Restriction map for
pFN21AB7602
(Linear) (Six-base) MAPSORT of: KIBB7602.seq Check: 671 from: 1 to: 1062
KIBB7602.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AfeI AGC'GCT
Cuts at: 0 622 1062
Size: 622 440
AflIII A'CryG_T
Cuts at: 0 136 1062
Size: 136 926
AlwNI CAG_nnn'CTG
Cuts at: 0 451 460 1062
Size: 451 9 602
Fragments arranged by size:
602 451 9
ApaI G_GGCC'C
Cuts at: 0 59 1062
Size: 59 1003
AvaI C'yCGr_G
Cuts at: 0 157 354 1062
Size: 157 197 708
Fragments arranged by size:
708 197 157
BanI G'GyrC_C
Cuts at: 0 576 637 801 967 1062
Size: 576 61 164 166 95
Fragments arranged by size:
576 166 164 95 61
BanII G_rGCy'C
Cuts at: 0 59 355 361 535 1062
Size: 59 296 6 174 527
Fragments arranged by size:
527 296 174 59 6
BbeI G_GCGC'C
Cuts at: 0 641 971 1062
Size: 641 330 91
Fragments arranged by size:
641 330 91
BglI GCCn_nnn'nGGC
Cuts at: 0 683 1062
Size: 683 379
BlpI GC'TnA_GC
Cuts at: 0 555 790 1062
Size: 555 235 272
Fragments arranged by size:
555 272 235
Bme1580I G_kGCm'C
Cuts at: 0 59 1062
Size: 59 1003
BmgBI CAC'GTC
Cuts at: 0 153 1062
Size: 153 909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 549 1062
Size: 323 226 513
Fragments arranged by size:
513 323 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1062
Size: 202 860
BsaHI Gr'CG_yC
Cuts at: 0 424 638 968 1062
Size: 424 214 330 94
Fragments arranged by size:
424 330 214 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1062
Size: 991 30 41
Fragments arranged by size:
991 41 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 705 798 1062
Size: 220 485 93 264
Fragments arranged by size:
485 264 220 93
BseYI C'CCAG_C
Cuts at: 0 42 318 444 1062
Size: 42 276 126 618
Fragments arranged by size:
618 276 126 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1062
Size: 58 1004
BsiEI CG_ry'CG
Cuts at: 0 491 1062
Size: 491 571
BsiHKAI G_wGCw'C
Cuts at: 0 158 355 1062
Size: 158 197 707
Fragments arranged by size:
707 197 158
Bsp1286I G_dGCh'C
Cuts at: 0 59 158 355 361 535 1062
Size: 59 99 197 6 174 527
Fragments arranged by size:
527 197 174 99 59 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 1062
Size: 236 826
BsrBI CCG'CTC
Cuts at: 0 296 302 1062
Size: 296 6 760
Fragments arranged by size:
760 296 6
BssSI C'ACGA_G
Cuts at: 0 609 1062
Size: 609 453
BstAPI GCAn_nnn'nTGC
Cuts at: 0 460 1062
Size: 460 602
Bsu36I CC'TnA_GG
Cuts at: 0 867 1062
Size: 867 195
BtgI C'CryG_G
Cuts at: 0 62 83 103 797 812 1062
Size: 62 21 20 694 15 250
Fragments arranged by size:
694 250 62 21 20 15
BtsI GCAGTG_nn'
Cuts at: 0 375 1062
Size: 375 687
DraIII CAC_nnn'GTG
Cuts at: 0 611 1062
Size: 611 451
EaeI y'GGCC_r
Cuts at: 0 50 95 372 488 590 675 693 1062
Size: 50 45 277 116 102 85 18 369
Fragments arranged by size:
369 277 116 102 85 50 45 18
EagI C'GGCC_G
Cuts at: 0 488 1062
Size: 488 574
EarI CTCTTCn'nnn_
Cuts at: 0 172 438 723 1062
Size: 172 266 285 339
Fragments arranged by size:
339 285 266 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 426 975 1062
Size: 426 549 87
Fragments arranged by size:
549 426 87
EcoICRI GAG'CTC
Cuts at: 0 353 1062
Size: 353 709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 549 1062
Size: 323 226 513
Fragments arranged by size:
513 323 226
FspI TGC'GCA
Cuts at: 0 845 1062
Size: 845 217
FspAI rTGC'GCAy
Cuts at: 0 845 1062
Size: 845 217
HaeII r_GCGC'y
Cuts at: 0 166 217 562 624 641 760 971 1062
Size: 166 51 345 62 17 119 211 91
Fragments arranged by size:
345 211 166 119 91 62 51 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 233 265 730 762 1024 1056 1062
Size: 233 32 465 32 262 32 6
Fragments arranged by size:
465 262 233 32 32 32 6
KasI G'GCGC_C
Cuts at: 0 637 967 1062
Size: 637 330 95
Fragments arranged by size:
637 330 95
MluI A'CGCG_T
Cuts at: 0 136 1062
Size: 136 926
MscI TGG'CCA
Cuts at: 0 374 1062
Size: 374 688
MslI CAynn'nnrTG
Cuts at: 0 196 839 1062
Size: 196 643 223
Fragments arranged by size:
643 223 196
MspA1I CmG'CkG
Cuts at: 0 105 114 147 391 448 457 514 973
Size: 105 9 33 244 57 9 57 459
Cuts at: 973 1062
Size: 89
Fragments arranged by size:
459 244 105 89 57 57 33 9
9
NarI GG'CG_CC
Cuts at: 0 638 968 1062
Size: 638 330 94
Fragments arranged by size:
638 330 94
NcoI C'CATG_G
Cuts at: 0 62 797 812 1062
Size: 62 735 15 250
Fragments arranged by size:
735 250 62 15
NotI GC'GGCC_GC
Cuts at: 0 488 1062
Size: 488 574
NspI r_CATG'y
Cuts at: 0 844 1062
Size: 844 218
PspOMI G'GGCC_C
Cuts at: 0 55 1062
Size: 55 1007
PspXI vC'TCGA_Gb
Cuts at: 0 157 1062
Size: 157 905
PstI C_TGCA'G
Cuts at: 0 456 636 745 1062
Size: 456 180 109 317
Fragments arranged by size:
456 317 180 109
PvuII CAG'CTG
Cuts at: 0 147 391 514 1062
Size: 147 244 123 548
Fragments arranged by size:
548 244 147 123
SacI G_AGCT'C
Cuts at: 0 355 1062
Size: 355 707
SacII CC_GC'GG
Cuts at: 0 106 1062
Size: 106 956
SapI GCTCTTCn'nnn_
Cuts at: 0 172 438 1062
Size: 172 266 624
Fragments arranged by size:
624 266 172
SfcI C'TryA_G
Cuts at: 0 452 632 741 1062
Size: 452 180 109 321
Fragments arranged by size:
452 321 180 109
SfoI GGC'GCC
Cuts at: 0 639 969 1062
Size: 639 330 93
Fragments arranged by size:
639 330 93
SmlI C'TyrA_G
Cuts at: 0 157 217 1062
Size: 157 60 845
Fragments arranged by size:
845 157 60
StyI C'CwwG_G
Cuts at: 0 62 696 797 812 903 1062
Size: 62 634 101 15 91 159
Fragments arranged by size:
634 159 101 91 62 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 851 1062
Size: 92 759 211
Fragments arranged by size:
759 211 92
TatI w'GTAC_w
Cuts at: 0 505 1062
Size: 505 557
XbaI T'CTAG_A
Cuts at: 0 238 1062
Size: 238 824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 1062
Size: 910 152
XhoI C'TCGA_G
Cuts at: 0 157 1062
Size: 157 905
Enzymes that do cut:
AfeI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BssSI BstAPI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI FspI FspAI HaeII Hin4I
KasI MluI MscI MslI MspA1I NarI NcoI NotI
NspI PspOMI PspXI PstI PvuII SacI SacII SapI
SfcI SfoI SmlI StyI TaqII TatI XbaI XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoNI EcoO109I
EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI MmeI NaeI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XmaI XmnI ZraI