(Circular) (Six-base) MAPSORT of: pFN21AE0706.seq Check: 5480 from: 1 to: 5926 pFN21AE0706 5926 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3989 5373 5641 1632 Size: 2357 1384 268 1917 Fragments arranged by size: 2357 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 3223 4825 5802 1203 Size: 2020 1602 977 1327 Fragments arranged by size: 2020 1602 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 2373 1166 Size: 1207 4719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaI G_GGCC'C Cuts at: 3155 3155 Size: 5926 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2174 2361 3235 3461 3613 5722 1493 Size: 681 187 874 226 152 2109 1697 Fragments arranged by size: 2109 1697 874 681 226 187 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2154 2265 2463 2574 2951 3251 Size: 73 71 140 111 198 111 377 300 Cuts at: 3251 1870 Size: 4545 Fragments arranged by size: 4545 377 300 198 140 111 111 73 71 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2609 2713 2858 3247 Size: 325 217 627 231 591 104 145 389 Cuts at: 3247 4702 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 591 389 325 231 217 145 104 BanII G_rGCy'C Cuts at: 1391 2391 2770 3155 3245 1391 Size: 1000 379 385 90 4072 Fragments arranged by size: 4072 1000 385 379 90 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BfrBI ATG'CAT Cuts at: 2524 2524 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2680 4537 136 Size: 107 122 71 631 605 1008 1857 1525 Fragments arranged by size: 1857 1525 1008 631 605 122 107 71 BglII A'GATC_T Cuts at: 5921 5921 Size: 5926 BlpI GC'TnA_GC Cuts at: 2568 3024 3343 2568 Size: 456 319 5151 Fragments arranged by size: 5151 456 319 Bme1580I G_kGCm'C Cuts at: 1790 2640 2863 3155 3987 5143 1790 Size: 850 223 292 832 1156 2573 Fragments arranged by size: 2573 1156 850 832 292 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2838 4586 1239 Size: 841 758 1748 2579 Fragments arranged by size: 2579 1748 841 758 Bpu10I CC'TnA_GC Cuts at: 2390 2390 Size: 5926 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3091 4051 4916 5214 3091 Size: 960 865 298 3803 Fragments arranged by size: 3803 960 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2679 3717 493 Size: 711 1475 1038 2702 Fragments arranged by size: 2702 1475 1038 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 2445 3733 4115 Size: 53 83 186 936 486 426 1288 382 Cuts at: 4115 4750 275 Size: 635 1451 Fragments arranged by size: 1451 1288 936 635 486 426 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 1178 Size: 30 165 30 694 30 4977 Fragments arranged by size: 4977 694 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2778 2925 1186 Size: 1592 147 4187 Fragments arranged by size: 4187 1592 147 BseYI C'CCAG_C Cuts at: 2655 3101 5129 5625 2655 Size: 446 2028 496 2956 Fragments arranged by size: 2956 2028 496 446 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2294 3376 4137 5165 1540 Size: 275 186 293 1082 761 1028 2301 Fragments arranged by size: 2301 1082 1028 761 293 275 186 BsiHKAI G_wGCw'C Cuts at: 2066 2391 3987 4072 5143 2066 Size: 325 1596 85 1071 2849 Fragments arranged by size: 2849 1596 1071 325 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2991 2991 Size: 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2391 2640 2770 2863 3155 3245 Size: 399 276 325 249 130 93 292 90 Cuts at: 3245 3987 4072 5143 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 292 276 249 130 93 90 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 2127 2433 3815 5545 2127 Size: 306 1382 1730 2508 Fragments arranged by size: 2508 1730 1382 306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 3281 844 Size: 2200 237 3489 Fragments arranged by size: 3489 2200 237 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2716 3161 4570 4757 1700 Size: 110 906 445 1409 187 2869 Fragments arranged by size: 2869 1409 906 445 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5926 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 3256 3623 4008 4025 5477 5563 5575 5921 Size: 1284 367 385 17 1452 86 12 346 Cuts at: 5921 1972 Size: 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2847 2847 Size: 5926 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5374 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3442 4236 4256 3442 Size: 794 20 5112 Fragments arranged by size: 5112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2175 2210 2690 2725 529 Size: 35 1611 35 480 35 3730 Fragments arranged by size: 3730 1611 480 35 35 35 DraI TTT'AAA Cuts at: 2179 3230 2179 Size: 1051 4875 DraIII CAC_nnn'GTG Cuts at: 3199 3199 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2513 4933 809 Size: 507 1197 2420 1802 Fragments arranged by size: 2420 1802 1197 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 2233 2291 Size: 54 1425 50 275 18 378 25 58 Cuts at: 2291 3373 4262 5891 8 Size: 1082 889 1629 43 Fragments arranged by size: 1629 1425 1082 889 378 275 58 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2291 3373 1537 Size: 275 479 1082 4090 Fragments arranged by size: 4090 1082 479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2755 3856 1353 Size: 692 710 1101 3423 Fragments arranged by size: 3423 1101 710 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2389 2389 Size: 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2838 3989 4586 5373 5641 1239 Size: 393 448 758 1151 597 787 268 1524 Fragments arranged by size: 1524 1151 787 758 597 448 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2817 2817 Size: 5926 EcoO109I rG'GnC_Cy Cuts at: 2315 2460 3128 3151 3152 2315 Size: 145 668 23 1 5089 Fragments arranged by size: 5089 668 145 23 1 EcoRI G'AATT_C Cuts at: 2361 3235 3613 2361 Size: 874 378 4674 Fragments arranged by size: 4674 874 378 FspI TGC'GCA Cuts at: 2084 4432 2084 Size: 2348 3578 HaeII r_GCGC'y Cuts at: 1678 2022 2597 5073 1678 Size: 344 575 2476 2531 Fragments arranged by size: 2531 2476 575 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2483 2515 4567 4599 4641 Size: 32 135 32 911 32 2052 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 2052 911 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2518 3270 3516 669 Size: 988 281 580 752 246 3079 Fragments arranged by size: 3079 988 752 580 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 2371 3525 2371 Size: 1154 4772 MluI A'CGCG_T Cuts at: 3223 5802 3223 Size: 2579 3347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 3726 Fragments arranged by size: 3726 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2373 3884 4243 4402 518 Size: 648 1207 1511 359 159 2042 Fragments arranged by size: 2042 1511 1207 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3171 3342 4019 5167 5412 1606 Size: 1565 171 677 1148 245 2120 Fragments arranged by size: 2120 1565 1148 677 245 171 NaeI GCC'GGC Cuts at: 2718 3163 2718 Size: 445 5481 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5926 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 2716 3161 2716 Size: 445 5481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NsiI A_TGCA'T Cuts at: 2526 2526 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2524 3284 4829 1132 Size: 97 1295 760 1545 2229 Fragments arranged by size: 2229 1545 1295 760 97 PasI CC'CwG_GG Cuts at: 1348 2836 1348 Size: 1488 4438 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 2619 2655 1108 Size: 1511 36 4379 Fragments arranged by size: 4379 1511 36 PfoI T'CCnGG_A Cuts at: 2815 2815 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PmlI CAC'GTG Cuts at: 2679 2679 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 3128 2315 Size: 813 5113 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 3151 3151 Size: 5926 PstI C_TGCA'G Cuts at: 830 2758 3278 830 Size: 1928 520 3478 Fragments arranged by size: 3478 1928 520 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SacI G_AGCT'C Cuts at: 2391 2391 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SanDI GG'GwC_CC Cuts at: 3128 3128 Size: 5926 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 5926 SbfI CC_TGCA'GG Cuts at: 2758 3278 2758 Size: 520 5406 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2754 3274 4409 5090 5281 826 Size: 219 1709 520 1135 681 191 1471 Fragments arranged by size: 1709 1471 1135 681 520 219 191 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5926 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 3161 1700 Size: 1461 4465 SmaI CCC'GGG Cuts at: 1872 2267 2953 3253 1872 Size: 395 686 300 4545 Fragments arranged by size: 4545 686 395 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 2154 3070 4030 4931 5193 820 Size: 197 926 211 916 960 901 262 1553 Fragments arranged by size: 1553 960 926 916 901 262 211 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 3365 513 Size: 552 2300 3074 Fragments arranged by size: 3074 2300 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 3953 3970 4123 4308 4753 325 Size: 1255 1121 1252 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1252 1121 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4172 5884 96 Size: 274 80 33 51 1443 2195 1712 138 Fragments arranged by size: 2195 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2697 2729 5886 5918 2697 Size: 32 3157 32 2705 Fragments arranged by size: 3157 2705 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 2154 1943 Size: 211 5715 XmaI C'CCGG_G Cuts at: 1870 2265 2951 3251 1870 Size: 395 686 300 4545 Fragments arranged by size: 4545 686 395 300 XmnI GAAnn'nnTTC Cuts at: 2039 4055 2039 Size: 2016 3910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI PvuII RsrII SacII SexAI SfiI SrfI SwaI XcmI