(Circular) (Six-base) MAPSORT of: pFN21AB7714.seq Check: 6915 from: 1 to: 6124 pFN21AB7714 6124 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2062 3047 2062 Size: 985 5139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2896 2969 3131 3260 3314 4187 5571 5839 Size: 1264 73 162 129 54 873 1384 268 Cuts at: 5839 1632 Size: 1917 Fragments arranged by size: 1917 1384 1264 873 268 162 129 73 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2291 2856 2868 5023 6000 1203 Size: 1088 565 12 2155 977 1327 Fragments arranged by size: 2155 1327 1088 977 565 12 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 2182 4853 2182 Size: 2671 3453 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2091 1184 Size: 907 5217 AlwNI CAG_nnn'CTG Cuts at: 2186 2533 2548 2664 2787 3352 3867 5439 2186 Size: 347 15 116 123 565 515 1572 2871 Fragments arranged by size: 2871 1572 565 515 347 123 116 15 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2632 3095 3433 3659 3811 5920 1493 Size: 1139 463 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 1139 463 338 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 3454 3821 3454 Size: 367 5757 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2349 2366 3445 4900 618 Size: 325 217 627 562 17 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 562 325 217 17 BanII G_rGCy'C Cuts at: 1391 3087 3321 3443 1391 Size: 1696 234 122 4072 Fragments arranged by size: 4072 1696 234 122 BbeI G_GCGC'C Cuts at: 2370 2370 Size: 6124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2393 3054 928 Size: 971 494 661 3998 Fragments arranged by size: 3998 971 661 494 BbvCI CC'TCA_GC Cuts at: 2345 2345 Size: 6124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 2454 2493 2571 1548 Size: 906 39 78 5101 Fragments arranged by size: 5101 906 78 39 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2734 2826 2937 6119 2734 Size: 92 111 3182 2739 Fragments arranged by size: 3182 2739 111 92 BlpI GC'TnA_GC Cuts at: 2654 3336 3541 2654 Size: 682 205 5237 Fragments arranged by size: 5237 682 205 Bme1580I G_kGCm'C Cuts at: 1790 2354 4185 5341 1790 Size: 564 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 564 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 2543 3855 Size: 416 53 83 399 24 779 347 1312 Cuts at: 3855 4813 442 Size: 958 1753 Fragments arranged by size: 1753 1312 958 779 416 399 347 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2848 2848 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2450 3101 4784 1239 Size: 905 306 651 1683 2579 Fragments arranged by size: 2579 1683 905 651 306 Bpu10I CC'TnA_GC Cuts at: 2345 2345 Size: 6124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4249 5114 5412 4249 Size: 865 298 4961 Fragments arranged by size: 4961 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2125 2758 4787 882 Size: 617 626 633 2029 2219 Fragments arranged by size: 2219 2029 633 626 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 2570 6118 2570 Size: 3548 2576 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3931 4313 4948 Size: 53 83 186 936 834 1564 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1564 1451 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2358 2727 4557 4955 5229 5376 5787 2358 Size: 369 1830 398 274 147 411 2695 Fragments arranged by size: 2695 1830 411 398 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2924 2954 1178 Size: 30 165 30 1521 30 4348 Fragments arranged by size: 4348 1521 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2492 2570 3146 3149 1186 Size: 1306 78 576 3 4161 Fragments arranged by size: 4161 1306 576 78 3 BseYI C'CCAG_C Cuts at: 2803 3267 3345 5327 5823 2803 Size: 464 78 1982 496 3104 Fragments arranged by size: 3104 1982 496 464 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2051 2561 1163 Size: 573 315 510 4726 Fragments arranged by size: 4726 573 510 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2053 2744 3321 4185 4270 5341 2053 Size: 691 577 864 85 1071 2836 Fragments arranged by size: 2836 1071 864 691 577 85 BsmI GAATG_Cn' Cuts at: 3260 3635 3728 3260 Size: 375 93 5656 Fragments arranged by size: 5656 375 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2354 2744 3087 3321 3443 4185 Size: 399 263 301 390 343 234 122 742 Cuts at: 4185 4270 5341 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 343 301 263 234 122 85 BspEI T'CCGG_A Cuts at: 2358 2727 5787 2358 Size: 369 3060 2695 Fragments arranged by size: 3060 2695 369 BspHI T'CATG_A Cuts at: 2286 4013 5743 2286 Size: 1727 1730 2667 Fragments arranged by size: 2667 1730 1727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2062 3047 3479 844 Size: 1218 985 432 3489 Fragments arranged by size: 3489 1218 985 432 BsrBI CCG'CTC Cuts at: 1394 1823 2436 4011 1394 Size: 429 613 1575 3507 Fragments arranged by size: 3507 1575 613 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4619 4793 65 Size: 1183 3371 174 1396 Fragments arranged by size: 3371 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3027 4768 4955 1700 Size: 110 1217 1741 187 2869 Fragments arranged by size: 2869 1741 1217 187 110 BsrGI T'GTAC_A Cuts at: 96 2164 96 Size: 2068 4056 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 2664 794 Size: 1870 4254 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstYI r'GATC_y Cuts at: 1972 2734 2826 2937 3454 3821 4206 4223 5675 Size: 762 92 111 517 367 385 17 1452 Cuts at: 5675 5761 5773 6119 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 762 517 385 367 346 111 92 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2472 513 Size: 552 1407 4165 Fragments arranged by size: 4165 1407 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2665 2788 3051 3640 4434 4454 2665 Size: 123 263 589 794 20 4335 Fragments arranged by size: 4335 794 589 263 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DraIII CAC_nnn'GTG Cuts at: 3202 3202 Size: 6124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3025 3571 4460 Size: 54 1425 50 275 18 1195 546 889 Cuts at: 4460 6089 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1195 889 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3170 4054 1353 Size: 1817 884 3423 Fragments arranged by size: 3423 1817 884 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4711 5085 5231 387 Size: 1064 138 3122 374 146 1280 Fragments arranged by size: 3122 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3319 3319 Size: 6124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2450 2896 2969 3101 3131 3260 Size: 393 512 306 446 73 132 30 129 Cuts at: 3260 3314 4187 4784 5571 5839 1239 Size: 54 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 597 512 446 393 306 268 132 129 73 54 30 EcoNI CCTnn'n_nnAGG Cuts at: 2911 2948 3395 2911 Size: 37 447 5640 Fragments arranged by size: 5640 447 37 EcoO109I rG'GnC_Cy Cuts at: 2040 2231 2689 2040 Size: 191 458 5475 Fragments arranged by size: 5475 458 191 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 FspI TGC'GCA Cuts at: 2420 3359 4630 2420 Size: 939 1271 3914 Fragments arranged by size: 3914 1271 939 HaeII r_GCGC'y Cuts at: 1678 2370 5271 1678 Size: 692 2901 2531 Fragments arranged by size: 2901 2531 692 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2509 2541 2575 Size: 32 135 32 596 32 309 32 34 Cuts at: 2575 2607 2726 2758 2924 2956 4765 4797 4839 Size: 32 119 32 166 32 1809 32 42 Cuts at: 4839 4871 1373 Size: 32 2626 Fragments arranged by size: 2626 1809 596 309 166 135 119 42 34 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2209 2862 2975 3468 3714 669 Size: 988 281 271 653 113 493 246 3079 Fragments arranged by size: 3079 988 653 493 281 271 246 113 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KasI G'GCGC_C Cuts at: 2366 2366 Size: 6124 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2206 2487 5238 5422 1305 Size: 901 281 2751 184 2007 Fragments arranged by size: 2751 2007 901 281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4302 Fragments arranged by size: 4302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 3305 4082 4441 4600 518 Size: 648 1114 1025 777 359 159 2042 Fragments arranged by size: 2042 1114 1025 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2563 2764 3124 3214 3349 3540 4217 5365 Size: 957 201 360 90 135 191 677 1148 Cuts at: 5365 5610 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 957 677 360 245 201 191 135 90 NaeI GCC'GGC Cuts at: 3029 3029 Size: 6124 NarI GG'CG_CC Cuts at: 2367 2367 Size: 6124 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NgoMIV G'CCGG_C Cuts at: 3027 3027 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2295 2860 2872 3109 3482 5027 1132 Size: 97 1066 565 12 237 373 1545 2229 Fragments arranged by size: 2229 1545 1066 565 373 237 97 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 2291 2856 2868 5023 2291 Size: 565 12 2155 3392 Fragments arranged by size: 3392 2155 565 12 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 2191 3363 2191 Size: 1172 4952 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 2231 2040 Size: 191 5933 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 2688 2763 3058 3123 3195 3219 3476 830 Size: 1858 75 295 65 72 24 257 3478 Fragments arranged by size: 3478 1858 295 257 75 72 65 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 PvuII CAG'CTG Cuts at: 2764 3124 3214 2764 Size: 360 90 5674 Fragments arranged by size: 5674 360 90 SacI G_AGCT'C Cuts at: 3321 3321 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SanDI GG'GwC_CC Cuts at: 2040 2040 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SfcI C'TryA_G Cuts at: 826 1045 2684 2759 3054 3119 3191 3215 3472 Size: 219 1639 75 295 65 72 24 257 Cuts at: 3472 4607 5288 5479 826 Size: 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 681 295 257 219 191 75 72 65 24 SfoI GGC'GCC Cuts at: 2368 2368 Size: 6124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4228 5129 5391 820 Size: 197 926 2285 901 262 1553 Fragments arranged by size: 2285 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 2408 1517 Size: 891 5233 StyI C'CwwG_G Cuts at: 513 1065 3563 513 Size: 552 2498 3074 Fragments arranged by size: 3074 2498 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3359 4151 4168 4321 4506 4951 325 Size: 1255 1779 792 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 792 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2164 4370 6082 Size: 274 80 33 51 1443 187 2206 1712 Cuts at: 6082 96 Size: 138 Fragments arranged by size: 2206 1712 1443 274 187 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2494 2526 6084 6116 2494 Size: 32 3558 32 2502 Fragments arranged by size: 3558 2502 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI