(Circular) (Six-base) MAPSORT of: pFN21AB7627.seq Check: 7471 from: 1 to: 5596 pFN21AB7627 5596 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2063 2063 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3659 5043 5311 1632 Size: 2027 1384 268 1917 Fragments arranged by size: 2027 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 2048 4325 2048 Size: 2277 3319 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaLI G'TGCA_C Cuts at: 2323 3653 4809 2323 Size: 1330 1156 3110 Fragments arranged by size: 3110 1330 1156 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2657 2921 1870 Size: 73 714 264 4545 Fragments arranged by size: 4545 714 264 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4372 618 Size: 325 217 627 1130 1455 1842 Fragments arranged by size: 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 2475 2868 2915 1391 Size: 1084 393 47 4072 Fragments arranged by size: 4072 1084 393 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2327 2403 2877 3657 4813 1790 Size: 537 76 474 780 1156 2573 Fragments arranged by size: 2573 1156 780 537 474 76 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 2752 3327 Size: 416 53 83 399 24 621 714 575 Cuts at: 3327 4285 442 Size: 958 1753 Fragments arranged by size: 1753 958 714 621 575 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2832 2871 2882 4256 1239 Size: 1593 39 11 1374 2579 Fragments arranged by size: 2579 1593 1374 39 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2588 2813 4259 882 Size: 617 1089 225 1446 2219 Fragments arranged by size: 2219 1446 1089 617 225 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 1186 Size: 944 4652 BseYI C'CCAG_C Cuts at: 2160 2585 2637 2888 4799 5295 2160 Size: 425 52 251 1911 496 2461 Fragments arranged by size: 2461 1911 496 425 251 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2110 2212 2704 1163 Size: 573 374 102 492 4055 Fragments arranged by size: 4055 573 492 374 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2327 2868 3657 3742 4813 2327 Size: 541 789 85 1071 3110 Fragments arranged by size: 3110 1071 789 541 85 BsmI GAATG_Cn' Cuts at: 2768 3107 3200 2768 Size: 339 93 5164 Fragments arranged by size: 5164 339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2327 2403 2475 2868 2877 2915 3657 Size: 399 537 76 72 393 9 38 742 Cuts at: 3657 3742 4813 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 393 85 76 72 38 9 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2063 2136 2787 2951 844 Size: 1219 73 651 164 3489 Fragments arranged by size: 3489 1219 651 164 73 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2509 4091 4265 65 Size: 1183 1261 1582 174 1396 Fragments arranged by size: 1582 1396 1261 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2133 2322 794 Size: 1339 189 4068 Fragments arranged by size: 4068 1339 189 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2066 2066 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591 Size: 954 367 385 17 1452 86 12 346 Cuts at: 5591 1972 Size: 1977 Fragments arranged by size: 1977 1452 954 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2406 2406 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2004 2709 513 Size: 552 939 705 3400 Fragments arranged by size: 3400 939 705 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2077 2112 2404 2439 529 Size: 35 1513 35 292 35 3686 Fragments arranged by size: 3686 1513 292 35 35 35 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561 Size: 54 1425 50 275 18 1213 889 1629 Cuts at: 5561 8 Size: 43 Fragments arranged by size: 1629 1425 1213 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2480 2576 3526 1353 Size: 1127 96 950 3423 Fragments arranged by size: 3423 1127 950 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2866 2866 Size: 5596 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2832 2871 2882 3659 4256 5043 5311 Size: 393 1200 39 11 777 597 787 268 Cuts at: 5311 1239 Size: 1524 Fragments arranged by size: 1524 1200 787 777 597 393 268 39 11 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2657 2689 4237 Size: 32 135 32 462 32 591 32 1548 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1548 591 462 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2224 2940 3186 669 Size: 988 281 286 716 246 3079 Fragments arranged by size: 3079 988 716 286 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3554 3913 4072 518 Size: 648 2388 359 159 2042 Fragments arranged by size: 2388 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 3012 3689 4837 5082 1606 Size: 554 852 677 1148 245 2120 Fragments arranged by size: 2120 1148 852 677 554 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2709 513 Size: 552 939 705 3400 Fragments arranged by size: 3400 939 705 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PfoI T'CCnGG_A Cuts at: 2071 2071 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PspXI vC'TCGA_Gb Cuts at: 2657 2657 Size: 5596 PstI C_TGCA'G Cuts at: 830 2304 2535 2672 2948 830 Size: 1474 231 137 276 3478 Fragments arranged by size: 3478 1474 276 231 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2160 2160 Size: 5596 SacI G_AGCT'C Cuts at: 2868 2868 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2480 2480 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2304 2948 2304 Size: 644 4952 ScaI AGT'ACT Cuts at: 2027 3844 5556 2027 Size: 1817 1712 2067 Fragments arranged by size: 2067 1817 1712 SfcI C'TryA_G Cuts at: 826 1045 2300 2531 2668 2944 4079 4760 4951 Size: 219 1255 231 137 276 1135 681 191 Cuts at: 4951 826 Size: 1471 Fragments arranged by size: 1471 1255 1135 681 276 231 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3700 4601 4863 820 Size: 197 926 714 1043 901 262 1553 Fragments arranged by size: 1553 1043 926 901 714 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 2678 152 Size: 2526 3070 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 2298 1517 Size: 781 4815 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2709 2823 3035 513 Size: 552 939 84 621 114 212 3074 Fragments arranged by size: 3074 939 621 552 212 114 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2407 2599 3623 3640 3793 3978 4423 Size: 1255 827 192 1024 17 153 185 445 Cuts at: 4423 325 Size: 1498 Fragments arranged by size: 1498 1255 1024 827 445 192 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 3842 5554 Size: 274 80 33 51 1443 48 1817 1712 Cuts at: 5554 96 Size: 138 Fragments arranged by size: 1817 1712 1443 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 5556 5588 2297 Size: 32 3227 32 2305 Fragments arranged by size: 3227 2305 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 2657 1943 Size: 714 4882 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI