 2536 2884 2337 Size: 199 348 5655 Fragments arranged by size: 5655 348 199 NcoI C'CATG_G Cuts at: 513 1065 2004 3317 513 Size: 552 939 1313 3398 Fragments arranged by size: 3398 1313 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2335 2534 2882 2335 Size: 199 348 5655 Fragments arranged by size: 5655 348 199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NsiI A_TGCA'T Cuts at: 2051 2051 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 3560 5105 1132 Size: 97 2331 1545 2229 Fragments arranged by size: 2331 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6202 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 3291 1108 Size: 2183 4019 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PmlI CAC'GTG Cuts at: 2521 2521 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4271 4303 5784 5816 4271 Size: 32 1481 32 4657 Fragments arranged by size: 4657 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2594 2594 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 2013 1613 Size: 400 5802 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PstI C_TGCA'G Cuts at: 830 3554 830 Size: 2724 3478 PvuI CG_AT'CG Cuts at: 2001 2849 2001 Size: 848 5354 SacI G_AGCT'C Cuts at: 2113 2781 2113 Size: 668 5534 SacII CC_GC'GG Cuts at: 2592 3002 2592 Size: 410 5792 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SapI GCTCTTCn'nnn_ Cuts at: 2101 2448 3026 2101 Size: 347 578 5277 Fragments arranged by size: 5277 578 347 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 4450 6162 4450 Size: 1712 4490 SfcI C'TryA_G Cuts at: 826 1045 2782 3550 4685 5366 5557 826 Size: 219 1737 768 1135 681 191 1471 Fragments arranged by size: 1737 1471 1135 768 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 2678 3529 1872 Size: 806 851 4545 Fragments arranged by size: 4545 851 806 SmlI C'TyrA_G Cuts at: 820 1017 1943 2112 4306 5207 5469 820 Size: 197 926 169 2194 901 262 1553 Fragments arranged by size: 2194 1553 926 901 262 197 169 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 4126 5 Size: 47 4074 2081 Fragments arranged by size: 4074 2081 47 StuI AGG'CCT Cuts at: 1517 3345 1517 Size: 1828 4374 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 3317 3641 513 Size: 552 939 67 1246 324 3074 Fragments arranged by size: 3074 1246 939 552 324 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 2834 4229 4246 4399 4584 5029 Size: 1255 921 333 1395 17 153 185 445 Cuts at: 5029 325 Size: 1498 Fragments arranged by size: 1498 1395 1255 921 445 333 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4448 6160 96 Size: 274 80 33 51 1443 2471 1712 138 Fragments arranged by size: 2471 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6162 6194 6162 Size: 32 6170 Tth111I GACn'n_nGTC Cuts at: 1316 3299 1316 Size: 1983 4219 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 2676 3527 1870 Size: 806 851 4545 Fragments arranged by size: 4545 851 806 XmnI GAAnn'nnTTC Cuts at: 2924 4331 2924 Size: 1407 4795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI