(Circular) (Six-base) MAPSORT of: pFN21AB7602.seq Check: 8494 from: 1 to: 5767 pFN21AB7602 5767 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 1937 Size: 1173 4594 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3830 5214 5482 1632 Size: 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 268 AfeI AGC'GCT Cuts at: 2627 2627 Size: 5767 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 2141 4666 5643 1203 Size: 938 2525 977 1327 Fragments arranged by size: 2525 1327 977 938 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2456 2465 3510 5082 2456 Size: 9 1045 1572 3141 Fragments arranged by size: 3141 1572 1045 9 ApaI G_GGCC'C Cuts at: 2064 2064 Size: 5767 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2359 3092 1870 Size: 73 219 197 733 4545 Fragments arranged by size: 4545 733 219 197 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 2642 2806 2972 3088 Size: 325 217 627 794 61 164 166 116 Cuts at: 3088 4543 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 794 627 325 217 166 164 116 61 BanII G_rGCy'C Cuts at: 1391 2064 2360 2366 2540 3086 1391 Size: 673 296 6 174 546 4072 Fragments arranged by size: 4072 673 546 296 174 6 BbeI G_GCGC'C Cuts at: 2646 2976 2646 Size: 330 5437 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2688 4378 136 Size: 107 122 71 631 605 1016 1690 1525 Fragments arranged by size: 1690 1525 1016 631 605 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 2560 2795 3184 2560 Size: 235 389 5143 Fragments arranged by size: 5143 389 235 Bme1580I G_kGCm'C Cuts at: 1790 2064 3828 4984 1790 Size: 274 1764 1156 2573 Fragments arranged by size: 2573 1764 1156 274 BmgBI CAC'GTC Cuts at: 1318 2158 1318 Size: 840 4927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2328 2554 4427 1239 Size: 1089 226 1873 2579 Fragments arranged by size: 2579 1873 1089 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 3892 4757 5055 2207 Size: 1685 865 298 2919 Fragments arranged by size: 2919 1685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2429 2643 2973 3574 Size: 53 83 186 936 896 214 330 601 Cuts at: 3574 3956 4591 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 896 635 601 382 330 214 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2996 3026 1178 Size: 30 165 30 1593 30 3919 Fragments arranged by size: 3919 1593 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2225 2710 2803 1186 Size: 1039 485 93 4150 Fragments arranged by size: 4150 1039 485 93 BseYI C'CCAG_C Cuts at: 2047 2323 2449 4970 5466 2047 Size: 276 126 2521 496 2348 Fragments arranged by size: 2521 2348 496 276 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 1163 Size: 573 327 4867 Fragments arranged by size: 4867 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2496 3217 3978 5006 1540 Size: 275 186 495 721 761 1028 2301 Fragments arranged by size: 2301 1028 761 721 495 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 2360 3828 3913 4984 2163 Size: 197 1468 85 1071 2946 Fragments arranged by size: 2946 1468 1071 197 85 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2163 2360 2366 2540 3086 3828 Size: 399 274 99 197 6 174 546 742 Cuts at: 3828 3913 4984 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 274 197 174 99 85 6 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 3122 844 Size: 1397 881 3489 Fragments arranged by size: 3489 1397 881 BsrBI CCG'CTC Cuts at: 1394 1823 2301 2307 3654 1394 Size: 429 478 6 1347 3507 Fragments arranged by size: 3507 1347 478 429 6 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2614 3827 4839 2614 Size: 1213 1012 3542 Fragments arranged by size: 3542 1213 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2465 794 Size: 1671 4096 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstYI r'GATC_y Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762 Size: 1125 367 385 17 1452 86 12 346 Cuts at: 5762 1972 Size: 1977 Fragments arranged by size: 1977 1452 1125 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2872 2872 Size: 5767 BtgI C'CryG_G Cuts at: 513 1065 2067 2088 2108 2802 2817 513 Size: 552 1002 21 20 694 15 3463 Fragments arranged by size: 3463 1002 694 552 21 20 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2380 3283 4077 4097 2380 Size: 903 794 20 4050 Fragments arranged by size: 4050 903 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DraIII CAC_nnn'GTG Cuts at: 2616 2616 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2100 2377 Size: 54 1425 50 275 18 225 45 277 Cuts at: 2377 2493 2595 2680 2698 3214 4103 5732 8 Size: 116 102 85 18 516 889 1629 43 Fragments arranged by size: 1629 1425 889 516 277 275 225 116 102 85 54 50 45 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2493 3214 1537 Size: 275 681 721 4090 Fragments arranged by size: 4090 721 681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 2443 2728 3697 1353 Size: 824 266 285 969 3423 Fragments arranged by size: 3423 969 824 285 266 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2431 2980 4354 4728 4874 387 Size: 1064 138 842 549 1374 374 146 1280 Fragments arranged by size: 1374 1280 1064 842 549 374 146 138 EcoICRI GAG'CTC Cuts at: 2358 2358 Size: 5767 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2554 3830 4427 5214 5482 1239 Size: 393 696 226 1276 597 787 268 1524 Fragments arranged by size: 1524 1276 787 696 597 393 268 226 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FspI TGC'GCA Cuts at: 2850 4273 2850 Size: 1423 4344 FspAI rTGC'GCAy Cuts at: 2850 2850 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2171 2222 2567 2629 2646 2765 2976 4914 Size: 493 51 345 62 17 119 211 1938 Cuts at: 4914 1678 Size: 2531 Fragments arranged by size: 2531 1938 493 345 211 119 62 51 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2238 2270 2735 2767 3029 Size: 32 135 32 666 32 465 32 262 Cuts at: 3029 3061 4408 4440 4482 4514 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 666 465 262 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KasI G'GCGC_C Cuts at: 2642 2972 2642 Size: 330 5437 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 2141 5643 2141 Size: 3502 2265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2379 10 Size: 54 1425 343 547 3398 Fragments arranged by size: 3398 1425 547 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2844 3725 4084 4243 518 Size: 648 1035 643 881 359 159 2042 Fragments arranged by size: 2042 1035 881 648 643 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2119 2152 2396 2453 2462 2519 2978 Size: 504 9 33 244 57 9 57 459 Cuts at: 2978 3183 3860 5008 5253 1606 Size: 205 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 504 459 245 244 205 57 57 33 9 9 NarI GG'CG_CC Cuts at: 2643 2973 2643 Size: 330 5437 NcoI C'CATG_G Cuts at: 513 1065 2067 2802 2817 513 Size: 552 1002 735 15 3463 Fragments arranged by size: 3463 1002 735 552 15 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 2493 3214 2493 Size: 721 5046 NspI r_CATG'y Cuts at: 1132 1229 2849 3125 4670 1132 Size: 97 1620 276 1545 2229 Fragments arranged by size: 2229 1620 1545 276 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 3064 1108 Size: 1956 3811 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspOMI G'GGCC_C Cuts at: 2060 2060 Size: 5767 PspXI vC'TCGA_Gb Cuts at: 2162 2162 Size: 5767 PstI C_TGCA'G Cuts at: 830 2461 2641 2750 3119 830 Size: 1631 180 109 369 3478 Fragments arranged by size: 3478 1631 369 180 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2152 2396 2519 2152 Size: 244 123 5400 Fragments arranged by size: 5400 244 123 SacI G_AGCT'C Cuts at: 2360 2360 Size: 5767 SacII CC_GC'GG Cuts at: 2111 2111 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SapI GCTCTTCn'nnn_ Cuts at: 2177 2443 2177 Size: 266 5501 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2457 2637 2746 3115 4250 4931 5122 Size: 219 1412 180 109 369 1135 681 191 Cuts at: 5122 826 Size: 1471 Fragments arranged by size: 1471 1412 1135 681 369 219 191 180 109 SfoI GGC'GCC Cuts at: 2644 2974 2644 Size: 330 5437 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2222 3871 4772 5034 820 Size: 197 926 219 60 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 219 197 60 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2067 2701 2802 2817 2908 3206 513 Size: 552 1002 634 101 15 91 298 3074 Fragments arranged by size: 3074 1002 634 552 298 101 91 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2097 2856 3794 3811 3964 4149 4594 Size: 1255 517 759 938 17 153 185 445 Cuts at: 4594 325 Size: 1498 Fragments arranged by size: 1498 1255 938 759 517 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2510 4013 5725 Size: 274 80 33 51 1443 533 1503 1712 Cuts at: 5725 96 Size: 138 Fragments arranged by size: 1712 1503 1443 533 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5727 5759 5727 Size: 32 5735 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 2243 3103 2243 Size: 860 4907 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2915 2915 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 2162 1943 Size: 219 5548 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SanDI SexAI SfiI SrfI SwaI