Restriction map for
pFN21AB7667
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 514 AAATTC [GA]^AATT[CT]
AhdI 305 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 465 GGATC GGATC(4/5)
AocI 307 CCTGAGG CC^T[ACGT]AGG
Aor51HI 284 AGCGCT AGC^GCT
ApoI 514 AAATTC [GA]^AATT[CT]
Asp700I 831 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 305 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 338 TGGCCA TGG^CCA
BanI 66 GGTGCC G^G[CT][GA]CC
BanII 147 GAGCCC G[GA]GC[CT]^C
BclI 407 TGATCA T^GATCA
BcnI 51 CCGGG CC^[GC]GG
BfaI 922 CTAG C^TAG
BsgI 744 CTGCAC GTGCAG(16/14)
BsiYI 865 CCTGGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 865 CCTGGTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 557 TGTACA T^GTACA
BspMI 813 GCAGGT ACCTGC(4/8)
BsrDI 561 CATTGC GCAATG(2/0)
BsrGI 557 TGTACA T^GTACA
BstEII 694 GGTTACC G^GT[ACGT]ACC
BstF5I 954 GGATG GGATG(2/0)
BstPI 694 GGTTACC G^GT[ACGT]ACC
Bsu36I 307 CCTGAGG CC^T[ACGT]AGG
CfoI 285 GCGC GCG^C
EaeI 338 TGGCCA [CT]^GGCC[GA]
Eam1105I 305 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 284 AGCGCT AGC^GCT
Eco81I 307 CCTGAGG CC^T[ACGT]AGG
EcoO65I 694 GGTTACC G^GT[ACGT]ACC
FbaI 407 TGATCA T^GATCA
FokI 954 GGATG GGATG(9/13)
HaeII 284 AGCGCT [GA]GCGC^[CT]
HhaI 285 GCGC GCG^C
HinP1I 285 GCGC G^CGC
MaeI 922 CTAG C^TAG
MaeII 116 ACGT A^CGT
MluNI 338 TGGCCA TGG^CCA
MscI 338 TGGCCA TGG^CCA
MspA1I 420 CCGCTG C[AC]G^C[GT]G
MwoI 425 GCCACCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 51 CCGGG CC^[GC]GG
NheI 921 GCTAGC G^CTAGC
NspI 662 GCATGT [GA]CATG^[CT]
PleI 904 GAGTC GAGTC(4/5)
PshAI 394 GACCGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 324 GATGC GCATC(5/9)
SspBI 557 TGTACA T^GTACA
StuI 737 AGGCCT AGG^CCT
XcmI 810 CCAGCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 831 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AgeI 351 ACCGGT A^CCGGT
395 ACCGGT
AspHI 73 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
BbsI 943 GAAGAC GAAGAC(2/6)
574 GTCTTC
BpmI 749 CTCCAG CTGGAG(16/14)
597 CTGGAG
BpuAI 943 GAAGAC GAAGAC(2/6)
574 GTCTTC
BsaWI 351 ACCGGT [AT]^CCGG[AT]
395 ACCGGT
BsiHKAI 73 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
BsrFI 351 ACCGGT [GA]^CCGG[CT]
395 ACCGGT
Cac8I 658 GCCTGC GC[ACGT]^[ACGT]GC
921 GCTAGC
Cfr10I 351 ACCGGT [GA]^CCGG[CT]
395 ACCGGT
DpnII 408 GATC ^GATC
466 GATC
EcoT14I 478 CCTTGG C^C[AT][AT]GG
789 CCTTGG
HphI 135 GGTGA GGTGA(8/7)
868 GGTGA
MboI 408 GATC ^GATC
466 GATC
MslI 189 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
747 CACTCCAGTG
NdeII 408 GATC ^GATC
466 GATC
NlaIV 66 GGTGCC GG[ACGT]^[ACGT]CC
856 GGTTCC
PinAI 351 ACCGGT A^CCGGT
395 ACCGGT
Sau3AI 408 GATC ^GATC
466 GATC
StyI 478 CCTTGG C^C[AT][AT]GG
789 CCTTGG
TfiI 386 GATTC G^A[AT]TC
684 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboII MnlI MseI MspI
MvaI NlaIII RsaI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI