(Circular) (Six-base) MAPSORT of: pFN21AB7813.seq Check: 8278 from: 1 to: 6301 pFN21AB7813 6301 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2370 4111 278 Size: 53 83 186 936 834 1741 2468 Fragments arranged by size: 2468 1741 936 834 186 83 53 AccI GT'mk_AC Cuts at: 1937 3196 3644 1937 Size: 1259 448 4594 Fragments arranged by size: 4594 1259 448 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3078 3404 4364 5748 6016 1632 Size: 1446 326 960 1384 268 1917 Fragments arranged by size: 1917 1446 1384 960 326 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2054 5200 6177 1203 Size: 851 3146 977 1327 Fragments arranged by size: 3146 1327 977 851 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 2813 3274 5030 2813 Size: 461 1756 4084 Fragments arranged by size: 4084 1756 461 AleI CACnn'nnGTG Cuts at: 1166 2427 1166 Size: 1261 5040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2424 2456 2424 Size: 32 6269 AlwNI CAG_nnn'CTG Cuts at: 4044 5616 4044 Size: 1572 4729 ApaI G_GGCC'C Cuts at: 2394 2394 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2164 3610 3836 3988 6097 1493 Size: 671 1446 226 152 2109 1697 Fragments arranged by size: 2109 1697 1446 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2794 3622 5077 618 Size: 325 217 627 1007 828 1455 1842 Fragments arranged by size: 1842 1455 1007 828 627 325 217 BanII G_rGCy'C Cuts at: 1391 2394 3380 3620 1391 Size: 1003 986 240 4072 Fragments arranged by size: 4072 1003 986 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3394 928 Size: 971 1495 3835 Fragments arranged by size: 3835 1495 971 BbvCI CC'TCA_GC Cuts at: 3012 3012 Size: 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4492 4526 1903 Size: 34 50 34 2471 34 3678 Fragments arranged by size: 3678 2471 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3349 4193 5403 3349 Size: 844 1210 4247 Fragments arranged by size: 4247 1210 844 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 2949 3079 3718 2949 Size: 130 639 5532 Fragments arranged by size: 5532 639 130 Bme1580I G_kGCm'C Cuts at: 1790 2394 4362 5518 1790 Size: 604 1968 1156 2573 Fragments arranged by size: 2573 1968 1156 604 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3062 4032 4990 Size: 416 53 83 399 24 1645 970 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 1753 1645 970 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2532 2672 2675 4961 1239 Size: 1293 140 3 2286 2579 Fragments arranged by size: 2579 2286 1293 140 3 Bpu10I CC'TnA_GC Cuts at: 2216 3012 2216 Size: 796 5505 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2098 4426 5291 5589 2098 Size: 2328 865 298 2810 Fragments arranged by size: 2810 2328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2800 4964 882 Size: 617 1301 2164 2219 Fragments arranged by size: 2219 2164 1301 617 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 2565 4108 4490 Size: 53 83 186 936 834 198 1543 382 Cuts at: 4490 5125 275 Size: 635 1451 Fragments arranged by size: 1543 1451 936 834 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3352 3382 1178 Size: 30 165 30 1949 30 4097 Fragments arranged by size: 4097 1949 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2672 1186 Size: 1486 4815 BseYI C'CCAG_C Cuts at: 2711 3221 3322 5504 6000 2711 Size: 510 101 2182 496 3012 Fragments arranged by size: 3012 2182 510 496 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5728 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2664 3447 3591 4362 4447 5518 2664 Size: 783 144 771 85 1071 3447 Fragments arranged by size: 3447 1071 783 771 144 85 BsmI GAATG_Cn' Cuts at: 3527 3812 3905 3527 Size: 285 93 5923 Fragments arranged by size: 5923 285 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2664 3380 3447 3591 3620 4362 Size: 399 604 270 716 67 144 29 742 Cuts at: 4362 4447 5518 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 604 399 270 144 85 67 29 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 2721 3148 4190 5920 2721 Size: 427 1042 1730 3102 Fragments arranged by size: 3102 1730 1042 427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2707 2818 3656 844 Size: 1863 111 838 3489 Fragments arranged by size: 3489 1863 838 111 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2008 4796 4970 65 Size: 1183 760 2788 174 1396 Fragments arranged by size: 2788 1396 1183 760 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3477 96 Size: 3381 2920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 2382 3231 794 Size: 1588 849 3864 Fragments arranged by size: 3864 1588 849 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 3490 3490 Size: 6301 BstYI r'GATC_y Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296 Size: 1659 367 385 17 1452 86 12 346 Cuts at: 6296 1972 Size: 1977 Fragments arranged by size: 1977 1659 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2582 3372 2582 Size: 790 5511 BtgI C'CryG_G Cuts at: 513 1065 3499 513 Size: 552 2434 3315 Fragments arranged by size: 3315 2434 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 3445 3817 4611 4631 3445 Size: 372 794 20 5115 Fragments arranged by size: 5115 794 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2605 2640 3514 3549 529 Size: 35 2041 35 874 35 3281 Fragments arranged by size: 3281 2041 874 35 35 35 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3192 5308 809 Size: 507 1876 2116 1802 Fragments arranged by size: 2116 1876 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266 Size: 54 1425 50 275 18 1918 889 1629 Cuts at: 6266 8 Size: 43 Fragments arranged by size: 1918 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2190 4231 1353 Size: 837 2041 3423 Fragments arranged by size: 3423 2041 837 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2672 2675 3078 3404 4364 4961 Size: 393 900 140 3 403 326 960 597 Cuts at: 4961 5748 6016 1239 Size: 787 268 1524 Fragments arranged by size: 1524 960 900 787 597 403 393 326 268 140 3 EcoO109I rG'GnC_Cy Cuts at: 2586 3016 3429 3511 2586 Size: 430 413 82 5376 Fragments arranged by size: 5376 430 413 82 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3099 3342 3099 Size: 243 6058 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2799 2831 2947 Size: 32 135 32 524 32 671 32 116 Cuts at: 2947 2979 3260 3292 3551 3583 4942 4974 5016 Size: 32 281 32 259 32 1359 32 42 Cuts at: 5016 5048 1373 Size: 32 2626 Fragments arranged by size: 2626 1359 671 524 281 259 135 116 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3197 3645 3891 669 Size: 988 281 1259 448 246 3079 Fragments arranged by size: 3079 1259 988 448 281 246 HindIII A'AGCT_T Cuts at: 748 3348 748 Size: 2600 3701 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 2100 3900 2100 Size: 1800 4501 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5415 5599 1305 Size: 4110 184 2007 Fragments arranged by size: 4110 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4479 Fragments arranged by size: 4479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2427 3488 3520 4259 4618 4777 518 Size: 648 1261 1061 32 739 359 159 2042 Fragments arranged by size: 2042 1261 1061 739 648 359 159 32 MspA1I CmG'CkG Cuts at: 1606 3717 4394 5542 5787 1606 Size: 2111 677 1148 245 2120 Fragments arranged by size: 2120 2111 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3499 513 Size: 552 2434 3315 Fragments arranged by size: 3315 2434 552 NdeI CA'TA_TG Cuts at: 387 2935 2967 387 Size: 2548 32 3721 Fragments arranged by size: 3721 2548 32 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2138 3659 5204 1132 Size: 97 909 1521 1545 2229 Fragments arranged by size: 2229 1545 1521 909 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 1108 Size: 1488 4813 PfoI T'CCnGG_A Cuts at: 2310 2310 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2507 2539 4370 4402 5883 5915 2507 Size: 32 1831 32 1481 32 2893 Fragments arranged by size: 2893 1831 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2586 3511 2586 Size: 925 5376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3074 3871 3074 Size: 797 5504 PspOMI G'GGCC_C Cuts at: 2390 2390 Size: 6301 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3195 3643 1936 Size: 1259 448 4594 Fragments arranged by size: 4594 1259 448 SanDI GG'GwC_CC Cuts at: 3511 3511 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2539 2551 3538 3649 4784 5465 5656 Size: 219 1494 12 987 111 1135 681 191 Cuts at: 5656 826 Size: 1471 Fragments arranged by size: 1494 1471 1135 987 681 219 191 111 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2113 4405 5306 5568 820 Size: 197 926 170 2292 901 262 1553 Fragments arranged by size: 2292 1553 926 901 262 197 170 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 2138 3659 1132 Size: 1006 1521 3774 Fragments arranged by size: 3774 1521 1006 SspI AAT'ATT Cuts at: 5 52 2785 4225 5 Size: 47 2733 1440 2081 Fragments arranged by size: 2733 2081 1440 47 StuI AGG'CCT Cuts at: 1517 3215 1517 Size: 1698 4603 StyI C'CwwG_G Cuts at: 513 1065 3004 3283 3363 3499 3740 513 Size: 552 1939 279 80 136 241 3074 Fragments arranged by size: 3074 1939 552 279 241 136 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 4328 4345 4498 4683 5128 325 Size: 1255 557 2191 17 153 185 445 1498 Fragments arranged by size: 2191 1498 1255 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3477 4547 6259 Size: 274 80 33 51 1443 1500 1070 1712 Cuts at: 6259 96 Size: 138 Fragments arranged by size: 1712 1500 1443 1070 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 3470 1316 Size: 2154 4147 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2401 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2522 4430 2522 Size: 1908 4393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2368 4109 276 Size: 53 83 186 936 834 1741 2468 Fragments arranged by size: 2468 1741 936 834 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI