Restriction map for
pFN21AB5895
(Linear) (Six-base) MAPSORT of: KIBB5895.seq Check: 4772 from: 1 to: 1497
KIBB5895.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 906 1369 1497
Size: 340 566 463 128
Fragments arranged by size:
566 463 340 128
AflIII A'CryG_T
Cuts at: 0 209 513 515 1497
Size: 209 304 2 982
Fragments arranged by size:
982 304 209 2
AlwNI CAG_nnn'CTG
Cuts at: 0 933 1497
Size: 933 564
ApaLI G'TGCA_C
Cuts at: 0 509 847 1497
Size: 509 338 650
Fragments arranged by size:
650 509 338
ApoI r'AATT_y
Cuts at: 0 1154 1497
Size: 1154 343
AvaI C'yCGr_G
Cuts at: 0 671 1497
Size: 671 826
BanII G_rGCy'C
Cuts at: 0 108 745 776 1035 1497
Size: 108 637 31 259 462
Fragments arranged by size:
637 462 259 108 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 1357 1497
Size: 151 1206 140
Fragments arranged by size:
1206 151 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 411 445 454 488 522 556 1497
Size: 411 34 9 34 34 34 941
Fragments arranged by size:
941 411 34 34 34 34 9
BclI T'GATC_A
Cuts at: 0 1238 1244 1497
Size: 1238 6 253
Fragments arranged by size:
1238 253 6
BfrBI ATG'CAT
Cuts at: 0 44 1497
Size: 44 1453
BglI GCCn_nnn'nGGC
Cuts at: 0 419 1337 1497
Size: 419 918 160
Fragments arranged by size:
918 419 160
Bme1580I G_kGCm'C
Cuts at: 0 66 513 612 851 1497
Size: 66 447 99 239 646
Fragments arranged by size:
646 447 239 99 66
BmgBI CAC'GTC
Cuts at: 0 981 1497
Size: 981 516
BmrI ACTGGGnnnn_n'
Cuts at: 0 1029 1497
Size: 1029 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 614 1497
Size: 472 142 883
Fragments arranged by size:
883 472 142
Bpu10I CC'TnA_GC
Cuts at: 0 688 940 1497
Size: 688 252 557
Fragments arranged by size:
688 557 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1497
Size: 92 1405
BsaAI yAC'GTr
Cuts at: 0 516 1497
Size: 516 981
BsaBI GATnn'nnATC
Cuts at: 0 193 256 1243 1497
Size: 193 63 987 254
Fragments arranged by size:
987 254 193 63
BsaHI Gr'CG_yC
Cuts at: 0 1096 1497
Size: 1096 401
BsaWI w'CCGG_w
Cuts at: 0 204 443 1497
Size: 204 239 1054
Fragments arranged by size:
1054 239 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 1497
Size: 727 30 740
Fragments arranged by size:
740 727 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 1497
Size: 225 1272
BseYI C'CCAG_C
Cuts at: 0 335 613 791 969 1379 1497
Size: 335 278 178 178 410 118
Fragments arranged by size:
410 335 278 178 178 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1289 1497
Size: 856 433 208
Fragments arranged by size:
856 433 208
BsiEI CG_ry'CG
Cuts at: 0 844 1497
Size: 844 653
BsiHKAI G_wGCw'C
Cuts at: 0 108 513 680 776 851 1321 1497
Size: 108 405 167 96 75 470 176
Fragments arranged by size:
470 405 176 167 108 96 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1087 1497
Size: 1087 410
Bsp1286I G_dGCh'C
Cuts at: 0 66 108 513 612 680 745 776 851
Size: 66 42 405 99 68 65 31 75
Cuts at: 851 1035 1321 1497
Size: 184 286 176
Fragments arranged by size:
405 286 184 176 99 75 68 66
65 42 31
BspEI T'CCGG_A
Cuts at: 0 204 1497
Size: 204 1293
BspHI T'CATG_A
Cuts at: 0 1241 1497
Size: 1241 256
BsrFI r'CCGG_y
Cuts at: 0 330 529 877 1497
Size: 330 199 348 620
Fragments arranged by size:
620 348 330 199
BssSI C'ACGA_G
Cuts at: 0 835 1497
Size: 835 662
BstBI TT'CG_AA
Cuts at: 0 192 1497
Size: 192 1305
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 648 1167 1497
Size: 33 615 519 330
Fragments arranged by size:
615 519 330 33
BstYI r'GATC_y
Cuts at: 0 296 1070 1497
Size: 296 774 427
Fragments arranged by size:
774 427 296
BtgI C'CryG_G
Cuts at: 0 261 584 994 1312 1497
Size: 261 323 410 318 185
Fragments arranged by size:
410 323 318 261 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 378 1497
Size: 378 1119
BtsI GCAGTG_nn'
Cuts at: 0 1277 1497
Size: 1277 220
EaeI y'GGCC_r
Cuts at: 0 311 1497
Size: 311 1186
EarI CTCTTCn'nnn_
Cuts at: 0 96 443 1021 1497
Size: 96 347 578 476
Fragments arranged by size:
578 476 347 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1497
Size: 973 524
EcoICRI GAG'CTC
Cuts at: 0 106 774 1497
Size: 106 668 723
Fragments arranged by size:
723 668 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 472 614 906 1369 1497
Size: 340 132 142 292 463 128
Fragments arranged by size:
463 340 292 142 132 128
EcoO109I rG'GnC_Cy
Cuts at: 0 589 930 1497
Size: 589 341 567
Fragments arranged by size:
589 567 341
EcoRV GAT'ATC
Cuts at: 0 1227 1497
Size: 1227 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1032 1497
Size: 1032 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 1497
Size: 143 32 1322
Fragments arranged by size:
1322 143 32
MspA1I CmG'CkG
Cuts at: 0 586 959 996 1497
Size: 586 373 37 501
Fragments arranged by size:
586 501 373 37
NaeI GCC'GGC
Cuts at: 0 332 531 879 1497
Size: 332 199 348 618
Fragments arranged by size:
618 348 332 199
NcoI C'CATG_G
Cuts at: 0 1312 1497
Size: 1312 185
NgoMIV G'CCGG_C
Cuts at: 0 330 529 877 1497
Size: 330 199 348 620
Fragments arranged by size:
620 348 330 199
NsiI A_TGCA'T
Cuts at: 0 46 1497
Size: 46 1451
PflMI CCAn_nnn'nTGG
Cuts at: 0 1286 1497
Size: 1286 211
PmlI CAC'GTG
Cuts at: 0 516 1497
Size: 516 981
PpuMI rG'GwC_Cy
Cuts at: 0 589 1497
Size: 589 908
PshAI GACnn'nnGTC
Cuts at: 0 8 1497
Size: 8 1489
PvuI CG_AT'CG
Cuts at: 0 844 1497
Size: 844 653
SacI G_AGCT'C
Cuts at: 0 108 776 1497
Size: 108 668 721
Fragments arranged by size:
721 668 108
SacII CC_GC'GG
Cuts at: 0 587 997 1497
Size: 587 410 500
Fragments arranged by size:
587 500 410
SapI GCTCTTCn'nnn_
Cuts at: 0 96 443 1021 1497
Size: 96 347 578 476
Fragments arranged by size:
578 476 347 96
SfcI C'TryA_G
Cuts at: 0 777 1497
Size: 777 720
SmaI CCC'GGG
Cuts at: 0 673 1497
Size: 673 824
SmlI C'TyrA_G
Cuts at: 0 107 1497
Size: 107 1390
StuI AGG'CCT
Cuts at: 0 1340 1497
Size: 1340 157
StyI C'CwwG_G
Cuts at: 0 66 1312 1497
Size: 66 1246 185
Fragments arranged by size:
1246 185 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 829 1497
Size: 496 333 668
Fragments arranged by size:
668 496 333
Tth111I GACn'n_nGTC
Cuts at: 0 1294 1497
Size: 1294 203
XmaI C'CCGG_G
Cuts at: 0 671 1497
Size: 671 826
XmnI GAAnn'nnTTC
Cuts at: 0 919 1497
Size: 919 578
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI AvaI BanII BbsI
BcgI BclI BfrBI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BsrFI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI
Hin4I MspA1I NaeI NcoI NgoMIV NsiI PflMI PmlI
PpuMI PshAI PvuI SacI SacII SapI SfcI SmaI
SmlI StuI StyI TaqII Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BglII
BlpI BmtI BplI BsaI BsiWI BsmI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NdeI NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PstI PvuII RsrII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XcmI XhoI ZraI