Restriction map for
pFN21AB7602
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraII DrdI Eam1105I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MaeIII MamI MfeI
MflI MroI MseI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 506 GTAC GT^AC
AflIII 136 ACGCGT A^C[GA][CT]GT
AocI 866 CCTCAGG CC^T[ACGT]AGG
Aor51HI 620 AGCGCT AGC^GCT
ApaI 55 GGGCCC GGGCC^C
AviII 843 TGCGCA TGC^GCA
BalI 372 TGGCCA TGG^CCA
BglI 677 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 73 CTGCAC GTGCAG(16/14)
BsiEI 488 CGGCCG CG[GA][CT]^CG
BsmFI 465 GGGAC GGGAC(10/14)
BspMI 227 ACCTGC ACCTGC(4/8)
BssSI 609 CTCGTG CACGAG(-5/-1)
BstAPI 454 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 80 CATCC GGATG(2/0)
Bsu36I 866 CCTCAGG CC^T[ACGT]AGG
DpnII 222 GATC ^GATC
DraIII 606 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 488 CGGCCG C^GGCCG
EclXI 488 CGGCCG C^GGCCG
Eco47III 620 AGCGCT AGC^GCT
Eco52I 488 CGGCCG C^GGCCG
Eco81I 866 CCTCAGG CC^T[ACGT]AGG
FokI 80 CATCC GGATG(9/13)
FspI 843 TGCGCA TGC^GCA
KspI 103 CCGCGG CCGC^GG
MaeII 152 ACGT A^CGT
MboI 222 GATC ^GATC
MluI 136 ACGCGT A^CGCGT
MluNI 372 TGGCCA TGG^CCA
MscI 372 TGGCCA TGG^CCA
NdeII 222 GATC ^GATC
NotI 487 GCGGCCGC GC^GGCCGC
NspI 840 ACATGC [GA]CATG^[CT]
PaeR7I 157 CTCGAG C^TCGAG
RsaI 506 GTAC GT^AC
SacI 351 GAGCTC GAGCT^C
SacII 103 CCGCGG CCGC^GG
Sau3AI 222 GATC ^GATC
TaqI 158 TCGA T^CGA
Tsp509I 377 AATT ^AATT
TthHB8I 158 TCGA T^CGA
XbaI 238 TCTAGA T^CTAGA
XcmI 903 CCAAGGCTACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 157 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 446 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
455 CAGCGGCTG
AspHI 154 GTGCTC G[AT]GC[AT]^C
351 GAGCTC
AvaI 157 CTCGAG C^[CT]CG[GA]G
354 CTCGGG
AvaII 66 GGACC G^G[AT]CC
1032 GGACC
BbeI 637 GGCGCC GGCGC^C
967 GGCGCC
BlpI 554 GCTCAGC GC^T[ACGT]AGC
789 GCTCAGC
BpmI 338 CTCCAG CTGGAG(16/14)
528 CTGGAG
Bpu1102I 554 GCTCAGC GC^T[ACGT]AGC
789 GCTCAGC
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
351 GAGCTC
BsoBI 157 CTCGAG C^[CT]CG[GA]G
354 CTCGGG
BsrBI 294 GAGCGG CCGCTC(-3/-3)
300 GAGCGG
BsrI 340 CCAGT ACTGG(1/-1)
861 CCAGT
CelII 554 GCTCAGC GC^T[ACGT]AGC
789 GCTCAGC
KasI 637 GGCGCC G^GCGCC
967 GGCGCC
MslI 192 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
835 CACCCACATG
NarI 637 GGCGCC GG^CGCC
967 GGCGCC
SapI 165 GCTCTTC GCTCTTC(1/4)
431 GCTCTTC
SfoI 637 GGCGCC GGC^GCC
967 GGCGCC
SmlI 157 CTCGAG C^T[CT][GA]AG
217 CTCAAG
TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^
369 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NlaIII NlaIV PleI
PstI PvuII Sau96I ScrFI SfcI
StyI TseI