Restriction map for
pFN21AB7667
(Circular) (Six-base) MAPSORT of: pFN21AB7667.seq Check: 8073 from: 1 to: 5716
pFN21AB7667 5716 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2809 2809
Size: 5716
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3526 278
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3059 1937
Size: 1122 4594
Acc65I G'GTAC_C
Cuts at: 3037 3037
Size: 5716
AclI AA'CG_TT
Cuts at: 1563 3843 4216 1563
Size: 2280 373 3063
Fragments arranged by size:
3063 2280 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 3779 5163 5431 1632
Size: 934 1213 1384 268 1917
Fragments arranged by size:
1917 1384 1213 934 268
AfeI AGC'GCT
Cuts at: 2291 2291
Size: 5716
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5519
AflIII A'CryG_T
Cuts at: 1203 4615 5592 1203
Size: 3412 977 1327
Fragments arranged by size:
3412 1327 977
AgeI A'CCGG_T
Cuts at: 2356 2400 4547 2356
Size: 44 2147 3525
Fragments arranged by size:
3525 2147 44
AhdI GACnn_n'nnGTC
Cuts at: 2315 4445 2315
Size: 2130 3586
AleI CACnn'nnGTG
Cuts at: 1166 2756 1166
Size: 1590 4126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5716
AlwNI CAG_nnn'CTG
Cuts at: 3459 5031 3459
Size: 1572 4144
ApaLI G'TGCA_C
Cuts at: 3773 4929 3773
Size: 1156 4560
ApoI r'AATT_y
Cuts at: 1493 2519 3025 3251 3403 5512 1493
Size: 1026 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 506 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4270 160
Size: 872 3238 1606
Fragments arranged by size:
3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
AvaI C'yCGr_G
Cuts at: 1870 1943 3041 1870
Size: 73 1098 4545
Fragments arranged by size:
4545 1098 73
BamHI G'GATC_C
Cuts at: 3046 3413 3046
Size: 367 5349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 3037 4492 618
Size: 325 217 627 284 966 1455 1842
Fragments arranged by size:
1842 1455 966 627 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2156 3035 1391
Size: 765 879 4072
Fragments arranged by size:
4072 879 765
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2572 2955 928
Size: 971 673 383 3689
Fragments arranged by size:
3689 971 673 383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3907 3941 1903
Size: 34 50 34 1886 34 3678
Fragments arranged by size:
3678 1886 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4818 3608
Size: 1210 4506
BclI T'GATC_A
Cuts at: 1548 2412 1548
Size: 864 4852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4327 136
Size: 107 122 71 631 605 2655 1525
Fragments arranged by size:
2655 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5711 5711
Size: 5716
BlpI GC'TnA_GC
Cuts at: 3133 3133
Size: 5716
Bme1580I G_kGCm'C
Cuts at: 1790 3777 4933 1790
Size: 1987 1156 2573
Fragments arranged by size:
2573 1987 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5716
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2613 3447 4405
Size: 416 53 83 399 24 1196 834 958
Cuts at: 4405 442
Size: 1753
Fragments arranged by size:
1753 1196 958 834 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2930 1056
Size: 1874 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2623 2739 4376 1239
Size: 1384 116 1637 2579
Fragments arranged by size:
2579 1637 1384 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3841 4706 5004 3841
Size: 865 298 4553
Fragments arranged by size:
4553 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4379 882
Size: 617 2880 2219
Fragments arranged by size:
2880 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3507 493
Size: 711 2303 2702
Fragments arranged by size:
2702 2303 711
BsaBI GATnn'nnATC
Cuts at: 5710 5710
Size: 5716
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3523 3905 4540 275
Size: 53 83 186 936 1990 382 635 1451
Fragments arranged by size:
1990 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2356 2400 4149 4547 4821 4968 5379 2356
Size: 44 1749 398 274 147 411 2693
Fragments arranged by size:
2693 1749 411 398 274 147 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5491
Fragments arranged by size:
5491 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5716
BseYI C'CCAG_C
Cuts at: 2155 4919 5415 2155
Size: 2764 496 2456
Fragments arranged by size:
2764 2456 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2734 1163
Size: 573 998 4145
Fragments arranged by size:
4145 998 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3166 3927 4955 1540
Size: 275 186 1165 761 1028 2301
Fragments arranged by size:
2301 1165 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2103 3777 3862 4933 2082
Size: 21 1674 85 1071 2865
Fragments arranged by size:
2865 1674 1071 85 21
BsmI GAATG_Cn'
Cuts at: 3227 3320 3227
Size: 93 5623
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2103 2156 3035 3777 3862 4933
Size: 399 292 21 53 879 742 85 1071
Cuts at: 4933 1391
Size: 2174
Fragments arranged by size:
2174 1071 879 742 399 292 85 53
21
BspEI T'CCGG_A
Cuts at: 5379 5379
Size: 5716
BspHI T'CATG_A
Cuts at: 3605 5335 3605
Size: 1730 3986
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2809 3071 844
Size: 1965 262 3489
Fragments arranged by size:
3489 1965 262
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 1394
Size: 429 1780 3507
Fragments arranged by size:
3507 1780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2565 4211 4385 65
Size: 1183 1317 1646 174 1396
Fragments arranged by size:
1646 1396 1317 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2356 2400 4360 4547 1700
Size: 110 546 44 1960 187 2869
Fragments arranged by size:
2869 1960 546 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 2562 96
Size: 2466 3250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5716
BssSI C'ACGA_G
Cuts at: 3776 4788 3776
Size: 1012 4704
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5716
BstBI TT'CG_AA
Cuts at: 4520 4520
Size: 5716
BstEII G'GTnAC_C
Cuts at: 1184 2699 1184
Size: 1515 4201
BstYI r'GATC_y
Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711
Size: 1074 367 385 17 1452 86 12 346
Cuts at: 5711 1972
Size: 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2313 2313
Size: 5716
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4977
Fragments arranged by size:
4977 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2476 2921 3232 4026 4046 2434
Size: 42 445 311 794 20 4104
Fragments arranged by size:
4104 794 445 311 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5681
DraI TTT'AAA
Cuts at: 3020 3020
Size: 5716
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4723 809
Size: 507 3407 1802
Fragments arranged by size:
3407 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3163 4052
Size: 54 1425 50 275 18 513 820 889
Cuts at: 4052 5681 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 820 513 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3163 1537
Size: 275 1351 4090
Fragments arranged by size:
4090 1351 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3646 1353
Size: 2293 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4303 4677 4823 387
Size: 1064 138 2714 374 146 1280
Fragments arranged by size:
2714 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 2623 2739 3779 4376 5163 5431
Size: 393 934 57 116 1040 597 787 268
Cuts at: 5431 1239
Size: 1524
Fragments arranged by size:
1524 1040 934 787 597 393 268 116
57
EcoRI G'AATT_C
Cuts at: 3025 3403 3025
Size: 378 5338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2284 2248
Size: 36 5680
FspI TGC'GCA
Cuts at: 4222 4222
Size: 5716
HaeII r_GCGC'y
Cuts at: 1678 2293 4863 1678
Size: 615 2570 2531
Fragments arranged by size:
2570 2531 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2301 2333 2867 2899 4357
Size: 32 135 32 729 32 534 32 1458
Cuts at: 4357 4389 4431 4463 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1458 729 534 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3060 3306 669
Size: 988 281 1122 246 3079
Fragments arranged by size:
3079 1122 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5716
HpaI GTT'AAC
Cuts at: 3306 3306
Size: 5716
KpnI G_GTAC'C
Cuts at: 3041 3041
Size: 5716
MfeI C'AATT_G
Cuts at: 3315 3315
Size: 5716
MluI A'CGCG_T
Cuts at: 5592 5592
Size: 5716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 4830 5014 1305
Size: 1230 2295 184 2007
Fragments arranged by size:
2295 2007 1230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 10
Size: 54 1425 343 513 3381
Fragments arranged by size:
3381 1425 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2756 3674 4033 4192 518
Size: 648 1032 558 918 359 159 2042
Fragments arranged by size:
2042 1032 918 648 558 359 159
MspA1I CmG'CkG
Cuts at: 1606 2427 3132 3809 4957 5202 1606
Size: 821 705 677 1148 245 2120
Fragments arranged by size:
2120 1148 821 705 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5164
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5716
NheI G'CTAG_C
Cuts at: 1052 2926 1052
Size: 1874 3842
NotI GC'GGCC_GC
Cuts at: 3163 3163
Size: 5716
NspI r_CATG'y
Cuts at: 1132 1229 2671 3074 4619 1132
Size: 97 1442 403 1545 2229
Fragments arranged by size:
2229 1545 1442 403 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5716
PciI A'CATG_T
Cuts at: 4615 4615
Size: 5716
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5716
PmeI GTTT'AAAC
Cuts at: 3020 3020
Size: 5716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 5298 5330 3785
Size: 32 1481 32 4171
Fragments arranged by size:
4171 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2403 1613
Size: 790 4926
PsiI TTA'TAA
Cuts at: 3286 3286
Size: 5716
PstI C_TGCA'G
Cuts at: 830 3068 830
Size: 2238 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5716
SalI G'TCGA_C
Cuts at: 1936 3058 1936
Size: 1122 4594
SbfI CC_TGCA'GG
Cuts at: 3068 3068
Size: 5716
ScaI AGT'ACT
Cuts at: 3964 5676 3964
Size: 1712 4004
SfcI C'TryA_G
Cuts at: 826 1045 3064 4199 4880 5071 826
Size: 219 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5716
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5716
SmaI CCC'GGG
Cuts at: 1872 3043 1872
Size: 1171 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3820 4721 4983 820
Size: 197 926 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3507 493
Size: 3014 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5716
SphI G_CATG'C
Cuts at: 1132 3074 1132
Size: 1942 3774
SspI AAT'ATT
Cuts at: 5 52 3640 5
Size: 47 3588 2081
Fragments arranged by size:
3588 2081 47
StuI AGG'CCT
Cuts at: 1517 2744 1517
Size: 1227 4489
StyI C'CwwG_G
Cuts at: 513 1065 2483 2794 3155 513
Size: 552 1418 311 361 3074
Fragments arranged by size:
3074 1418 552 361 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2059 3743 3760 3913 4098 4543 325
Size: 1255 479 1684 17 153 185 445 1498
Fragments arranged by size:
1684 1498 1255 479 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2562 3962 5674
Size: 274 80 33 51 1443 585 1400 1712
Cuts at: 5674 96
Size: 138
Fragments arranged by size:
1712 1443 1400 585 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5676 5708 5676
Size: 32 5684
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5716
XbaI T'CTAG_A
Cuts at: 3052 3052
Size: 5716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2822 2822
Size: 5716
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5716
XmaI C'CCGG_G
Cuts at: 1870 3041 1870
Size: 1171 4545
XmnI GAAnn'nnTTC
Cuts at: 2840 3845 2840
Size: 1005 4711
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3524 276
Size: 53 83 186 936 1990 2468
Fragments arranged by size:
2468 1990 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI