(Circular) (Six-base) MAPSORT of: pFN21AB5876.seq Check: 7323 from: 1 to: 6190 pFN21AB5876 6190 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 3332 4317 4690 1563 Size: 1769 985 373 3063 Fragments arranged by size: 3063 1769 985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2449 3180 4253 5637 5905 1632 Size: 817 731 1073 1384 268 1917 Fragments arranged by size: 1917 1384 1073 817 731 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 2580 5089 6066 1203 Size: 1377 2509 977 1327 Fragments arranged by size: 2509 1377 1327 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 2290 4919 2290 Size: 2629 3561 AleI CACnn'nnGTG Cuts at: 1166 2952 1166 Size: 1786 4404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3340 3372 3340 Size: 32 6158 AlwNI CAG_nnn'CTG Cuts at: 2933 3933 5505 2933 Size: 1000 1572 3618 Fragments arranged by size: 3618 1572 1000 ApaI G_GGCC'C Cuts at: 2082 2082 Size: 6190 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 2335 2422 2453 3244 3499 3725 3877 5986 Size: 842 87 31 791 255 226 152 2109 Cuts at: 5986 1493 Size: 1697 Fragments arranged by size: 2109 1697 842 791 255 226 152 87 31 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2228 2264 3515 1870 Size: 73 114 171 36 1251 4545 Fragments arranged by size: 4545 1251 171 114 73 36 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3511 4966 618 Size: 325 217 627 1724 1455 1842 Fragments arranged by size: 1842 1724 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2082 2236 3509 1391 Size: 691 154 1273 4072 Fragments arranged by size: 4072 1273 691 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 4754 Fragments arranged by size: 4754 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3407 3441 4381 4415 1903 Size: 34 1470 34 940 34 3678 Fragments arranged by size: 3678 1470 940 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3454 4082 5292 3454 Size: 628 1210 4352 Fragments arranged by size: 4352 1210 628 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BfrBI ATG'CAT Cuts at: 2665 2665 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 2010 3607 2010 Size: 1597 4593 Bme1580I G_kGCm'C Cuts at: 1790 2082 4251 5407 1790 Size: 292 2169 1156 2573 Fragments arranged by size: 2573 2169 1156 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2212 2284 2472 Size: 416 53 83 399 24 795 72 188 Cuts at: 2472 3921 4879 442 Size: 1449 958 1753 Fragments arranged by size: 1753 1449 958 795 416 399 188 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 4850 1239 Size: 1446 2165 2579 Fragments arranged by size: 2579 2165 1446 Bpu10I CC'TnA_GC Cuts at: 2084 2084 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4315 5180 5478 4315 Size: 865 298 5027 Fragments arranged by size: 5027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 2286 3997 4379 Size: 53 83 186 936 717 36 1711 382 Cuts at: 4379 5014 275 Size: 635 1451 Fragments arranged by size: 1711 1451 936 717 635 382 186 83 53 36 BsaWI w'CCGG_w Cuts at: 2977 3385 4623 5021 5295 5442 5853 2977 Size: 408 1238 398 274 147 411 3314 Fragments arranged by size: 3314 1238 411 408 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 5134 Fragments arranged by size: 5134 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2817 3053 1186 Size: 1631 236 4323 Fragments arranged by size: 4323 1631 236 BseYI C'CCAG_C Cuts at: 2318 5393 5889 2318 Size: 3075 496 2619 Fragments arranged by size: 3075 2619 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2610 1163 Size: 573 874 4743 Fragments arranged by size: 4743 874 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3205 4251 4336 5407 3205 Size: 1046 85 1071 3988 Fragments arranged by size: 3988 1071 1046 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 BsmBI CGTCTCn'nnnn_ Cuts at: 2058 2058 Size: 6190 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2236 3205 3509 4251 4336 5407 Size: 399 292 154 969 304 742 85 1071 Cuts at: 5407 1391 Size: 2174 Fragments arranged by size: 2174 1071 969 742 399 304 292 154 85 BspEI T'CCGG_A Cuts at: 3385 5853 3385 Size: 2468 3722 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3545 844 Size: 2701 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2133 4685 4859 65 Size: 1183 885 2552 174 1396 Fragments arranged by size: 2552 1396 1183 885 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4834 5021 1700 Size: 110 3024 187 2869 Fragments arranged by size: 3024 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 2630 4250 5262 2630 Size: 1620 1012 3558 Fragments arranged by size: 3558 1620 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 3339 4994 3339 Size: 1655 4535 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2992 3520 3887 4272 4289 5741 5827 5839 Size: 1020 528 367 385 17 1452 86 12 Cuts at: 5839 6185 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1020 528 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2102 2208 513 Size: 552 939 98 106 4495 Fragments arranged by size: 4495 939 552 106 98 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 2091 3205 3425 3706 4500 4520 2091 Size: 1114 220 281 794 20 3761 Fragments arranged by size: 3761 1114 794 281 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 2554 3494 2554 Size: 940 5250 DraIII CAC_nnn'GTG Cuts at: 2355 2355 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2323 2754 3637 Size: 54 1425 50 275 18 493 431 883 Cuts at: 3637 4526 6155 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 883 493 431 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2792 4120 1353 Size: 1439 1328 3423 Fragments arranged by size: 3423 1439 1328 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2226 4777 5151 5297 387 Size: 1064 138 637 2551 374 146 1280 Fragments arranged by size: 2551 1280 1064 637 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2449 2685 3180 4253 4850 5637 5905 Size: 393 817 236 495 1073 597 787 268 Cuts at: 5905 1239 Size: 1524 Fragments arranged by size: 1524 1073 817 787 597 495 393 268 236 EcoO109I rG'GnC_Cy Cuts at: 2078 2079 2078 Size: 1 6189 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3298 3298 Size: 6190 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2276 2308 2863 Size: 32 135 32 632 32 40 32 555 Cuts at: 2863 2895 3011 3043 3202 3234 4831 4863 4905 Size: 32 116 32 159 32 1597 32 42 Cuts at: 4905 4937 1373 Size: 32 2626 Fragments arranged by size: 2626 1597 632 555 159 135 116 42 40 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 3280 748 Size: 2532 3658 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2771 5304 5488 1305 Size: 1466 2533 184 2007 Fragments arranged by size: 2533 2007 1466 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 3444 Fragments arranged by size: 3444 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2952 4148 4507 4666 518 Size: 648 1786 1196 359 159 2042 Fragments arranged by size: 2042 1786 1196 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2322 3606 4283 5431 5676 1606 Size: 498 218 1284 677 1148 245 2120 Fragments arranged by size: 2120 1284 1148 677 498 245 218 NcoI C'CATG_G Cuts at: 513 1065 2004 2208 513 Size: 552 939 204 4495 Fragments arranged by size: 4495 939 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NsiI A_TGCA'T Cuts at: 2667 2667 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2051 2584 2669 3548 5093 1132 Size: 97 822 533 85 879 1545 2229 Fragments arranged by size: 2229 1545 879 822 533 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 2580 5089 2580 Size: 2509 3681 PflMI CCAn_nnn'nTGG Cuts at: 1108 2355 1108 Size: 1247 4943 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 2078 2078 Size: 6190 PstI C_TGCA'G Cuts at: 830 2416 3542 830 Size: 1586 1126 3478 Fragments arranged by size: 3478 1586 1126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 SacII CC_GC'GG Cuts at: 2105 2105 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SexAI A'CCwGG_T Cuts at: 3183 3183 Size: 6190 SfcI C'TryA_G Cuts at: 826 1045 2412 3538 4673 5354 5545 826 Size: 219 1367 1126 1135 681 191 1471 Fragments arranged by size: 1471 1367 1135 1126 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 2230 2266 3517 1872 Size: 358 36 1251 4545 Fragments arranged by size: 4545 1251 358 36 SmlI C'TyrA_G Cuts at: 820 1017 1943 4294 5195 5457 820 Size: 197 926 2351 901 262 1553 Fragments arranged by size: 2351 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 2018 152 Size: 1866 4324 SphI G_CATG'C Cuts at: 1132 2051 2669 3548 1132 Size: 919 618 879 3774 Fragments arranged by size: 3774 919 879 618 SspI AAT'ATT Cuts at: 5 52 2761 4114 5 Size: 47 2709 1353 2081 Fragments arranged by size: 2709 2081 1353 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2004 2208 2330 3066 3629 513 Size: 552 939 204 122 736 563 3074 Fragments arranged by size: 3074 939 736 563 552 204 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4217 4234 4387 4572 5017 325 Size: 1255 2637 17 153 185 445 1498 Fragments arranged by size: 2637 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4436 6148 96 Size: 274 80 33 51 1443 2459 1712 138 Fragments arranged by size: 2459 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6150 6182 6150 Size: 32 6158 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 2915 3526 2915 Size: 611 5579 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 2228 2264 3515 1870 Size: 358 36 1251 4545 Fragments arranged by size: 4545 1251 358 36 XmnI GAAnn'nnTTC Cuts at: 2402 3247 3375 4319 2402 Size: 845 128 944 4273 Fragments arranged by size: 4273 944 845 128 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI