Restriction map for
pFN21AB5876
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HincII
HindII HpaI HphI KasI KpnI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmlI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XcmI XhoI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccII 98 CGCG CG^CG
AccIII 1380 TCCGGA T^CCGGA
AclI 1326 AACGTT AA^CGTT
AfaI 1425 GTAC GT^AC
AflIII 575 ACATGT A^C[GA][CT]GT
AhdI 280 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 923 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 73 GGGCCC GGGCC^C
AspEI 280 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1196 GTGCTC G[AT]GC[AT]^C
AvaII 967 GGTCC G^G[AT]CC
BalI 749 TGGCCA TGG^CCA
BbsI 366 GTCTTC GAAGAC(2/6)
BcgI 1413 GCAGTGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1455 GGATAC GTATCC(6/5)
BlpI 4 GCTGAGC GC^T[ACGT]AGC
BpmI 695 CTCCAG CTGGAG(16/14)
Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC
BpuAI 366 GTCTTC GAAGAC(2/6)
BseAI 1380 TCCGGA T^CCGGA
BsgI 620 CTGCAC GTGCAG(16/14)
BsiHKAI 1196 GTGCTC G[AT]GC[AT]^C
BsmBI 47 CGTCTC CGTCTC(1/5)
BsmFI 101 GGGAC GGGAC(10/14)
BspEI 1380 TCCGGA T^CCGGA
BspLU11I 575 ACATGT A^CATGT
BsrDI 121 GCAATG GCAATG(2/0)
BssSI 625 CACGAG CACGAG(-5/-1)
BstBI 1333 TTCGAA TT^CGAA
BstUI 98 CGCG CG^CG
BstYI 987 AGATCC [GA]^GATC[CT]
CelII 4 GCTGAGC GC^T[ACGT]AGC
CfoI 265 GCGC GCG^C
DraI 547 TTTAAA TTT^AAA
DraIII 345 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 280 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 792 GAAGAG CTCTTC(1/4)
EcoT22I 658 ATGCAT ATGCA^T
HhaI 265 GCGC GCG^C
HinP1I 265 GCGC G^CGC
HindIII 1275 AAGCTT A^AGCTT
Ksp632I 792 GAAGAG CTCTTC(1/4)
KspI 97 CCGCGG CCGC^GG
MflI 987 AGATCC [GA]^GATC[CT]
MluNI 749 TGGCCA TGG^CCA
MroI 1380 TCCGGA T^CCGGA
MscI 749 TGGCCA TGG^CCA
MslI 943 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 98 CGCG CG^CG
MwoI 129 GCCGCCACCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 203 CCATGG C^CATGG
NsiI 658 ATGCAT ATGCA^T
NspV 1333 TTCGAA TT^CGAA
PflMI 344 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1326 AACGTT AA^CGTT
PstI 407 CTGCAG CTGCA^G
RsaI 1425 GTAC GT^AC
SacII 97 CCGCGG CCGC^GG
SexAI 1178 ACCAGGT A^CC[AT]GGT
SfcI 407 CTGCAG C^T[GA][CT]AG
SfuI 1333 TTCGAA TT^CGAA
SpeI 13 ACTAGT A^CTAGT
SspI 754 AATATT AAT^ATT
Van91I 344 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 910 TCTAGA T^CTAGA
XhoII 987 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 244 GACGCC G[GA]^CG[CT]C
280 GACGCC
AlwI 988 GATCC GGATC(4/5)
931 GGATC
BanII 73 GGGCCC G[GA]GC[CT]^C
227 GGGCTC
BsaHI 244 GACGCC G[GA]^CG[CT]C
280 GACGCC
BsaWI 972 ACCGGA [AT]^CCGG[AT]
1380 TCCGGA
BseRI 797 GAGGAG GAGGAG(10/8)
1033 GAGGAG
DraII 72 AGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGCCCC
DsaI 97 CCGCGG C^C[GA][CT]GG
203 CCATGG
EaeI 318 CGGCCA [CT]^GGCC[GA]
749 TGGCCA
EcoO109I 72 AGGGCCC [GA]G^G[ACGT]CC[CT]
73 GGGCCCC
Hin1I 244 GACGCC G[GA]^CG[CT]C
280 GACGCC
MspA1I 97 CCGCGG C[AC]G^C[GT]G
315 CAGCGG
SfaNI 442 GATGC GCATC(5/9)
387 GCATC
SmaI 223 CCCGGG CCC^GGG
259 CCCGGG
SphI 42 GCATGC GCATG^C
660 GCATGC
XmaCI 223 CCCGGG C^CCGGG
259 CCCGGG
XmaI 223 CCCGGG C^CCGGG
259 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AvaI BbvI BcnI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI NciI
NdeII NlaIII NlaIV NspI PleI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI